FastQCFastQC Report
Mon 26 Jun 2023
SRR074370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9191720
Total Bases376.8 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA5544386.031928735862277No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAA3700434.025829768530808No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA3108323.381652182616529No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA2760273.0029961748182057No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA1993962.1693001962635936No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1661971.8081164352264862No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA1380201.5015688032272525No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAA1341431.459389537540308No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA1230061.3382261426588278No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA811180.8825116517909596No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAA781250.8499497373723307No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA743030.8083688363004965No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA685470.7457472594900627No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA539140.5865496338008556No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA536550.5837318804315188No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAA487170.5300096173512683No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA475660.5174874778605092No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA435270.4735457563981496No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAA415990.4525703567993803No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA408340.4442476489710304No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA394620.42932117166319256No Hit
TGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAA392230.42672100542662306No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA372520.40527779349240406No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA332890.36216290313456023No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA327840.35666882803218547No Hit
AAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAA304460.33123289221168617No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA288510.3138803183734927No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA285510.31061651138198293No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA273580.29763743891241246No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAA273080.2970934710804942No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAA270220.2939819750819216No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA265450.28879252196542105No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA257870.2805459696335397No Hit
TCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA235950.25669841988224185No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA221750.24124973345576237No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA221630.24111918117610195No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA220910.24033586749813962No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAA220470.23985717580605154No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAA217080.2361690739056455No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAA207040.22524619984072622No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA204800.2228092239537323No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA199360.21689085394246124No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA180610.1964920602455253No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA172100.187233727746276No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA167870.18263175988824726No Hit
TTAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAA165390.1799336794419325No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAA163100.17744230677174674No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAAA157350.1711866767046864No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAA156920.17071886436923667No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA154280.16784671421670808No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA153390.1668784514758935No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAA151760.1651051163438399No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA150380.1636037651277454No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA149850.16302715922591202No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAA149830.1630054005126353No Hit
TAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAAA147370.1603290787795973No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA145260.1580335345289021No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA141730.15419312163555896No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA141700.15416048356564385No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAA140230.15256121813980408No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAA138630.15082052107766555No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA137350.14942796342795472No Hit
AAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAAAAA135990.14794837092513696No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA132690.14435818323447625No Hit
TGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAA131380.1429329875148503No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA130930.14244341646612388No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA130600.14208439769705777Illumina PCR Primer Index 1 (95% over 22bp)
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA127200.1383854164400134No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA122400.1331633252535978No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA121520.1322059418694216No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAAAAAA114890.12499292841818507No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT111040.12080437611241422No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAA110890.12064118576283872No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAA110130.11981435465832292No Hit
GCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAA105380.11464666025509916No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA104270.11343905166824055No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA100300.10911994708280931No Hit
AGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAAAA99750.10852158246769919No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAA99640.10840190954467717No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAAA97900.10650890148960152No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA95640.10405016688933083No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA95290.10366938940698801No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAA92610.100753721827906No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA92200.10030766820573297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)