FastQCFastQC Report
Mon 26 Jun 2023
SRR074371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7369991
Total Bases302.1 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA1604832.1775196197661573No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA1542072.0923634777844367No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA1470761.9956062361541553No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA1401011.9009656863895763No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA1018271.3816434782620495No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA787451.0684544933636961No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA581510.7890240300157761No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAAAAAAAAAAAAA459310.6232165005357537No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAAAAAAAAAA456990.6200685998124014No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA417790.566879932417828No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAAAAAAAAAAA385020.5224158347004766No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA322550.4376531803091754No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAA320240.43451884812342373No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAA310900.4218458340044106No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA298130.4045188114883722No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAA270370.3668525511089498No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAA256250.34769377601682283No Hit
ACGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA254580.34542783023751317No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA236610.3210451681691334No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT235650.3197425885594704No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA234860.31867067408901856No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA228000.3093626572949682No Hit
AAAAAGCTCGTAGTTGGATCTTGGGAAAAAAAAAAAAAAAA227800.3090912865429551No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA225480.30594338581960273No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAA221320.30029887417772966No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACAAAAAAAAAAAAAA220520.29921339116967716No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA217400.29498000743827235No Hit
ACGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA211320.2867303365770732No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA204270.2771645175686103No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT196440.2665403526272963No Hit
ACGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA195060.2646678944384057No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT193590.26267331941110916No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA189550.25719163022044395No Hit
TCGACTGCATAATTTGTGGTAGTGGGCAAAAAAAAAAAAAA187500.25441008001230936No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA186310.25279542403783123No Hit
AGAATTCACCAAGCGTTGGATTGTTCAAAAAAAAAAAAAAA183930.24956611208887503No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA179610.2437045038453914No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA176590.23960680548999314No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAA176030.23884696738435637No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT171570.23279539961446358Illumina PCR Primer Index 1 (95% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA162880.22100434043949307No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA161290.2188469429609887No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA161140.21864341489697883No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT158280.2147628131431911Illumina Paired End PCR Primer 2 (95% over 21bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA157510.21371803574794052No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA157190.21328384254471955No Hit
TAGAATTCACCAAGCGTTGGATTGTTCAAAAAAAAAAAAAA154540.20968818008054554No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT153410.20815493533167137No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA153350.20807352410606741No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA152520.20694733548521296No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAA152300.2066488276579985No Hit
TCGACTGCATAATTTGTGGTAGTGGAGAAAAAAAAAAAAAA149680.2030938708066265No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA149170.20240187538899299No Hit
TCGACTGCATAATTTGTGGTAGTGGGAGAAAAAAAAAAAAA145270.19711014572473695No Hit
AGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA145260.1970965771871363No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA144710.1963503076191002No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA142160.1928903305309328No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT141980.19264609685412099No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT137820.18700158521224788Illumina PCR Primer Index 2 (95% over 21bp)
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT137760.18692017398664393Illumina Paired End PCR Primer 2 (95% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA137380.186404569557819No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACAAAAAAAAAAA136930.18579398536578948No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT136420.18510198994815596No Hit
ACGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA135580.18396223278970084No Hit
TATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAA135540.1839079586392982No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACCAAAAAAAAAA135460.18379941033849295No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA135330.18362301934968442No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA134430.18240185096562533No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA132080.17921324462947105No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA128010.17369084982600386No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAA126380.17147917819709685No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAAA126220.17126208159548634No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA125660.17050224348984958No Hit
GCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA125300.17001377613622595No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA124510.16894186166577407No Hit
AAGTGCGGGTCATAAGCTTGCGTTGATTAAAAAAAAAAAAA122670.16644525074725328No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT120760.1638536600655279Illumina Paired End PCR Primer 2 (95% over 21bp)
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT119310.1618862221134327Illumina PCR Primer Index 2 (95% over 21bp)
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAA115950.1573271934796121No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAA114890.15588892849394254No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA112660.15286314460899614No Hit
TGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAA109320.14833125305037687No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAAAAAAAAAAAA108740.14754427786953878No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA107620.14602460165826525No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAA106860.14499339280061535No Hit
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG105240.142795289709309Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGAAGTACGCACACACCGCCCGTCAAAAAAAAAAAAAAA105010.1424832133444939No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAA103710.14071930345640857No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAAA103220.14005444511397638No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCAAAAAAAAAAAAA100000.135685376006565No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA99340.13478985252492168No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA99250.13466773568651574No Hit
AGGGGAATCCGACTGTTTAATTAAAACAAAAAAAAAAAAAA98720.13394860319368096No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAAA98660.13386719196807703No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG96930.13151983496316344Illumina Paired End PCR Primer 2 (95% over 22bp)
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA96300.1306650170943221No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAA92070.12492552568924441No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAA90500.12279526528594133No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG90440.1227138540603374Illumina Paired End PCR Primer 2 (95% over 22bp)
AAATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG90170.12234750354511965No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC89730.12175048789069076No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAA89700.12170978227788881No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAAAAAAAAAAAAAAAA89110.12090923855945007No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAAAAAAAAAAAAA89110.12090923855945007No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA88520.12010869484101133Illumina PCR Primer Index 1 (95% over 22bp)
AGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAA86920.1179377288249063No Hit
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA86880.11788345467450367No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA86120.11685224581685377No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA85810.11643162115123343No Hit
AGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA85340.11579389988400257No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAAAAAAAAAA81680.1108278151221623No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA81170.1101358197045288No Hit
TTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG80870.10972876357650911Illumina Paired End PCR Primer 2 (95% over 22bp)
AGAAACCTCCCGTGGAGCAGAAGGGCAAAAAAAAAAAAAAA80210.1088332400948658No Hit
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG79720.10816838175243361Illumina Paired End PCR Primer 2 (95% over 22bp)
CCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA79300.10759850317320606No Hit
TCGACTGCATAATTTGTGGTAGTGGGTAAAAAAAAAAAAAA78480.1064858830899522No Hit
CCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA76980.10445060244985374No Hit
ACAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA74850.10156050394091391No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAA74090.10052929508326401No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)