FastQCFastQC Report
Mon 26 Jun 2023
SRR074372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8983577
Total Bases368.3 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA3514333.911949549717223No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA2786243.1014817371744017No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA2482812.7637209543592713No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA2432352.7075517914523357No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1158811.2899204849026173No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA711880.7924237750731139No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA625310.69605904196068No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA605430.6739297720718596No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA586280.6526130960974676No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA444710.4950255338157618No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA421520.4692117627532997No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAA409700.4560544201936489No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA380100.4231054066770953No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA365970.4073767052923351No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA348170.3875627714884617No Hit
AGAATTCACCAAGCGTTGGATTGTTCAAAAAAAAAAAAAAA343110.38193027120488865No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAAAAAAAAAA322010.35844296765085887No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA313380.34883654918302587No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA292970.32611731384948334No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA290480.32334558940163816No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA282870.31487457612930797No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAA278590.3101103268775901No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA275950.30717163107746503No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAA266850.2970420357058219No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA254910.2837511160643472No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT252060.28057866037103035No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA233760.2602081553928908No Hit
TAGTGACGAAAAATAACAATACAGGAAAAAAAAAAAAAAAA220990.24599332760213444No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAA219500.24433474550282142No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAA216130.24058345578826787No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA214690.23898053080638146No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT210680.23451682998876727No Hit
ACAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA201740.22456533739288928No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA194170.21613884981450038No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAA187380.20858061326796667No Hit
GCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA186070.20712239679138944No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA186010.2070556082504775No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT182970.20367165551093955No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA178370.1985512007076914No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA177440.19751597832355644No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA174560.19431012835978365No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA172550.1920727122392339No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAA169940.1891674107095648No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAAA167040.18593929789882138No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA165130.18381319601312485No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT161240.1794830722773345Illumina PCR Primer Index 1 (95% over 21bp)
AAAAAGCTCGTAGTTGGATCTTGGGAAAAAAAAAAAAAAAA161020.17923818096065744No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA160180.17830314138789036No Hit
TATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAA160030.17813617003561052No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAA157810.17566499402186903No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA157790.17564273117489837No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAA156890.17464090306121938No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA152460.16970968245722168No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAA151550.16869672292005733No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT148080.16483411897065056Illumina PCR Primer Index 2 (95% over 21bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA140770.15669704840288007No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA140670.15658573416802685No Hit
TACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAAAAAAAA140600.15650781420362958No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT140350.15622952861649653Illumina PCR Primer Index 2 (95% over 21bp)
AGAAACCTCCCGTGGAGCAGAAGGGCAAAAAAAAAAAAAAA139870.15569522028920105No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT139210.1549605463391698No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAA133200.1482705608244912No Hit
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA127990.14247108918863832No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCAAAAAAAAAAAAA123030.13694990313991856No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAA121760.13553621235728264No Hit
GGAACTACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAA121160.1348683269481633No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA120910.13459004136103023No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA120520.13415591584510267No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA119760.1333099276602182No Hit
TGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAAAA116390.12955863794566463No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAA116070.12920243239413431No Hit
TGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAA115430.1284900212910737No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAAA114980.1279891072342342No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA114470.12742140463648277No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAA112380.12509493712805045No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAA111760.12440478887196046No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT109730.12214510990444007Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA107920.12013032225359677No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT107460.11961827677327194No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAAAAAAAAAA106160.11817119172018006No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAA104740.11659052958526431No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT103540.11525475876702565No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA99600.11086897791380872No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAAAAAAAAAAAAA97430.1084534590174938No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA96780.10772991649094787No Hit
TAGAATTCACCAAGCGTTGGATTGTTCAAAAAAAAAAAAAA95360.10614925435603212No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAA94770.1054925003703981No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA94340.10501384916052926No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA94060.10470216930294025No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAAAAAAAAAAA91490.10184139346721245No Hit
CTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT90630.10088409104747474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)