FastQCFastQC Report
Mon 26 Jun 2023
SRR074373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8924915
Total Bases365.9 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA107599212.056047592610126No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA5532346.198759315915053No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA4418564.950814657618588No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA4369844.896225902431564No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA2176582.4387683244042098No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA1761091.9732288766895818No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA1648341.846897141317312No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA937501.0504301721641047No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA736720.8254644441991884No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA454810.5095958897087536No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA452900.5074558133046646No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA441260.494413672287075No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA430080.48188694234062734No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA396970.44478854980691696No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA374710.4198471357990524No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA353910.39654159171263814No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA349190.3912530259391826No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA334020.3742556651800045No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAA332440.37248534019651725No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA276280.30956037116319873No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA274530.3075995681751591No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA270420.30299448230039167No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA242900.2721594547399051No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA227120.25447861408203887No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA226710.2540192259534124No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAA219870.24635528741730311No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA213990.23976698937748986No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA191960.21508328090519632No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA181400.20325123544593982No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAA178670.20019238278459794No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA177190.19853410368614158No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAA172240.1929878323771151No Hit
ATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAAAA172130.19286458190358116No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA167500.18767685742665335No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA163620.18332947708745684No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA159950.1792173931068251No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA159170.1783434352035846No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA155240.17394003192187263No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAA154980.17364871262079246No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA150060.16813605507727525No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA147350.16509961159293954No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA144540.161951122223573No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA136690.15315552024865223No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAA136530.15297624683260289No Hit
TGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAA134640.15085857960552004No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGAAAAAAAAAAAA129360.1449425568758918No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAAAAAAA127940.14335150530845392No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT124380.13936267180135609No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA117760.13194523421231463No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAA116750.13081357077350317No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA116260.13026454593685205No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA115970.12993961287026262No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA115650.12958106603816394No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT111070.12444936450375157Illumina PCR Primer Index 2 (95% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA108260.12130087513438505No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA103730.11622519654248809No Hit
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA103310.11575460382535856No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAA103120.11554171664379996No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA101800.1140627109613929No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAA98240.11007387745429507No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA97820.10960328473716557No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA97700.10946882967512855No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAA97550.1093007608475823No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA96720.10837078000182634No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA94400.10577131546911092Illumina PCR Primer Index 1 (95% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAA93550.1048189254463488No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCAAAAAAAAA92540.10368726200753733No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA90050.10089731947026948No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)