FastQCFastQC Report
Mon 26 Jun 2023
SRR074374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10814176
Total Bases443.3 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA8067717.460309504857328No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA5582955.162621729108164No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA5272804.875822254048759No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA3753553.470953311653149No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA2355222.1779005631127144No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA2323932.148966319763984No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA2189632.024777477266876No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1805271.6693551131403817No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA1652261.5278649062119942No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA1521691.4071252400552756No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA1292311.1950147658037007No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA1130401.0452946206904714No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA973120.8998558928576712No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA824490.7624159251708128No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA750790.6942646393030777No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA748080.6917586693614012No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA693300.641102937477622Illumina PCR Primer Index 1 (95% over 22bp)
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAA665590.6154791636459404No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA645220.596642777036364No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA612340.5662382413602294No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA582170.5383396756257712No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA556480.5145838203484019No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA520970.4817472917030387No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAA480910.44470332274969443No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA451840.41782194038639653No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA440230.40708603225987816No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA438610.4055879985678058No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAA432930.40033563352399665No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAA428630.3963593712549157No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA402530.3722243839937504No Hit
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA391900.3623946937797203No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAA389230.35992571232426773No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA387420.3582519833226313No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA380230.3516033029238658No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAA374030.3458700875591446No Hit
TGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAA364940.3374644540647387No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA359740.33265595085561767No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA341820.316085109027262No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA338490.31300581754911333No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA332810.30775345250530417No Hit
AAAAAGCTCGTAGTTGGATCTTGGGAAAAAAAAAAAAAAAA326630.30203873138369486No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA316720.29287483392169683No Hit
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA293030.270968402955528No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA288360.26664999718887505No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA288210.266511290365535No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA286620.2650409980381307No Hit
TATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAA277850.2569312724335169No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA269930.24960755216116326No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAA262100.24236705598281366No Hit
AAAAAGCTCGTAGTTGGATCTTGGAAAAAAAAAAAAAAAAA251480.23254661289033948No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAAA249730.23092836661803914No Hit
AAATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAAAAA247310.22869056320148662No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAA246800.22821896000213052No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAA246540.2279785348416745No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA245880.22736822481897834No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA243900.22553729475088993No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAA237780.21987805635861668No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAA230110.2127855141251631No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA230060.2127392785173831No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAA229120.21187004909111892No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA226870.20978944674101846No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA221830.20512889747679344No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA221790.2050919089905694No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAA220710.20409321986252119No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA218970.20248422071177685No Hit
AGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA218610.20215132433576075No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA212600.19659380428060352No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA210180.19435600086405103No Hit
TCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAA206620.19106402559011432No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA204810.18939029658847792No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAA202870.18759635500661354No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA201490.18632025223188525No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA200100.18503490233560096No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA198720.18375879956087268No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAA196700.18189088100656028No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAAAAAAAAAA193290.1787376125559636No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCAAAAAAAAAAAAA191830.1773875328087873No Hit
AAAAATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAAA190000.1756953095640389No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA188840.17462264346354267No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA187740.17360546009238245No Hit
AATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAAA184080.1702210136028857No Hit
CTAGACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA179700.1661707743613568No Hit
CTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAAA177570.16420113746992837No Hit
GCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAA168090.1554348662348384No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA163550.1512366730484135No Hit
AAGATTAAGCCATGCATGTCTAAGTACGCAAAAAAAAAAAA162130.1499235817874612No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAA161540.14937800161565706No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA157840.1459565666399363No Hit
ATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAAAAA155830.14409789520717992No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAAAAAAAAAAA154830.14317318305157972No Hit
AATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAAAAAA154350.1427293212168916No Hit
AGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA153860.14227621226064752No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAA152950.14143472419905131No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA152930.14141622995593933No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA149170.13793931225088255No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAAA146840.13578473292833407No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAA145620.1346565840985018No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAA145610.13464733697694584No Hit
AGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAA138100.1277027486883883No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAA138030.12763801883749626No Hit
TGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAAAA137580.12722189836747616No Hit
CGTGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA137500.12714792139502815No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAAAAAAAA136910.12660234122322403No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA134730.12458646872401558No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCGAAAAAAAAAAAAA134700.12455872735934759No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAAAAAAAAAAAAA133830.1237542277839754No Hit
ACGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA133390.12334735443551131No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA133090.12306994078883125No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAAAAAAAAA132660.12267231456192317No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA132140.12219146424101104No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA129220.11949130474665846No Hit
TGGGCCGCCGGTGAAATACCACTACAAAAAAAAAAAAAAAA127070.11750317361211803No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAAA126800.11725350133010597No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA126020.1165322258487378No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAA121190.11206586613718882No Hit
TAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAAA120860.11176071112584075No Hit
TAAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAA119540.11054009108044849No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAAAAAAA119310.11032740728466044No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAA119180.11020719470443241No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA119030.11006848788109237No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGGCTGAAAAAAAAA117120.10830228766389599No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA116690.10790466143698789No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAA116500.10772896612742386No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAA116170.10742381111607578No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAA115290.10661006441914761No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAA113970.10538944437375533No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA112880.10438150812415112No Hit
AAGCCGCCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAA112820.1043260253948151No Hit
TGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAA112730.10424280130081108No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAA112270.10381743370923499No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA112160.10371571537211896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)