FastQCFastQC Report
Mon 26 Jun 2023
SRR074375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7583662
Total Bases310.9 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA3849435.07595143348952No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA2853653.7628918588407556No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA2808703.703619702460368No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAA2734963.6063843562648232No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA2420263.1914133303936802No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA2238272.951436917942809No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA2015142.6572123071940705No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA1953562.576011430889193No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA1928202.542571121972472No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA1850662.4403250039360933No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA1523932.009490929316206No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1457431.9218024221016177No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1114631.4697780570916796No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA875421.1543499697112027No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA637420.8405174175747812No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA612100.8071298536248056No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA599930.7910821974924515No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAA585830.7724895967146216No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA516580.6811748730362719No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAA469180.6186720874427156No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA437610.5770431224387373No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA427400.5635799696769186No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA421150.555338568622916No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA402130.530258336935375No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAA399510.526803541613537No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAA391420.5161358720892361No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA365850.48241865209710033No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA344460.4542132811298815No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAA324810.42830231621609716No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA305990.40348580936228434No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAA300130.39575867173405144No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAA285050.37587381927095376No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAAA279730.3688587386937867No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA264950.3493694734812812No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAA260070.3429345875383159No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA249780.3293659448430059No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA249140.3285220253750761Illumina PCR Primer Index 1 (95% over 22bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAA227940.300567192999899No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAA226780.29903758896427607No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA226780.29903758896427607No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA222850.2938553959815192No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAA210620.2777286223990468No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA201660.26591374984802857No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAA194820.2568943605345281No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA173710.22905820433452864No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA163830.21603019754836122No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAA162540.21432917237081506No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAA161560.21303692068554742No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA159280.21003045758104724No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA156770.20672071091775976No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAA156240.20602184010838037No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAA156090.20582404648308428No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA150560.19853205483050274No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA146360.19299383332221293No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA142960.1885105111488355No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA132320.17448034999450135No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAA130340.17186947414059328No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA129130.17027393889653838No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA126950.16739933820890224No Hit
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA125180.1650653734304087No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA122970.16215121401771335No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA121800.16060842374040404No Hit
TGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAA118280.15596686666678974No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA112620.14850345387228492No Hit
TATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAA110720.14599806795186812No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAA108760.14341356458133286No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAA107580.14185758806233717No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA107250.14142244208668583No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAA104250.13746656958076453No Hit
ACGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA102130.13467108634324684No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAA102040.13455241016806918No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAA101920.13439417526783234No Hit
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA101890.13435461654277314No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAAA101130.13335246217460642No Hit
AAAAATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAAA100060.13194153431416114No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA98660.13009546047806456No Hit
AAATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAAAAA97030.12794610308318066No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA96460.1271944873070556No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA96200.12685164502320911No Hit
TAAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAA95790.12631100911406654No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA95180.12550664837119588No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA93960.12389792688545455No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA90820.11975744699592361No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA90200.11893990001136655No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA90190.11892671376968014No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA89700.11828058792704632No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAA89660.11822784296030071No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAA87460.11532686978929177No Hit
ACAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA86020.11342805098644955No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAA85610.11288741507730699No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAAAAAAAAAA85190.112333592926478No Hit
TGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAAAA84340.11121276238313363No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAA83400.10997325566461164No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA82660.10899747377981772No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAA82300.10852276907910717No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAA81540.10752061471094043No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAA81520.10749424222756764No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA81180.10704591001022988No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA80890.10666350900132417No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAAAAAAAA78730.10381528079706084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)