FastQCFastQC Report
Mon 26 Jun 2023
SRR074376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR074376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8419537
Total Bases345.2 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAA110796413.159440952631957No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA4771825.6675563038680155No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAA2549543.0281237554986693No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA2430172.8863463632263864No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA2210982.6260113828111926No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA2206902.6211655106450626No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA2168392.575426653508382No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAA1552021.8433555194305815No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA1416941.6829191438911664No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA1096171.301936199104535No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAA1006781.1957664655431766No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA928631.1029466347139991No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAA923371.0966992603037435No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA897651.0661512622368665No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA773890.9191598065309291No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAAAAAAAAAA769850.9143614429154477No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAA726770.8631947338671949No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA624720.7419885440256394No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA619080.7352898383842247No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA544810.6470783369679354No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA500660.5946407741898396No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAAAAAAAAAAAAA498770.5923959951717059No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAA497920.5913864384704289No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAA481210.5715397414370885No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAA468090.5559569368244358No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAAAAAAAAAAA434970.5166198568876175No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA431860.5129260670747097No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAAAAAAAA418210.49671377416596657No Hit
AGAATTCACCAAGCGTTGGATTGTTCAAAAAAAAAAAAAAA416450.49462339793744003No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAA387270.45996591023948225No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA374810.4451669967125271No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAA320800.38101857619961765No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAA316660.3761014412075153No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA315390.37459304472443083No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA292770.3477269593327994No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAA281400.33422265381101124No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAA274540.3260749373748224No Hit
TGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAAAA268950.31943561742171805No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA260890.3098626444660793No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA259100.3077366368245665No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA238960.28381608157313165No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAAA235410.27959969770309223No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA224270.2663685663475319No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA215120.2555009853867261No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA207690.24667627210379858No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAAAAA194710.23125974741841507No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAAAAAAAAA189170.22467981315362115No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAA176490.2096196025981001No Hit
AAGCCATGCATGTCTAAGTACGCACGGAAAAAAAAAAAAAA175680.2086575544474714No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAAAAAAAAAAAAA174770.20757673492022183No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAA172960.20542697300338486No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAA171870.20413236499821782No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA169560.20138874619827668No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAAAAAA162810.19337167827637078No Hit
AATCCGGTAAAGCGAATGATTAGAGGTAAAAAAAAAAAAAA152210.18078191235456298No Hit
TGTACAGTGAAACTGCGAATGGCTCATTAAATCAAAAAAAA150450.1786915361260364No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA150090.17826395917020138No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAA149590.17767010228709726No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAAAAA144190.1712564479495725No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAAA139290.16543665049515194No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAAA133980.15912989039658593No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCAAAAAAA132370.1572176712329906No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA129090.15332197007982745No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAA126980.15081589403312795No Hit
AGATACCCGTCGCCGCGCTCTCTCTCTCCCCAAAAAAAAAA124750.14816729233448347No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAAAAA123530.14671828153970937No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAA123150.14626695030855022No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA122060.14497234230338318No Hit
AATCCGGTAAAGCGAATGATTAGAGGTCAAAAAAAAAAAAA121530.1443428540072928No Hit
GAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAAAAAAA121390.14417657408002366No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAA120580.14321452592939493No Hit
TAAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAA120300.14288196607485662No Hit
TAGCAGCCGACTTAGAACTGGTGCGGACCAAAAAAAAAAAA118760.14105288687489584No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA117780.13988892738401174No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAAAAAAAA115010.13659896025161478No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAA110030.1306841456958975No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAA109810.1304228486673317No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAAA105920.1258026421167815No Hit
TTAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAA100330.11916332216367717No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA100290.11911581361302884No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA100250.11906830506238052No Hit
GAGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAAAAAAAAA98380.116847280319571No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA98080.11649096618970853Illumina PCR Primer Index 1 (95% over 22bp)
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAA97330.1156001808650523No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAA97200.11544577807544525No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAA95370.11327226188328407No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCAAAAAAAAAAAAAA94750.11253587934823495No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAA92990.11044550311970835No Hit
AAGCCGCCGTGGCGCAATGAAGGTGAAGGGCCAAAAAAAAA90630.1076424986314568No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA90280.10722679881328392No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA88610.10524331682371607No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAAAA87610.10405560305750779No Hit
AAGCCATGCATGTCTAAGTACGCACGAAAAAAAAAAAAAAA87240.10361614896401074No Hit
AGTACAGTGAAACTGCGAATGGCTCATTAAATCAAAAAAAA85990.10213150675625038No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAA85020.10097942440302834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)