FastQCFastQC Report
Tue 27 Jun 2023
SRR1002819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1002819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66929528
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA48275757.212922523523549Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT9406551.4054409587349845No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCT8558311.2787046697834175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCTGCTAGGCGGACTACTTGCGTGCCTAGATCGGAAGAGCACACGTCT7922921.1837704876687611Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG7746511.1574129134752003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC7129491.065223409314944No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTG6046540.9034188915839956Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGT5578570.8334990798082426No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGAGATCGGAAGAGCACACG5521350.8249497889780426No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTG5170000.7724542745916272Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA5049230.7544099220302285Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTC3883970.5803073943685961No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGAGATCGGAAGAGCACACGTCTG3354750.5012361658967623Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCTCTGCTAGGCGGACTACTTGCGTGCCAGATCGGAAGAGCACACGTCT3119570.4660977140015092Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGATTAATAGGGACGGTCGGGGGCATAGATCGGAAGAGCACACGTCTGA3105160.4639447031510517Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG2771850.414144561127041Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGTTAGTCGATCCTAAGAGATGGAGATCGGAAGAGCACACGTCTGA2751060.41103830883134274Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTGA2747830.410555711673329Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAGATCGGAAGAGCACACGTC2627920.3926398524728876No Hit
TAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG2424240.36220784344990453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCT2390010.3570935088620377Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTGGGTTAGTCGATCCTAAGAGATGGAGATCGGAAGAGCACACGTCT2251590.3364120541833195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGATTAATAGGGACGGTCGGGGGCATAGATCGGAAGAGCACACGTCTG2102080.31407363279179257Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGATTAATAGGGACGGTCGGGGGCAAGATCGGAAGAGCACACGTCTGA2046190.30572305843842196Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTCT2017590.3014499071321704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCT1939650.2898048227682108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTGGGTTAGTCGATCCTAAGAGATGGGAGATCGGAAGAGCACACGTC1925020.28761894152308976No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCTG1856950.2774485425924414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT1552090.23189914024195718No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGTAGATCGGAAGAGCACACGTC1532340.22894827526648626No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGAGATCGGAAGAGCACACGTCTG1498210.22384888176710283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC1465830.2190109573161789No Hit
ATGATTAATAGGGACGGTCGGGGGCAAGATCGGAAGAGCACACGTCTGAA1405690.21002538670226392Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGATTAATAGGGACGGTCGGGGGCAGATCGGAAGAGCACACGTCTGAA1385170.20695947534547085Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGATCGGAAGAGCACACGT1368540.2044747723306819No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAGATCGGAAGAGCACACGTCT1321900.19750624866202554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGTGAGAATCCCGTGTGGCGAGGAGTGAGATCGGAAGAGCACACGTC1313830.1963005028214154No Hit
AGCGGAGAATTTGGACAAACTTGGTCAAGATCGGAAGAGCACACGTCTGA1305450.19504843960650672Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTACATGGTATAACTGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGT1301840.1945090663122561No Hit
CGGGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTC1301050.19439103171323724No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAGATCGGAAGAGCACACGTCT1296520.19371420040493936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGGAGAATTTGGACAAACTTGGTCAAGATCGGAAGAGCACACGTCTG1235790.18464047736897232Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGAGATCGGAAGAGCACACGT1233400.18428338535421915No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACAC1195230.1785803718801065No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCAGATCGGAAGAGCACAC1191570.17803352804161415No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAAGATCGGAAGAGCACACGTC1190960.17794238740186544No Hit
TTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTGA1183160.17677698250016047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT1181890.17658723067642135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGAGAATCCCGTGTGGCGAGGAGTGAGATCGGAAGAGCACACGTCTGA1126530.16831584409201272Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTCCAGACACAATAAGGATTGACAGAAGATCGGAAGAGCACACGTCTG1090190.16288625253714623Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTGAGAATCCCGTGTGGCGAGGAGTGAGATCGGAAGAGCACACGTCT1052830.1573042618797491Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGATCGGAAGAGCACACG1047390.1564914666662523No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGTAGATCGGAAGAGCACACGT1031520.15412031592393718No Hit
GGGGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTC997020.14896564039716523No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT994690.14861751303550205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCT969290.14482247656071923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGATTAATAGGGACGGTCGGGGGCATCAGATCGGAAGAGCACACGTCTG954490.14261119546517645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTCT944760.14115742755574193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG937420.14006075166106058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATGTAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT930490.13902533422916116No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGAGATCGGAAGAGCACACGTCTGA912130.13628215038360947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGAGCATAGCTGTTGGGACCCGAAAAGATCGGAAGAGCACACGTCTGA908980.13581150609638246Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTC899400.134380149819673No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTC866050.1293972968104601No Hit
GGTTGCGGCCATATCTACCAGAAAGCACCAGATCGGAAGAGCACACGTCT857700.12814971592209645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTGAAC850790.1271172867078937Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGA843520.12603106957515076Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTCCAGACACAATAAGGATTGACAGAAGATCGGAAGAGCACACGTCTGA830650.12410815148733755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTGAA821240.12270219506104987Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAGATCGGAAGAGCACACGTCT807010.12057607816986249Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG758650.11335056777929167TruSeq Adapter, Index 3 (100% over 49bp)
CCGATATAGTGTAACGGCTATCACATCACGCAGATCGGAAGAGCACACGT749220.11194162313530734No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAGATCGGAAGAGCACACGTCTGA734060.10967655412122435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCCTTGTCTATGTTCCTTGGAACAGGACAGATCGGAAGAGCACACGTC730080.10908189879958514No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTC729130.10893995845899287No Hit
GGGGCCTTTCCTTCTGGCTAACCTTGAGTAGATCGGAAGAGCACACGTCT722990.1080225756261123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGAGATCGGAAGAGCACACGTC721330.10777455355728789No Hit
CGATCTTGTAGAGAACTTTTACTCTGAATAGATCGGAAGAGCACACGTCT710430.10614597491259761Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTAATGATTAATAGGGACGGTCGGGGGCATAGATCGGAAGAGCACACGTC695690.1039436584701449No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGTC690490.10316672186900826No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGATCGGAAGAGCACACG689080.10295605252139235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)