FastQCFastQC Report
Tue 27 Jun 2023
SRR1002820.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1002820.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90757381
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA912943410.059164223789137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG18049321.9887440339425397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCTGCTAGGCGGACTACTTGCGTGCCTAGATCGGAAGAGCACACGTCT17618351.9412580889701965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTG15873431.7489960403330722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCT13481481.485441718508823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTG12195021.3436945695909845Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGTTAGTCGATCCTAAGAGATGGAGATCGGAAGAGCACACGTCTGA10690351.1779041971253006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTGA10517691.1588798491221337Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGTTAGTCGATCCTAAGAGATGGGAGATCGGAAGAGCACACGTCTG9909291.0918439790588492Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA9628871.0609462165947694Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTGGGTTAGTCGATCCTAAGAGATGGAGATCGGAAGAGCACACGTCT7100220.7823297589426913Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTCTGCTAGGCGGACTACTTGCGTGCCAGATCGGAAGAGCACACGTCT6810800.7504403415960186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGGGTTAGTCGATCCTAAGAGATGGAGATCGGAAGAGCACACGTCTG6061940.6679280443317331Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC5839930.6434661220556817No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGT5437720.599149065352602No Hit
TTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTGA4573220.5038951046857555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTC4457290.4911214879591997No Hit
GACGTGGGTTAGTCGATCCTAAGAGATGGGAGATCGGAAGAGCACACGTC4325280.4765761145090777No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAGATCGGAAGAGCACACGTC4247530.46800931816223296No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAGATCGGAAGAGCACACGTCT3988930.43951576786906177Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTAAGATCGGAAGAGCACACGTC3919370.4318513774653766No Hit
CCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTGAAC3885950.4281690323346814Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGAGATCGGAAGAGCACACG3552740.3914546630648145No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT3469660.382300586659723No Hit
AAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG3434760.37845516939277923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG3324840.3663437577600438Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTGAA3253970.35853502647900337Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAGATCGGAAGAGCACACGTCT2833740.312232456333221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGATTAATAGGGACGGTCGGGGGCATAGATCGGAAGAGCACACGTCTGA2792620.3077016953585296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTGAACT2661490.2932532837191501Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCT2545460.28046864860500986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGCCTTTCCTTCTGGCTAACCTTGAGTAGATCGGAAGAGCACACGTC2507570.2762937815492935No Hit
TCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTGAAC2490510.2744140446274006Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTAGATCGGAAGAGCACACGTCT2412070.2657712214062237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAGATCGGAAGAGCACACGTCT2409830.2655244095243339Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGA2312970.2548519993101167Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGAGCATAGCTGTTGGGACCCGAAAAGATCGGAAGAGCACACGTCTGA2308740.25438592151529804Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTGAA2308490.2543583755463371Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCTG2269810.2500964632287042Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTCCAGACACAATAAGGATTGACAGAAGATCGGAAGAGCACACGTCTG2073370.2284519426579751Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGATTAATAGGGACGGTCGGGGGCAAGATCGGAAGAGCACACGTCTGAA2020190.22259236414060912Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGATTAATAGGGACGGTCGGGGGCAAGATCGGAAGAGCACACGTCTGA1948850.21473184643792223Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC1908700.21030796382279915No Hit
GGTGAGAATCCCGTGTGGCGAGGAGTGAGATCGGAAGAGCACACGTCTGA1871570.20621683651272396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTGAGAATCCCGTGTGGCGAGGAGTGAGATCGGAAGAGCACACGTCT1790800.1973172848608313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGATTAATAGGGACGGTCGGGGGCAGATCGGAAGAGCACACGTCTGAA1667270.18370627067786366Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT1648640.1816535450708962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGTGAGAATCCCGTGTGGCGAGGAGTGAGATCGGAAGAGCACACGTC1627950.1793738406796908No Hit
GTAAGAGCATAGCTGTTGGGACCCGAAAAGATCGGAAGAGCACACGTCTG1603900.17672391846565075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGCCTTGTTGTAGACGGCCTTGGAGATCGGAAGAGCACACGTCTGA1556690.17152213768707142Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGATTAATAGGGACGGTCGGGGGCATAGATCGGAAGAGCACACGTCTG1525650.16810203018088413Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA1523110.16782216313624124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGTCGGTTATGGCATCTGCTAGATCGGAAGAGCACACGTCTGAACT1467060.16164635689520393Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGGCTCCGGTGCGCTTGTGACGGCCAGATCGGAAGAGCACACGTCTGA1411990.15557853085249343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTCCAGACACAATAAGGATTGACAGAAGATCGGAAGAGCACACGTCTGA1393450.1535357217943519Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCCACGTTCTAGCATTCAAGGTCCAGATCGGAAGAGCACACGTCTGA1393140.15350156479284036Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAAGATCGGAAGAGCACACGTC1313780.1447573724058873No Hit
GGGGCCTTTCCTTCTGGCTAACCTTGAGTAGATCGGAAGAGCACACGTCT1308930.14422298060804553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTGCGGCCATATCTACCAGAAAGCACCAGATCGGAAGAGCACACGTCT1286560.1417581673054228Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTATGCCGACTAGGGATCGGGTGGTGTAGATCGGAAGAGCACACGTCTG1265130.13939692684609312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGAGAATTTGGACAAACTTGGTCAAGATCGGAAGAGCACACGTCTGA1255400.13832483773413426Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTGGCTCCGGTGCGCTTGTGACGGCAGATCGGAAGAGCACACGTCTG1230810.1356154162271386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCAGATCGGAAGAGCACACGTC1195340.13170719415096385No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGAGATCGGAAGAGCACACGT1160830.12790474859559905No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCT1160300.12784635114140191Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCACGTTCTAGCATTCAAGGTCCAGATCGGAAGAGCACACGTCTGAA1155770.12734721818383016Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATCTTGTAGAGAACTTTTACTCTGAATAGATCGGAAGAGCACACGTCT1155370.12730314463349268Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGTAAGAGCCTGACCGAGTAGTGTAGATCGGAAGAGCACACGTCTG1122350.12366487305313494Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCTGGCTCCGGTGCGCTTGTGACGGAGATCGGAAGAGCACACGTCTGA1116330.12300156612055609Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTGAACT1092650.12039241194057813Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTGGTAAGAGCCTGACCGAGTAGTGTAGATCGGAAGAGCACACGTCT1089600.12005635111925496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCACTAATAGGGAACGTGAGCTGGGTTTAAGATCGGAAGAGCACACGT1076560.11861955337825361No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCAGATCGGAAGAGCACACGTCTG1070620.11796506115574225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCGGAGAATTTGGACAAACTTGGTCAAGATCGGAAGAGCACACGTCTG1061970.11701197062969457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCTGGTAAGAGCCTGACCGAGTAGTGAGATCGGAAGAGCACACGTCTG1025620.11300678674277743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGATTAATAGGGACGGTCGGGGGCAGATCGGAAGAGCACACGTCTGAAC1002930.11050671459988473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCACTAATAGGGAACGTGAGCTGGGTTTAGATCGGAAGAGCACACGTC985870.10862697767799183No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTC954920.10521678672063048No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGATCGGAAGAGCACACGT921750.10156198755889617No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)