FastQCFastQC Report
Tue 18 Jul 2023
SRR10100140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10100140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19043505
Total Bases440.5 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATACCTCATAGTATTATA678651735.636911377396125No Hit
TAATACCTCATAGTATTATA9466364.970912654997071No Hit
TGCTGTTACGGCAGTGCTG7846494.120297182687746No Hit
TTTAATACCTCATAGTATTATA7704244.045599798986584No Hit
ATTAATACCTCATAGTATTATA5443392.8583971280496945No Hit
AATACCTCATAGTATTATA4757642.4983006017012097No Hit
AAATACCTCATAGTATTATA3967882.083587028753373No Hit
AACTGGTGAAATACGATAGTACTCG2797171.4688314992434428No Hit
ACTGGTGAAATACGATAGTACTCG2024291.0629818407903378No Hit
TGGTGAAATACGATAGTACTCG1840470.9664554923056444No Hit
TTGGTGAAATACGATAGTACTCG1446110.7593717648090517No Hit
TGTGCTGTTACGGCAGTGCTG1385800.7277021745734307No Hit
TGTTGTTAGTTGCCAGC1175290.6171605489640694No Hit
TATTAATACCTCATAGTATTATA1038330.54524101524378No Hit
TCGGGAACTCTAACAAGACTGCC970010.5093652665305047No Hit
TACTGGTGAAATACGATAGTACTCG889420.467046376179175No Hit
CTGTTGTTAGTTGCCAGC795270.41760694788065533No Hit
TCTGAGGCCTAACCCCGCTA762910.4006142776762996No Hit
GTTGTTAGTTGCCAGC713510.3746736748303424No Hit
TTGCTGTTACGGCAGTGCTG620290.32572260201050174No Hit
GCTGTTACGGCAGTGCTG615490.32320205760441684No Hit
AGGTGCGGCTGGAAC610750.320713020003408No Hit
TCGGGAACTCTAACAAGACTGCCA575200.3020452379958416No Hit
CTAACAAGACTGCCA550690.2891747081222706No Hit
TTAATACCTCATAGTATTATAG507040.2662535074294359No Hit
ATACCTCATAGTATTATA497870.26143821738697787No Hit
CGGGAACTCTAACAAGACTGCC451270.2369679321112369No Hit
ACCTCATAGTATTATA447440.23495674772054828No Hit
TCTGGTGAAATACGATAGTACTCG429100.2253261676356322No Hit
TTAATACTTCATAGTATTATA417830.21940813941551202No Hit
CTTAATACCTCATAGTATTATA396570.20824422815022758No Hit
AACTGACGGAATAGTTCGTTGA385510.20243647374787363No Hit
TTAATACCTTATAGTATTATA379890.19948533633908253No Hit
TGTAGTAAAATGCGCAGGA369900.19423945329391834No Hit
AACTGACGGAATAGTTCGTTG362800.1905111480265844No Hit
GGTGAAATACGATAGTACTCG346750.182083077668738No Hit
GGGAACTCTAACAAGACTGCC346260.18182577209395015No Hit
GCCTAAGGGCTTCCAAGAAAAACCTC314400.16509565859856157No Hit
CGGGAACTCTAACAAGACTGCCA303690.1594716938924846No Hit
TTAATACCTCATAGTATTATAGA281970.14806623045495038No Hit
CCTGTTGTTAGTTGCCAGC281150.14763563745224423No Hit
GGGAACTCTAACAAGACTGCCA279990.14702650588744037No Hit
CTGGTGAAATACGATAGTACTCG278640.14631760277322897No Hit
GTTACGGCAGTGCTG264770.13903427966647947No Hit
GTGCTGTTACGGCAGTGCTG245900.12912538947005817No Hit
GGAACTCTAACAAGACTGCCA244140.1282011898544937No Hit
TGAACTCTAACAAGACTGCC231970.12181055955823258No Hit
TGAACTCTAACAAGACTGCCA229240.12037699992727179No Hit
CACTGGTGAAATACGATAGTACTCG217130.11401787643608673No Hit
ACTGACCGATTTGGAAACAGAT212480.11157609904269199No Hit
TTAATACCTCATAGTATTATG206940.10866697070733564No Hit
TACTGACGGAATAGTTCGTTG206340.10835190265657503No Hit
TTGTTGTTAGTTGCCAGC204960.10762724613982563No Hit
CATTAATACCTCATAGTATTATA204410.10733843375996173No Hit
TTGGATTAATACCTCATAGTATTATA199860.10494916770836041No Hit
ATCTGAGGCCTAACCCCGCTA198330.10414574417892085No Hit
GTAATACCTCATAGTATTATA197200.10355236601665503No Hit
TAACTGGTGAAATACGATAGTACTCG196580.10322679569753572No Hit
CGTGCTGTTACGGCAGTGCTG192900.1012943783195373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)