FastQCFastQC Report
Tue 18 Jul 2023
SRR10100141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10100141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14410982
Total Bases335.6 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATACCTCATAGTATTATA380118126.37697417150337No Hit
TAATACCTCATAGTATTATA5934364.117942829988962No Hit
TGCTGTTACGGCAGTGCTG4348993.01783042959876No Hit
AACTGGTGAAATACGATAGTACTCG3584512.4873461086829476No Hit
AATACCTCATAGTATTATA3352662.326461860822531No Hit
ATTAATACCTCATAGTATTATA2606341.8085790406233246No Hit
AAATACCTCATAGTATTATA2534071.7584297863948481No Hit
TTTAATACCTCATAGTATTATA2447451.698322848505397No Hit
ACTGGTGAAATACGATAGTACTCG1749271.2138451078490002No Hit
TGTTGTTAGTTGCCAGC1423200.9875801662926234No Hit
TCGGGAACTCTAACAAGACTGCC1306950.906912519910163No Hit
GCCTAAGGGCTTCCAAGAAAAACCTC1172380.8135323463730646No Hit
TGGTGAAATACGATAGTACTCG1139140.7904666038719638No Hit
TCTGAGGCCTAACCCCGCTA1049640.7283611900979406No Hit
CTGTTGTTAGTTGCCAGC991380.6879336883496212No Hit
TACTGGTGAAATACGATAGTACTCG895180.6211790424830175No Hit
GTTGTTAGTTGCCAGC895030.6210749551973627No Hit
TGTGCTGTTACGGCAGTGCTG814180.5649719082294322No Hit
TTGGTGAAATACGATAGTACTCG682880.47386083751960834No Hit
TCGGGAACTCTAACAAGACTGCCA635620.44106640338597325No Hit
TTAATACCTCATAGTATTATAG631440.4381658376923932No Hit
CGGGAACTCTAACAAGACTGCC623280.4325034893527728No Hit
CTAACAAGACTGCCA598820.4155303226386654No Hit
GGGAACTCTAACAAGACTGCC583580.40495505441613905No Hit
AGGTGCGGCTGGAAC553140.38383227458059416No Hit
TATTAATACCTCATAGTATTATA473500.32856886505027905No Hit
AACTGACGGAATAGTTCGTTG455770.31626574788588313No Hit
TTAATACCTCATAGTATTATAGA445340.3090282119566869No Hit
TAACTGGTGAAATACGATAGTACTCG409820.28438034271363327No Hit
AACTGACGGAATAGTTCGTTGA404460.28066095703956884No Hit
TTGCTGTTACGGCAGTGCTG374750.26004473532754396No Hit
GGGAACTCTAACAAGACTGCCA355600.24675625852561608No Hit
CCTGTTGTTAGTTGCCAGC349520.24253725388040873No Hit
GCTGTTACGGCAGTGCTG348040.2415102593286148No Hit
ATACCTCATAGTATTATA347190.240920431376571No Hit
TTAATACTTCATAGTATTATA339490.2355772840462919No Hit
TTGTTAGTTGCCAGC337360.23409924458999395No Hit
GGAACTCTAACAAGACTGCCA333000.2310737741536281No Hit
CGGGAACTCTAACAAGACTGCCA327760.22743765830808754No Hit
ACCTCATAGTATTATA323040.22416237838615025No Hit
TGCGCCTAAGGGCTTCCAAGAAAAACCTC322850.2240305344909875No Hit
TTAATACCTTATAGTATTATA303510.2106102137938969No Hit
TGAACTCTAACAAGACTGCCA284960.19773808613458818No Hit
TGAGCGCAACCCCTGTTGTTAGTTGCCAGC277550.19259617422324168No Hit
GGAACTCTAACAAGACTGCC271920.18868943143499867No Hit
TCTGGTGAAATACGATAGTACTCG270420.18764855857845078No Hit
GGTGAAATACGATAGTACTCG263140.18259685564800512No Hit
CCGGGAACTCTAACAAGACTGCC262960.18247195090521937No Hit
TGAACTCTAACAAGACTGCC258600.17944648046885353No Hit
GAGGCCTAACCCCGCTA256050.17767699661272215No Hit
AAACTGGTGAAATACGATAGTACTCG250050.17351350518653066No Hit
ATCTGAGGCCTAACCCCGCTA248610.1725142672442447No Hit
TTAAGGTGGTTATTGCGGCG245620.17043946068352595No Hit
TTGTTGTTAGTTGCCAGC232870.161592041402869No Hit
CACTGGTGAAATACGATAGTACTCG226420.15711628811971315No Hit
TGGGGATGACGTCAAATCATCACGGCCC220860.15325811939810902No Hit
CTGGTGAAATACGATAGTACTCG215590.1496011860954375No Hit
GCCTAAGGGCTTCCAAGAAAAACCTCT214880.1491085062766715No Hit
TTGGGGATGACGTCAAATCATCACGGCCC207500.14398741182245595No Hit
CTTAATACCTCATAGTATTATA203560.14125338578592353No Hit
TACTGACGGAATAGTTCGTTG197360.13695111131219231No Hit
GTTACGGCAGTGCTG185130.12846452795513866No Hit
TGTTGTTAGTTGCCAGCG174650.12119229626405752No Hit
TACTGACGGAATAGTTCGTTGA174220.12089391271184712No Hit
TCATAGTATTATAGA173130.12013754510275566No Hit
GTGCTGTTACGGCAGTGCTG169550.11765332855179474No Hit
TTGGATTAATACCTCATAGTATTATA167170.11600181028607211No Hit
TTAATACCTCATAGTATTAT155300.10776503641458993No Hit
ACTGACGGAATAGTTCGTTG152810.10603718747272045No Hit
GCCTAAGGGCTTCCAAGAAAAACCT149710.10388605023585486No Hit
TCTGAGGCCTAACCCCGCT148170.10281742076979902No Hit
GCCTAAGGGCTTCCAAGAAAAACCTCTA145290.10081894488522712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)