FastQCFastQC Report
Tue 18 Jul 2023
SRR10100142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10100142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26216828
Total Bases637.2 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATACCTCATAGTATTATA449410317.14205471386546No Hit
TGAGCGCAACCCCTGTTGTTAGTTGCCAGC11073434.223787103458894No Hit
TGCTGTTACGGCAGTGCTG10765264.1062404650936415No Hit
TAATACCTCATAGTATTATA7531822.8728952259213054No Hit
AACTGGTGAAATACGATAGTACTCG6920652.639773965027348No Hit
TTTAATACCTCATAGTATTATA5061111.9304814449711458No Hit
ATTAATACCTCATAGTATTATA4812301.835576752458383No Hit
AATACCTCATAGTATTATA4586171.7493229920873723No Hit
AAATACCTCATAGTATTATA3803171.4506598586221033No Hit
TGGTGAAATACGATAGTACTCG3345821.276210836795359No Hit
ACTGGTGAAATACGATAGTACTCG3229131.2317012569178851No Hit
TGTTGTTAGTTGCCAGC2938711.1209250791133085No Hit
TTGGTGAAATACGATAGTACTCG2498560.9530367289284577No Hit
TCTGAGGCCTAACCCCGCTA2203610.8405326533019173No Hit
GCCTAAGGGCTTCCAAGAAAAACCTC2113950.8063332451965585No Hit
TGTGCTGTTACGGCAGTGCTG2066690.7883066555572628No Hit
TTGGGGATGACGTCAAATCATCACGGCCC2063540.7871051372042415No Hit
CTGTTGTTAGTTGCCAGC1978960.7548434158396279No Hit
TTGGGGATGACGTCAAATCATCACGGCCCT1952250.7446553030748037No Hit
TGAGCGCAACCCCTGTTGTTAGTTGCCAGCG1922400.7332694863009361No Hit
GTTGTTAGTTGCCAGC1790420.682927774481337No Hit
TACTGGTGAAATACGATAGTACTCG1611060.6145137008947078No Hit
CGAGCGCAACCCCTGTTGTTAGTTGCCAGC1559700.5949232302244956No Hit
TTGCTGTTACGGCAGTGCTG1077390.41095360582904994No Hit
GAGCGCAACCCCTGTTGTTAGTTGCCAGC977290.3727720226108208No Hit
TCGGGAACTCTAACAAGACTGCC910670.34736086303041697No Hit
CTAACAAGACTGCCA891930.3402127824159353No Hit
TATTAATACCTCATAGTATTATA886160.3380119059407187No Hit
GCTGTTACGGCAGTGCTG835970.3188677135159143No Hit
AGGTGCGGCTGGAAC799640.30501020184440314No Hit
TTAAGGTGGTTATTGCGGCG798650.3046325817905965No Hit
TGAGCGCAACCCCTGTTGTTAGTTGCCAG793880.30281313971316437No Hit
TAACTGGTGAAATACGATAGTACTCG774280.295337025516588No Hit
TTGTTAGTTGCCAGC724980.2765323097058119No Hit
ATCTGAGGCCTAACCCCGCTA704600.26875867667896364No Hit
GGGAACTCTAACAAGACTGCC681280.2598636265226289No Hit
GGTGAAATACGATAGTACTCG677590.25845613359480407No Hit
CCTGTTGTTAGTTGCCAGC638660.24360689248905323No Hit
AACTGACGGAATAGTTCGTTGA631460.24086056482500476No Hit
TTAATACCTCATAGTATTATAG628040.23955605918458178No Hit
GGAACTCTAACAAGACTGCCA618090.23576078692662591No Hit
ACGAGCGCAACCCCTGTTGTTAGTTGCCAGC608840.23223251874711923No Hit
TCTGGTGAAATACGATAGTACTCG589710.22493567871750159No Hit
GGGAACTCTAACAAGACTGCCA553660.2111849686773701No Hit
GCCTAAGGGCTTCCAAGAAAAACCTCT552500.21074250477594011No Hit
CTGGTGAAATACGATAGTACTCG539440.20576097154087442No Hit
TTGTTGTTAGTTGCCAGC531280.20264846685495286No Hit
TGCGCCTAAGGGCTTCCAAGAAAAACCTC526740.20091675468901118No Hit
GAGGCCTAACCCCGCTA521020.19873494993368382No Hit
ATACCTCATAGTATTATA517300.19731601397392542No Hit
TCGCCAAAGAGGGTGATAGCCCCGTATGGG516170.19688499310442895No Hit
TGTTGTTAGTTGCCAGCG510970.19490153423594953No Hit
GGAGCGCAACCCCTGTTGTTAGTTGCCAGC498230.1900420600081749No Hit
TCGGGAACTCTAACAAGACTGCCA495960.18917620392520407No Hit
AAACTGGTGAAATACGATAGTACTCG473820.1807312463582551No Hit
GTGCTGTTACGGCAGTGCTG450470.17182475316998685No Hit
TGAACTCTAACAAGACTGCCA420290.16031306304485043No Hit
TTAATACTTCATAGTATTATA419850.16014523190982524No Hit
CACTGGTGAAATACGATAGTACTCG406350.1549958675397344No Hit
TTAATACCTTATAGTATTATA402800.1536417754275994No Hit
AACTGACGGAATAGTTCGTTG397280.15153625755182892No Hit
TGGGGATGACGTCAAATCATCACGGCCC390400.1489119888950715No Hit
TTCTGAGGCCTAACCCCGCTA390110.14880137291971401No Hit
TGCTGTTACGGCAGTGCTGGGGTTGTAGGACC364130.1388917072652725No Hit
TTGGGGTGACGGAGTGATGAAAGCGCCGCG361880.13803347987025738No Hit
ACCTCATAGTATTATA346400.13212887539255322No Hit
TTATCTGAGGCCTAACCCCGCTA343930.1311867324300255No Hit
GTTGTTAGTTGCCAGCG342930.13080529803224097No Hit
CTGTTGTTAGTTGCCAGCG333610.12725032944488934No Hit
CGGGAACTCTAACAAGACTGCC332480.1268193085753929No Hit
GCCTAAGGGCTTCCAAGAAAAACCTCTA316460.12070872952288506No Hit
TTGCGCCTAAGGGCTTCCAAGAAAAACCTC316250.12062862829935032No Hit
TGGGGATGACGTCAAATCATCACGGCCCT314590.11999544719902805No Hit
CGTGCTGTTACGGCAGTGCTG310500.11843538051208942No Hit
GTTACGGCAGTGCTG308690.11774498425209945No Hit
GCCTAAGGGCTTCCAAGAAAAACCTCTAA294610.1123743879312936No Hit
GCCTAAGGGCTTCCAAGAAAAACCT291280.11110421138667119No Hit
CTGAGGCCTAACCCCGCTA289960.11060071798159564No Hit
AGGTAGCCGTACCGGAAGGTGCGGCTGGAAC284210.10840747019433472No Hit
CGAGCGCAACCCCTGTTGTTAGTTGCCAGCG279660.10667194368441522No Hit
TGAGGCCTAACCCCGCTA269080.10263636775585513No Hit
TTGGATTAATACCTCATAGTATTATA267620.10207947353508975No Hit
TACTGACGGAATAGTTCGTTGA266480.10164463832161542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)