FastQCFastQC Report
Mon 3 Jul 2023
SRR10103857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10103857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53774668
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length12-36
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC30528805.677171265845844No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC22369474.159852739583627No Hit
TTTTGGTGACTCT18414753.4244283944254197No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC15908222.9583111512654994No Hit
TTTGGTGACTCT14732162.7396096615603467No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT8470231.5751338529881764No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG7944151.4773034024124518No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG7913611.471624148381539No Hit
TTTTGGTGACTCTAGAT6116331.1373998627011515No Hit
TCGCTTCTGGCGCCAAGCGCCCGG5463291.015959782401632No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC5085510.9457073728470067No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4593370.8541884442689632No Hit
TTTGGTGACTCTAGAT4554740.8470047643994753No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC4268250.7937287497525788No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3294370.6126248887301359No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT2826100.5255448532011393No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2777650.5165350346746911No Hit
AGGAATAATGGAAT2274810.4230263216129944No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT2226140.41397559162987296No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2099220.39037340035274604No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2021700.37595769071042895No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT1958790.36425887371354854No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC1842550.3426427476967408No Hit
TTTTGGTGACTCTAGATAACCTC1723200.3204482824515067No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1685550.31344684452538135No Hit
TTTGGTGACTCTAGATAACCTC1666760.30995263420315305No Hit
CTTTGGTGACTCT1660970.30887591904798No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCT1633450.30375826774049075No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1609270.29926172672976803No Hit
CCGGCCGAGGTGGGATCCCGAGGCC1576020.2930785179371075No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1506210.2800965688900208No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT1453990.27038567676512665No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1449140.26948376510664834No Hit
CCGCCGAGGGCGCACC1423070.2646357574908691No Hit
AGACGACCTGCTT1414810.26309971825395556No Hit
CCCCCGCGGGGGCGCGCCGGC1412270.26262737689054627No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG1345360.2501847152268797No Hit
TGGGGGGGCCGGGCCACCCCTCCC1343910.24991507153516967No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1337510.24872492006831173No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1325200.2464357381062771No Hit
CGCTTCTGGCGCCAAGCGCCCGG1318890.24526232314442184No Hit
AGATAACCTCGGGCCGATCGCAC1292500.2403548079552997No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC1275180.2371339605481153No Hit
CCGCCGAGGGCGCACCACCGGCCC1267900.2357801632545644No Hit
TTGGTGACTCTAGAT1243210.2311887820488264No Hit
AGCTAGGAATAATGGAAT1213920.22574197947628424No Hit
ATTGATCATCGAC1152740.2143648752977889No Hit
AACCTCGGGCCGATCGC1129360.2100171032204234No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC1111990.20678695775490424No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG1045760.19447074968459127No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1040840.1935558207444442No Hit
AACTGTGGTAATTCT1032300.1919677123808556No Hit
AGAATCCCGCCCAGGCGGAACGAT1021620.1899816471205364No Hit
TCGCTTCTGGCGCCAAGCGCCCG1018890.18947397313545478No Hit
TTGGTGACTCTAGATAACCTC1010810.1879714069085466No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC1006720.18721082573675768No Hit
AAGTCCTTCTGATC984390.18305831288442356No Hit
CGAGGGCGCACCACCGGCCCGTCTCG953540.1773214108918348No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC927780.1725310512377315No Hit
CCGCCGAGGGCGCACCACCGGCCCGT905110.16831531158872054No Hit
ACCCCTCCCACGGC899790.16732599818189486No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGT890140.1655314729232731No Hit
ACGGCGCGACCGCTC876910.16307120668787764No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG866500.16113535094256648No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC848230.1577378404270204No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT830540.1544481873881583No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT822800.15300884795792696No Hit
AAATTCTAGAGCTAATACATGCC820360.15255510271118736No Hit
CGTTTACTTTGAAAAAATT815970.1517387331893895No Hit
CCGCCGAGGGCGC802650.14926173044899135No Hit
ACTGACCCGGTGAGGCGGGG796100.1480436848071289No Hit
TCGCTTCTGGCGCC794010.1476550259687331No Hit
CGGCCGAGGTGGGATCCCGAGGC792320.1473407515970159No Hit
CGCTTCTGGCGCCAAGCGCCCGGC792150.14730913819867747No Hit
AACCTCGGGCCGATCGCAC790460.1469948638269603No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC780360.14511665604332508No Hit
ACCGGCCCGTCTCGC778480.14476704904993556No Hit
AAATTCTAGAGCT747460.13899853365900836No Hit
ACCGGCCCGTCTCG744480.13844436938225263No Hit
CCGGCCGAGGTGGGATCCCGAGGC738310.13729698898373488No Hit
AGAGCTAATACATGCC727640.1353127833350826No Hit
AGATAACCTCGGGCCGATCGC709590.13195618427620975No Hit
ACTTGGATAACTGTGGT705390.13117514737608424No Hit
TAGGAATAATGGAAT702730.13068049067267137No Hit
ACCGCAGCTAGGAAT699440.13006867843423972No Hit
GAGGGCGCACCACCGGCCCGTCTCG686300.12762514870384695No Hit
AGAGCTAATACATGC685270.12743360870214948No Hit
TTGGGGGGGCCGGGCCACCCCTCCC684010.12719929763211182No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG683950.12718813996211004No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC663650.12341312827816994No Hit
AACTGTGGTAATTCTAGAGCT653560.12153678010620168No Hit
ACTTGGATAACTGTGGTAATTCT642370.1194558746508672No Hit
ACTTTGAAAAAATT640760.11915647717248574No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGC636810.11842193056403436No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT625420.11630383287536056No Hit
ACCCCTCCCACGGCGCGACCGCTC622710.1157998781136129No Hit
ATCGACACTTCGAACGC602340.11201184914800402No Hit
ACGGCGCGACCGCT592960.11026753340439034No Hit
AGGAATAATGGAATA589870.10969291339929796No Hit
TAACTGTGGTAATTCT589590.10964084427262295No Hit
AAATTCTAGAGCTAATACATGC586240.10901787436418948No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC586050.10898254174251712No Hit
AAGCGCCCGGCC583540.10851577921410878No Hit
ACCCCTCCCACGGCGC571710.10631585861208849No Hit
TGCCGCCTGGAT565490.10515918015523591No Hit
TAACTGTGGTAATTCTAGAGCT563700.10482630966684907No Hit
TCGCTGCGATCT559910.10412151684506914No Hit
ACTTTGAAAAAATTAGAGTGTTC554110.1030429420782291No Hit
CTAAACCATTCGT550450.1023623242081197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)