FastQCFastQC Report
Mon 3 Jul 2023
SRR10103858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10103858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47445929
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length12-36
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC15971493.3662508747589284No Hit
TTTGGTGACTCT15449383.256207713837788No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC14925093.145705082516142No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC11646092.4546025856085567No Hit
TTTTGGTGACTCT9503962.003113902564749No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG5957011.2555365919803152No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG5769111.215933615716535No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5239301.1042675547569107No Hit
TTTGGTGACTCTAGAT4636610.9772408503161568No Hit
TTTTGGTGACTCTAGAT4628840.9756031966409595No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC4324350.9114269845996692No Hit
TCGCTTCTGGCGCCAAGCGCCCGG4295880.9054264697820544No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4045660.8526885415184936No Hit
AGCTAGGAATAATGGAAT3091680.6516217650622881No Hit
AGGAATAATGGAAT2848370.6003402315085874No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC2708990.5709636331496428No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2421030.5102713870351238No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2267390.47788926211140265No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG2091800.4408808182468089No Hit
TTTTGGTGACTCTAGATAACCTC2008790.4233851127669984No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1953940.41182458457078586No Hit
CCGGCCGAGGTGGGATCCCGAGGCC1906840.40189749472499525No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT1867100.3935216443965087No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1842340.3883030723247088No Hit
TTTGGTGACTCTAGATAACCTC1753560.36959124564722934No Hit
CTTTGGTGACTCT1611270.3396013175334811No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC1556880.32813774180710004No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1549540.3265907176145713No Hit
AGATAACCTCGGGCCGATCGCAC1545750.32579191356965526No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCT1478850.31169165219633493No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT1473110.3104818539858288No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1429340.3012566157151228No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG1419430.29916792228897027No Hit
TGGGGGGGCCGGGCCACCCCTCCC1405040.2961349961131544No Hit
CCCCCGCGGGGGCGCGCCGGC1357160.2860435086011278No Hit
CCGCCGAGGGCGCACC1325210.2793095272726138No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT1295450.2730371240069933No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1291820.27227204256027954No Hit
AACCTCGGGCCGATCGC1259260.2654094938261194No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC1252130.26390673054373115No Hit
TCGCTTCTGGCGCC1243210.2620266956939551No Hit
TTGGTGACTCTAGATAACCTC1209910.25500817994310954No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1205770.25413560771462607No Hit
GAGGGCGCACCACCGGCCCGTCTCG1169420.2464742549355499No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT1154630.2433570222642284No Hit
TTGGTGACTCTAGAT1116960.23541745804998357No Hit
CCGCCGAGGGCGCACCACCGGCCC1088640.2294485581681834No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT1072010.22594351561753592No Hit
AACCTCGGGCCGATCGCAC1029840.21705550332885248No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1024350.21589839667803742No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG998080.2103615675857037No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC992770.2092423988578662No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT962550.20287304312241414No Hit
TCGCTTCTGGCGCCAAGCGCCCG955650.20141875607494164No Hit
TAGGAATAATGGAAT888160.18719414262075046No Hit
AGGAATAATGGAATA846720.17845998968636487No Hit
AGAATCCCGCCCAGGCGGAACGAT831420.17523526623327365No Hit
AGACGACCTGCTT827650.17444067751313289No Hit
AGATAACCTCGGGCCGATCGC826290.17415403542841368No Hit
ACGGCGCGACCGCTC823190.17350066008824488No Hit
AAGTCCTTCTGATC809290.17057100936942346No Hit
AAGCGCCCGGCC809220.17055625573270997No Hit
ACCCCTCCCACGGC793380.16721771851068615No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG772770.1628738263297574No Hit
CCGCCGAGGGCGC761430.16048373718217215No Hit
CCGGCCGAGGTGGGATCCCGAGGC760540.16029615522967208No Hit
CGAGGGCGCACCACCGGCCCGTCTCG739810.15592697110009163No Hit
ACTGACCCGGTGAGGCGGGG719000.1515409256714101No Hit
CGCTTCTGGCGCCAAGCGCCCGG695860.1466637949064081No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG694720.146423521394217No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG687570.14491654278705343No Hit
CGGCCGAGGTGGGATCCCGAGGC657970.13867786211963518No Hit
CCGCCGAGGGCGCACCACCGGCCCGT655280.13811090093735967No Hit
AGCTAGGAATAATGGAATA650990.13720671377306154No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC638240.13451944422881887No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC627700.13229796807224492No Hit
TTCTGGCGCCAAGCGCCCGG620680.13081838907612073No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC615430.12971186632260906No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC601090.1266894784587314No Hit
ACCCCTCCCACGGCGCGACCGCTC599960.12645131260892795No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC592330.12484316620715763No Hit
CGGCCGAGGTGGGATCCCGAGGCCT588630.12406333112373034No Hit
ACCGGCCCGTCTCGC574090.12099879001209989No Hit
ACCCCTCCCACGGCG568510.11982271439979603No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC565690.11922835360648118No Hit
ACCCCTCCCACGGCGC557230.11744527122653664No Hit
AATTTTTGACAGTCTCTACGGAGACTG554410.11685091043322178No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG552440.11643570094285645No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGT549530.11582237118805282No Hit
CGCTTCTGGCGCCAAGCGCCCGGC541470.11412359530361393No Hit
ACCGGCCCGTCTCG541170.1140603654319847No Hit
AAGCGCCCGGCCG524410.11052792327029787No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT524210.11048577002254505No Hit
CGGCCGAGGTGGGATCCCGAGGCCTC515650.10868161101872406No Hit
TGCCGCCTGGAT503070.10603017173507132No Hit
ACCGCAGCTAGGAAT498120.10498687885318887No Hit
AAATTCTAGAGCTAATACATGC497170.10478665092636293No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC496810.10471077508040784No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC487170.10267898853872162No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG483940.10199821358751349No Hit
ACGGCGCGACCGCT476950.1005249575785522No Hit
AACTGTGGTAATTCTAGAGCT475240.10016454731026554No Hit
TTGGGGGGGCCGGGCCACCCCTCCC474510.10001068795596772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)