FastQCFastQC Report
Mon 3 Jul 2023
SRR10103859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10103859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80136121
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length12-36
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCT33682764.203193214206113No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC27014043.3710191687466384No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC26707773.332800448377081No Hit
TTTGGTGACTCT22302122.783029640279194No Hit
TTTTGGTGACTCTAGAT14723741.837341240911823No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC12820901.5998902667125603No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG9678541.2077624770482713No Hit
TTTGGTGACTCTAGAT8589111.0718150433061266No Hit
AGGAATAATGGAAT8191501.022198216956371No Hit
TCGCTTCTGGCGCCAAGCGCCCGG7106400.8867911138349209No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6287720.7846299423452253No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC6045210.7543676839561525No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG6015060.7506053356388438No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC5090550.6352378847985417No Hit
AGCTAGGAATAATGGAAT5071120.6328132603273872No Hit
TTTTGGTGACTCTAGATAACCTC4403770.5495362072741204No Hit
CTTTGGTGACTCT4062390.5069361917330638No Hit
AGATAACCTCGGGCCGATCGCAC3859450.48161178153357337No Hit
AGGAATAATGGAATA3767010.4700764091139375No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC3762970.4695722669181853No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3679380.4591412653976601No Hit
TTTGGTGACTCTAGATAACCTC3599190.4491345419626687No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG3457340.43143341065884633No Hit
CCGCCGAGGGCGCACC3363870.41976950693682813No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCT3295540.4112427652943172No Hit
CCGGCCGAGGTGGGATCCCGAGGCC3184520.39738883792491025No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2930140.36564534986663505No Hit
CCCCCGCGGGGGCGCGCCGGC2804890.3500156939215962No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2648590.3305113807542544No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT2603630.3249009270114285No Hit
TAGGAATAATGGAAT2592230.32347834754816746No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2396430.29904492132829835No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2382430.29729789391727607No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT2276040.28402173347022874No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT2187220.2729380924240144No Hit
TGGGGGGGCCGGGCCACCCCTCCC2174100.27130087816454207No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2156680.26912707691454146No Hit
CCGCCGAGGGCGC2107640.2630074894690747No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG2095790.2615287555533166No Hit
AACCTCGGGCCGATCGC2061920.2573021970953648No Hit
AACCTCGGGCCGATCGCAC1964940.24520028864386886No Hit
TTGGTGACTCTAGATAACCTC1940150.2421068022496372No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC1902440.23740105913037643No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1812870.22622382733000018No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1788320.22316028997710036No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC1693850.21137159858286625No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC1644480.20521083120556832No Hit
AGATAACCTCGGGCCGATCGC1619900.20214355022250205No Hit
ACCCCTCCCACGGC1595560.19910621828076752No Hit
TTGGTGACTCTAGAT1538670.1920070476084062No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT1497380.18685456462261257No Hit
TCGCTTCTGGCGCCAAGCGCCCG1457600.1818905110218649No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1440140.17971171826497567No Hit
CCGCCGAGGGCGCACCACCGGCCC1432970.1788169906551878No Hit
AGAATCCCGCCCAGGCGGAACGAT1428870.1783053611991027No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1391950.17369820034089245No Hit
AAGTCCTTCTGATC1391430.1736333107513402No Hit
CTTTGGTGACTCTAGAT1351910.16870170194536868No Hit
CGGCCGAGGTGGGATCCCGAGGCCT1347660.16817135433845118No Hit
ACCGCAGCTAGGAAT1345770.1679355056379632No Hit
GAGGGCGCACCACCGGCCCGTCTCG1332930.1663332319267113No Hit
AGACGACCTGCTT1294030.16147899147751363No Hit
AAATTCTAGAGCTAATACATGC1235100.1541252539538319No Hit
CCGCCGAGGGCGCACCACCGGCCCGT1222090.1525017663383033No Hit
ACGGCGCGACCGCTC1160330.14479487970225063No Hit
TCGCTTCTGGCGCC1148820.14335857359504586No Hit
CGCTTCTGGCGCCAAGCGCCCGG1140640.14233781043631996No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG1107490.13820109910236358No Hit
TGCCGCCTGGAT1078080.1345310936624946No Hit
AGCTAGGAATAATGGAATA1068050.13327947331016932No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1066860.13313097598023244No Hit
CCGGCCGAGGTGGGATCCCGAGGC1063130.1326655179628672No Hit
CGAGGGCGCACCACCGGCCCGTCTCG1062490.13258565385264903No Hit
CGGCCGAGGTGGGATCCCGAGGC1062260.13255695268803938No Hit
TTTTGGTGACTCTAGATAACCT1057400.13195048460107023No Hit
CCCCCGCGGGGGCGCGCCGG1054030.1315299501457027No Hit
AACTGTGGTAATTCTAGAGCT1040270.12981287177601222No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT1022130.1275492234020162No Hit
CGGCCGAGGTGGGATCCCGAGGCC1009030.12591450489598816No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1003290.12519822365746902No Hit
ACCGGCCCGTCTCG986790.12313922706590702No Hit
ACCGGCCCGTCTCGC986070.12304937994191158No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC965190.12044381334604404No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGCT951960.11879287244262796No Hit
TAACCTCGGGCC919410.11473103371200111No Hit
TAACTGTGGTAATTCTAGAGCT907030.11318616232996853No Hit
TTGGGGGGGCCGGGCCACCCCTCCC886370.11060804902198848No Hit
CGGCCGAGGTGGGATCCCGAGGCCTC886000.11056187758326859No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC885210.11046329532221806No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGT883730.11027860956733856No Hit
AAATTTTTGACAGTCTCTACGGAGACTG881610.11001405970224089No Hit
TTTGGTGACTCTAGATAACCT878750.10965716695970347No Hit
ACCCCTCCCACGGCGC873680.10902449346156898No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG873310.1089783220228491No Hit
AATTTTTGACAGTCTCTACGGAGACTG871600.10876493510335995No Hit
AGATAACCTCGGGC871240.10872001154136222No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG869890.10855154818387078No Hit
CGCTTCTGGCGCCAAGCGCCCGGC865570.10801246543989819No Hit
AGATAACCTCGGGCC864780.10791388317884765No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC862030.10757071708025398No Hit
ACTGACCCGGTGAGGCGGGG858660.10715018262488647No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT855210.10671966515574169No Hit
AAGCGCCCGGCC814460.10163456751294464No Hit
GTGGGGGGGCCGGGCC806710.10066746305327157No Hit
CCCGCGGGGGCGCGCCGGC805080.10046405914755968No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC804680.10041414407867333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)