FastQCFastQC Report
Mon 3 Jul 2023
SRR10103860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10103860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71056095
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length12-36
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCT38156355.369891210599175No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC24813363.4920804471453155No Hit
TTTTGGTGACTCTAGAT23986763.375749821320747No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC21348773.0044952512518455No Hit
TTTGGTGACTCT16561602.3307782393614507No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC14901162.0970980744157135No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT9625911.3546916700108556No Hit
TTTGGTGACTCTAGAT8465181.1913376326126563No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG7396371.040919853532621No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6889420.9695748126884822No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG6704620.9435671915266381No Hit
AGCTAGGAATAATGGAAT5223930.7351839416449778No Hit
AGGAATAATGGAAT5059790.7120838824593443No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC5025450.7072510809945297No Hit
TTTTGGTGACTCTAGATAACCTC4850720.682660650011797No Hit
CCGCCGAGGGCGCACC3888530.5472479172968905No Hit
TCGCTTCTGGCGCCAAGCGCCCGG3785800.5327903257278633No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT3608620.5078550967373031No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3401280.4786753339034463No Hit
CTTTGGTGACTCT3305270.46516347401303154No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCT3042670.4282067569291558No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC2911740.40978046992309386No Hit
TTTGGTGACTCTAGATAACCTC2897800.40781863962549586No Hit
AGATAACCTCGGGCCGATCGCAC2692730.3789583427009323No Hit
ACCGCAGCTAGGAAT2612610.36768274417557No Hit
AGGAATAATGGAATA2591960.364776589538167No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2589810.3644740116945633No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2447530.34445039514203535No Hit
AACCTCGGGCCGATCGC2312490.3254456918861077No Hit
CCGGCCGAGGTGGGATCCCGAGGCC2150650.3026693206261898No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT1988640.279869024606545No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1937880.2727253728198826No Hit
CCGCCGAGGGCGC1883150.265023007526659No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1853690.2608769873998845No Hit
TAGGAATAATGGAAT1835310.2582902986717748No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1826450.257043396488366No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1796480.25282560208241106No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC1765380.24844877839121332No Hit
TGGGGGGGCCGGGCCACCCCTCCC1725960.24290104881220959No Hit
CCGCCGAGGGCGCACCACCGGCCC1659440.2335394310649917No Hit
AGATAACCTCGGGCCGATCGC1609440.22650273702769622No Hit
CGGCCGAGGTGGGATCCCGAGGCC1605710.225977799652514No Hit
TTGGTGACTCTAGAT1597030.22475622956763947No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1573780.2214841668402971No Hit
CCCCCGCGGGGGCGCGCCGGC1546330.2176210218138219No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT1538490.21651766818877394No Hit
AACCTCGGGCCGATCGCAC1534330.215932215244871No Hit
CTTTGGTGACTCTAGAT1525990.2147584946794501No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1501900.21136821549228116No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC1493520.21018886557163044No Hit
TCGCTTCTGGCGCC1443790.20319016968213638No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT1404110.19760584929413866No Hit
AAGTCCTTCTGATC1401640.1972582366086963No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1399640.19697676884720444No Hit
AGCTAGGAATAATGGAATA1342830.18898167708202934No Hit
AAATTTTTGACAGTCTCTACGGAGACTG1314660.18501720366141708No Hit
AGAATCCCGCCCAGGCGGAACGAT1310170.18438530853686794No Hit
ACCGGCCCGTCTCG1286930.18111465314833303No Hit
ACCGGCCCGTCTCGC1266710.17826901407965073No Hit
ACGGCGCGACCGCTC1256600.1768461945453096No Hit
TTTTGGTGACTCTAGATAACCT1241130.17466904141017037No Hit
CCGCCGAGGGCGCACCACCGGCCCGT1234310.1737092363434833No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT1221180.1718614004892895No Hit
TTGGTGACTCTAGATAACCTC1160840.1633695181250813No Hit
AGACGACCTGCTT1157330.16287554220366318No Hit
CGGCCGAGGTGGGATCCCGAGGCCT1120370.15767401797129435No Hit
ACCCCTCCCACGGCGCGACCGCTC1117370.15725181632905663No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG1083490.15248375244938525No Hit
GAGGGCGCACCACCGGCCCGTCTCG1080190.15201933064292375No Hit
CGAGGGCGCACCACCGGCCCGTCTCG1063220.14963107668666567No Hit
ACCCCTCCCACGGC1054480.1484010625689464No Hit
TAACCTCGGGCC1041720.1466052982506286No Hit
ACCCCTCCCACGGCGCGACCGCT1037960.14607613885902399No Hit
AGATAACCTCGGGCC997550.14038908273808182No Hit
ACCCCTCCCACGGCGCGACCGC984230.13851450744654628No Hit
ACTGACCCGGTGAGGCGGGG978480.1377052876322573No Hit
TCGGCCGAGGTGGGATCCCGAGGCC977960.13763210601426942No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC971190.13667933764161963No Hit
AGATAACCTCGGGC964630.13575612338392645No Hit
AAATTCTAGAGCT945870.1331159557811332No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG938190.13203511957700462No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG922940.1298889278956295No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC912660.12844218360156154No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG903670.12717698601365585No Hit
AACCTCGGGCCGATC847120.11921848505747468No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGCT835970.11764930228715777No Hit
AATTTTTGACAGTCTCTACGGAGACTG833410.11728902355244825No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC828650.11661913028009772No Hit
AAGCGCCCGGCC827220.11641788083063107No Hit
TTGGGGGGGCCGGGCCACCCCTCCC817290.11502039339482419No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT809650.11394518654592545No Hit
TGCCGCCTGGAT791050.11132753636405152No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG780240.10980620311318826No Hit
TTTTGGTGACTCTAGATAAC773910.10891535764806665No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGT762820.10735461891059452No Hit
AAATTCTAGAGCTAATACATGC756980.10653273304703839No Hit
TCGCTTCTGGCGCCAAGCGCCCG750130.10556870596392892No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC736210.10360969034394586No Hit
ACCCCTCCCACGGCGC735640.1035294720319207No Hit
ACGGCGCGACCGCT733710.10325785564208108No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC731010.10287787416406713No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC725720.10213339193492128No Hit
GTGGGGGGGCCGGGCC716260.10080204942306498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)