FastQCFastQC Report
Tue 27 Jun 2023
SRR1013478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1013478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7187292
Total Bases725.9 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4259895.9269749997634715No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATANNNNNN3224624.486557663164374No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1056791.4703590726521198No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA838971.1672963892381163No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA514330.7156102743564614No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATANNN498540.6936409429309398No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATANNNNNNNNNNNN495070.6888129771268512No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATANNNNNNNNNNNN389290.5416365440558141No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATANNNN286360.3984254431293455No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATANNNNNNNNNNNN252520.35134234145489013No Hit
TGGAGTCGACCCAACGCGACTGTAGGCACCATCAATANNNNNNNNNNNNN220460.30673583319002484No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT202900.28230382180103436No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATANNNN188510.2622823728324938No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATANNNNN175670.24441750801275364No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT174480.24276180792431973No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATANNNN173830.24185743392643572No Hit
AGCTCAACCCGCAACGCGACTGTAGGCACCATCAATANNNNNNNNNNNNN173720.2417043860191015No Hit
ATGTACACGGAGTCGAGCTCCTGTAGGCACCATCAATANNNNNNNNNNNN160290.22301862787820503No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATANNN151420.21067740116861816No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAN150890.20993998852418963No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATANN142080.1976822424913305No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATANNNNNNNNNNNNNNN140910.19605436929513922No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATANN130550.18164003911347976No Hit
ATGTACACGGAGTCGACCCCTGTAGGCACCATCAATANNNNNNNNNNNNN120530.1676987660999442No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATANNNNN120390.1675039778542461No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATANNNNN118930.16547261472053731No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAN115780.16108987919233No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATANNNNNNNNN114910.15987940937977752No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATANNNNNNN112140.15602538480417938No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA110180.15329834936440595No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATANNNNNN107710.14986172817244658No Hit
ACGTTGAACGCACGTTCGCTGTAGGCACCATCAATANNNNNNNNNNNNNN107450.14949997857329297No Hit
ATGTACACGGAGTCGAGCTCAACCCGCCTGTAGGCACCATCAATANNNNN101890.14176410252985408No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATANNNNNNNNNNNNN99540.13849444269135022No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA92880.1292280875745691No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATANNNN92590.12882459763705162No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT91150.12682106139558544No Hit
ACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATANNNNNNNNNNNN89330.1242888142015101No Hit
TCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATANNNNNNNNNN88640.1233287864191409No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA87020.12107480814749143No Hit
ACGACGCTCTTCCGATCTCTGTAGGCACCATCAATANNNNNNNNNNNNNN85280.11865386852238646No Hit
TGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATANNNNNNNNNNN84890.11811124412365603No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAN84340.11734600458698492No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATANNNNNNNN83830.11663641883479899No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAN83010.11549551625285295No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATANNNNNN82030.11413199853296625No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATANNNNNNNNNNNNN80920.11258760601350272No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATANNN79100.1100553588194274No Hit
ATCTCAACCAGCCACTGCTAACTGTAGGCACCATCAATANNNNNNNNNNN78650.10942925374396921No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATANNN78140.10871966799178327No Hit
ACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATANNNNNNNNNNNNN77410.10770398642492889No Hit
CAGTGCAGTGCTTGATAACAGGCTGTAGGCACCATCAATANNNNNNNNNN74130.1031403760971448No Hit
ACGGAGTCGAGCTCAACCCGCCTGTAGGCACCATCAATANNNNNNNNNNN72520.10090031127161664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)