FastQCFastQC Report
Thu 29 Jun 2023
SRR1013479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1013479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66444289
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGGCGCTCTCGCTCCCGACGTCTGTAGGCACCATCAATATCAGTATC11783191.773393948123969No Hit
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATAT10947281.6475878009620963No Hit
TAAGGGAAGTCGGCAAGCCGGATCCGTCTGTAGGCACCATCAATATCAGT6301330.9483629210028871No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATATCAGTA5960640.8970883863321948No Hit
AAATCGGTCGTCCGACCTGGGTCTGTAGGCACCATCAATATCAGTATCTC5918360.8907251607433109No Hit
AGGAGGGCCGCTGCGGTGAGCCTTCTGTAGGCACCATCAATATCAGTATC5694780.8570759181424907No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCACCATCAATATC5330380.8022329804748155No Hit
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATATCAGTAT4544980.6840286905621038No Hit
CTGTAGGCACCATCAATATCAGTATCTCGTATGCCGTCTTCTGCTTGAAA4438510.6680047400311561RNA PCR Primer, Index 40 (96% over 29bp)
AACTGGCTTGTGGCGGCCAAGCGTTCCTGTAGGCACCATCAATATCAGTA3902050.5872664240564001No Hit
TAGCGGTGGATCACTCGGCTCGTCTGTAGGCACCATCAATATCAGTATCT3803660.5724585298820791No Hit
CAGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATAT3689510.5552787238042385No Hit
CGCGACCTCAGATCAGACGTCTGTAGGCACCATCAATATCAGTATCTCGT3032570.4564079239376013No Hit
TAGTCGGTCCTGAGAGATGGGCGAGTCTGTAGGCACCATCAATATCAGTA2791530.4201309159918921No Hit
TCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATATCAGTA2445260.3680165800254105No Hit
GGAGCAGAAGGGCAAAAGCTCGCTTCTGTAGGCACCATCAATATCAGTAT2409540.3626406477161641No Hit
AGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATATCAGTATC2349220.35356236560827675No Hit
AGCGGTGGATCACTCGGCTCGTCTGTAGGCACCATCAATATCAGTATCTC2307190.34723676552547655No Hit
ACGGAAGACCCACTCCCCGGCGCCTGTAGGCACCATCAATATCAGTATCT2245000.3378770446320827No Hit
TGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAATATCAGT2187510.3292246832530633No Hit
TATCCGCAGCAGGTCTCCAAGGTCTGTAGGCACCATCAATATCAGTATCT2127930.32025777264318384No Hit
ACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATATCAGTATCT2081490.31326845863306624No Hit
GGCGCGACCCGCTCCGGGGACAGTGCCAGGTCTGTAGGCACCATCAATAT2061770.31030055871318No Hit
AACTGGCTTGTGGCGGCCAAGCGTTCATCTGTAGGCACCATCAATATCAG2060600.31012447134470805No Hit
ACGAGAGGAACCGCAGGTTCAGACCTGTAGGCACCATCAATATCAGTATC2057250.30962028956318577No Hit
ATGTGCTTGGCTGAGGAGCCAATCTGTAGGCACCATCAATATCAGTATCT2050480.3086013908584378No Hit
CAAGGGAAGTCGGCAAGCCGGATCCGTCTGTAGGCACCATCAATATCAGT1976790.29751089668519143No Hit
TGAAGCCTAGGGCGCGGGCCCGGGTCTGTAGGCACCATCAATATCAGTAT1958510.29475971968034753No Hit
ACGCGTTAGGACCCGAAAGATCTGTAGGCACCATCAATATCAGTATCTCG1930560.2905531881001842No Hit
TGGCGGGAGTAACTATGACTCTCTTCTGTAGGCACCATCAATATCAGTAT1882940.28338628170135133No Hit
AAGAGGGACGGCCGGGGGCATTCGTCTGTAGGCACCATCAATATCAGTAT1879960.28293778566883304No Hit
TGGCCATGGAAGTCGGAATCCGCTCTGTAGGCACCATCAATATCAGTATC1873510.28196704761187225No Hit
TAGCTGGCGCTCTCGCTCCCGACGTCTGTAGGCACCATCAATATCAGTAT1863770.28050115789484936No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAATA1818190.2736412756256599No Hit
TGAGCTTGACTCTAGTCTGGCACGGTCTGTAGGCACCATCAATATCAGTA1731210.2605506095490013No Hit
AACACGGACCAAGGAGTCTCTGTAGGCACCATCAATATCAGTATCTCGTA1670580.25142567181357No Hit
TCGGGTTGCTTGGGAATGCAGCCCAAAGCCTGTAGGCACCATCAATATCA1629080.2451798378036674No Hit
GGGTTCGCCCCGAGAGAGGGGCCCGTCTGTAGGCACCATCAATATCAGTA1610900.242443711001257No Hit
TGAACGCGCCCGATCTCGTCTCTGTAGGCACCATCAATATCAGTATCTCG1599950.2407957138347887No Hit
TAATCCTGCTCAGTACGAGAGGAACCGCCTGTAGGCACCATCAATATCAG1581820.23806711213359513No Hit
GCAAATCGGTCGTCCGACCTGGGTCTGTAGGCACCATCAATATCAGTATC1570790.23640707480518003No Hit
TAAGGATTGGCTCTAAGGGCTGCTGTAGGCACCATCAATATCAGTATCTC1529300.23016274581552074No Hit
ACGGAAGACCCACTCCCCGGCGCTGTAGGCACCATCAATATCAGTATCTC1513350.22776223852737743No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCCTGTAGGCACCATCAATATCA1436520.2161991679977191No Hit
ACGATCCTTCTGACCTTTTGGGTTTTCTGTAGGCACCATCAATATCAGTA1423500.21423963164087736No Hit
AAGGGAAGTCGGCAAGCCGGATCCGTCTGTAGGCACCATCAATATCAGTA1414420.21287307325991556No Hit
AGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATATCAGTAT1350290.20322137843931173No Hit
TTAGCGGTGGATCACTCGGCTCGTCTGTAGGCACCATCAATATCAGTATC1324550.19934745633292875No Hit
ATGGTTGCAAAGCTGAAACTTCTGTAGGCACCATCAATATCAGTATCTCG1298620.19544493884192213No Hit
TGCAAATCGGTCGTCCGACCTGGGTCTGTAGGCACCATCAATATCAGTAT1295750.1950129980320807No Hit
GCGGACCAGGGGAATCCGACTGTTTCTGTAGGCACCATCAATATCAGTAT1284150.19326717454979464No Hit
TACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATATCAGTATCTCG1279150.19251466442811963No Hit
CAGCGGTGGATCACTCGGCTCGTCTGTAGGCACCATCAATATCAGTATCT1277210.1922226905009097No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATATC1272180.19146566531850465No Hit
AAGGCGCCCGATGCCGACGCTCATCCTGTAGGCACCATCAATATCAGTAT1244370.18728020402174822No Hit
AGCCAAGGGAACGGGCTTGGCGGAATCCTGTAGGCACCATCAATATCAGT1234090.18573304321158438No Hit
CCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATATCAGTATC1224880.184346919567459No Hit
TAGTCAGCGGAGGAAAAGAAACTCTGTAGGCACCATCAATATCAGTATCT1222770.18402936029611214No Hit
TGAAACTGCGAATGGCTCATTCTGTAGGCACCATCAATATCAGTATCTCG1210220.18214055989070782No Hit
GTGAAACTGCGAATGGCTCATTCTGTAGGCACCATCAATATCAGTATCTC1209020.18195995746150584No Hit
CCGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCACCATCAATATC1208740.18191781689469202No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATATCAGT1200860.18073186094293223No Hit
TGCGGACCAGGGGAATCCGACTGTTTCTGTAGGCACCATCAATATCAGTA1189070.17895744207602252No Hit
AAATACCGGCACGAGACCGATCTGTAGGCACCATCAATATCAGTATCTCG1177360.1771950633710596No Hit
AGGATAGCTGGCGCTCTCGCTCCTGTAGGCACCATCAATATCAGTATCTC1163220.17506696474696268No Hit
GAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAATATCAGTA1162070.1748938874189774No Hit
AAATCGGTCGTCCGACCTGGGTATCTGTAGGCACCATCAATATCAGTATC1155520.17390809915958316No Hit
ACAGCCAAGGGAACGGGCTTGGCGGAATCCTGTAGGCACCATCAATATCA1125730.1694246438546434No Hit
CGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATATCAGTATCTC1116280.16800239972467762No Hit
TGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCAGTA1066280.16047729850792744No Hit
TGGAGCAGAAGGGCAAAAGCTCGCTTCTGTAGGCACCATCAATATCAGTA1055810.15890154231313997No Hit
GAATGGATGAACGAGATTCCCACTCTGTAGGCACCATCAATATCAGTATC1052450.15839585551137436No Hit
GGATGGCGCTGGAGCGTCGGGCCCATCTGTAGGCACCATCAATATCAGTA1042980.1569706013409219No Hit
TACGGACCAAGGAGTCTAACGCGTCTGTAGGCACCATCAATATCAGTATC1040140.15654317559181044No Hit
CGGAGGTTCGAAGACGATCAGATCTGTAGGCACCATCAATATCAGTATCT1038710.15632795769701138No Hit
TCGCTGAGAAGACGGTCGAACTTGACTCTGTAGGCACCATCAATATCAGT1026730.15452494344547807No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATATCAGTA1026720.15452343842523472No Hit
GATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCAGTAT1017670.15316139510500293No Hit
TTACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATATCAGTATCTC1007240.15159165899118884No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTCTGTAGGCACCATCAATATCAG983080.14795553008325515No Hit
TGAACAGCCTCTGGCATGTTGGAACCTGTAGGCACCATCAATATCAGTAT966910.14552191234975814No Hit
TGTCGGCTACCCACCCGACCCGTCTTCTGTAGGCACCATCAATATCAGTA957300.14407558789589878No Hit
AAAGGGAAGTCGGCAAGCCGGATCCGTCTGTAGGCACCATCAATATCAGT954350.14363160692411053No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCAGTA944740.14218528247025114No Hit
GCGAGTGCCTAGTGGGCCACTTTTGGTCTGTAGGCACCATCAATATCAGT941600.1417127061138392No Hit
TTGAGCTTGACTCTAGTCTGGCACGGTCTGTAGGCACCATCAATATCAGT934440.14063511161960057No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATATCAGTATC914020.13756186028267983No Hit
CAGTCGGTCCTGAGAGATGGGCGAGTCTGTAGGCACCATCAATATCAGTA912640.1373541674890975No Hit
TGGATGGCGCTGGAGCGTCGGGCCCATCTGTAGGCACCATCAATATCAGT904330.13610349566687363No Hit
TAGACGACCTGCTTCTGGGTCGGGGTTTCCTGTAGGCACCATCAATATCA900230.13548643736710014No Hit
ACGGACCAAGGAGTCTAACGCGTCTGTAGGCACCATCAATATCAGTATCT894900.13468426157739455No Hit
CATCCGCAGCAGGTCTCCAAGGTCTGTAGGCACCATCAATATCAGTATCT879500.1323665304026355No Hit
GATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATCAGT857930.12912020173772948No Hit
ATGACTGAACGCCTCTAAGTCCTGTAGGCACCATCAATATCAGTATCTCG837080.12598223453034468No Hit
TATGGTTGCAAAGCTGAAACTTCTGTAGGCACCATCAATATCAGTATCTC817100.12297520408413129No Hit
ACCCACTCCCGACCCGGGGAGGTCTGTAGGCACCATCAATATCAGTATCT813170.12238373112849472No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATATCAGTATCT795080.11966114950827451No Hit
TGATCTTGATTTTCAGTACGAATCTGTAGGCACCATCAATATCAGTATCT782860.11782201477090078No Hit
TGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCACCATCAATATCA782640.11778890432554708No Hit
TGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATCAG772520.11626582383927685No Hit
CGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATATCAGTATCT756450.11384725630821334No Hit
AGCTGGCGCTCTCGCTCCCGACGTACGCCTGTAGGCACCATCAATATCAG754720.11358688780611378No Hit
TGGGCCGCCGGTGAAATACCACTCTGTAGGCACCATCAATATCAGTATCT746800.11239491177338055No Hit
TTATCCGCAGCAGGTCTCCAAGGTCTGTAGGCACCATCAATATCAGTATC733070.11032851897926096No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATATCA729370.10977166148922145No Hit
GAGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATAT727610.10950677792639182No Hit
TCGGCGACGACCCATTCGAACGTCTGTAGGCACCATCAATATCAGTATCT717550.10799272756158171No Hit
CGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATATCAGTATCT714400.10751864618492644No Hit
AAGTGGCGTTCAGCCACCCGAGATTCTGTAGGCACCATCAATATCAGTAT704660.1060527564679035No Hit
TAAGCAGGGTCGGGCCTGGTTAGTCTGTAGGCACCATCAATATCAGTATC698130.10506997824899593No Hit
TGCCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATATCAGTATC692070.10415793598152583No Hit
TGAGTGCCTAGTGGGCCACTTTTGGTCTGTAGGCACCATCAATATCAGTA688350.10359806845099961No Hit
TGATCTTGATTTTCAGTACGAATACCTGTAGGCACCATCAATATCAGTAT675760.10170324796462192No Hit
CCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATATCAGTATCT672670.10123819670942674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)