FastQCFastQC Report
Thu 29 Jun 2023
SRR1013480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1013480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69833324
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATCGACGTATCTCGTATGCCGTCTTCTGCTTGAAA12206881.7480021429310741Illumina PCR Primer Index 2 (96% over 30bp)
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATCG12040821.7242226648125758No Hit
TAAGGGAAGTCGGCAAGCCGGATCCGTCTGTAGGCACCATCAATCGACGT9980801.4292316945989854No Hit
AGCTGGCGCTCTCGCTCCCGACGTCTGTAGGCACCATCAATCGACGTATC9881451.415004962387298No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATCGACGTA6487940.9290607446954695No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCACCATCAATCGA6464520.9257070449632328No Hit
TAGCGGTGGATCACTCGGCTCGTCTGTAGGCACCATCAATCGACGTATCT4011510.5744406495672467No Hit
CAGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATCG3821890.5472874239811354No Hit
AGGAGGGCCGCTGCGGTGAGCCTTCTGTAGGCACCATCAATCGACGTATC3615710.5177628376962266No Hit
AACTGGCTTGTGGCGGCCAAGCGTTCCTGTAGGCACCATCAATCGACGTA2849270.40801007839752834No Hit
AAGGGAAGTCGGCAAGCCGGATCCGTCTGTAGGCACCATCAATCGACGTA2744900.39306449167449053No Hit
GGAGCAGAAGGGCAAAAGCTCGCTTCTGTAGGCACCATCAATCGACGTAT2741290.3925475464980014No Hit
AGCGGTGGATCACTCGGCTCGTCTGTAGGCACCATCAATCGACGTATCTC2722060.3897938468459557No Hit
AACACGGACCAAGGAGTCTCTGTAGGCACCATCAATCGACGTATCTCGTA2711830.38832893018238684No Hit
CAAGGGAAGTCGGCAAGCCGGATCCGTCTGTAGGCACCATCAATCGACGT2710500.3881384766963119No Hit
ACGGAAGACCCACTCCCCGGCGCCTGTAGGCACCATCAATCGACGTATCT2707160.3876601950094771No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAATC2626830.37615709084677107No Hit
TGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAATCGACGT2467670.35336567968610516No Hit
ACGAGAGGAACCGCAGGTTCAGACCTGTAGGCACCATCAATCGACGTATC2455640.351643006424841No Hit
TATCCGCAGCAGGTCTCCAAGGTCTGTAGGCACCATCAATCGACGTATCT2387000.3418138881660566No Hit
AAGAGGGACGGCCGGGGGCATTCGTCTGTAGGCACCATCAATCGACGTAT2378920.340656847438624No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTCTGTAGGCACCATCAATCGACG2363940.338511739753359No Hit
TAGTCGGTCCTGAGAGATGGGCGAGTCTGTAGGCACCATCAATCGACGTA2273870.32561388600090124No Hit
TAGTCAGCGGAGGAAAAGAAACTCTGTAGGCACCATCAATCGACGTATCT2135390.3057838117515357No Hit
TGAGCTTGACTCTAGTCTGGCACGGTCTGTAGGCACCATCAATCGACGTA2051680.29379669797760166No Hit
AAATCGGTCGTCCGACCTGGGTCTGTAGGCACCATCAATCGACGTATCTC2024900.2899618525963335No Hit
GGGTTCGCCCCGAGAGAGGGGCCCGTCTGTAGGCACCATCAATCGACGTA1884470.26985254203279796No Hit
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATCGACGTAT1881630.2694458594008786No Hit
AAAGGGAAGTCGGCAAGCCGGATCCGTCTGTAGGCACCATCAATCGACGT1843890.26404156273586515No Hit
AACTGGCTTGTGGCGGCCAAGCGTTCATCTGTAGGCACCATCAATCGACG1789520.256255881504366No Hit
ACGCGTTAGGACCCGAAAGATCTGTAGGCACCATCAATCGACGTATCTCG1697530.24308308738103315No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATCGA1625990.2328386946037396No Hit
AAATCGGTCGTCCGACCTGGGTATCTGTAGGCACCATCAATCGACGTATC1577600.22590933806902846No Hit
CGCGACCTCAGATCAGACGTCTGTAGGCACCATCAATCGACGTATCTCGT1527890.2187909600293407No Hit
TGGCCATGGAAGTCGGAATCCGCTCTGTAGGCACCATCAATCGACGTATC1522920.21807926542348177No Hit
CCGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCACCATCAATCGA1515850.21706685478697824No Hit
GAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAATCGACGTA1515150.21696661611009666No Hit
GCAAATCGGTCGTCCGACCTGGGTCTGTAGGCACCATCAATCGACGTATC1499290.21469549408818062No Hit
ACGGAAGACCCACTCCCCGGCGCTGTAGGCACCATCAATCGACGTATCTC1499160.2146768783339026No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATCGACGT1496710.21432604296481716No Hit
TGAAGCCTAGGGCGCGGGCCCGGGTCTGTAGGCACCATCAATCGACGTAT1458010.20878427611436629No Hit
TTAGCGGTGGATCACTCGGCTCGTCTGTAGGCACCATCAATCGACGTATC1451500.2078520564193679No Hit
AGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATCGACGTAT1431910.2050468054477831No Hit
CAGCGGTGGATCACTCGGCTCGTCTGTAGGCACCATCAATCGACGTATCT1418050.20306207964552853No Hit
GCGGACCAGGGGAATCCGACTGTTTCTGTAGGCACCATCAATCGACGTAT1411970.20219143513775742No Hit
ACAGCCAAGGGAACGGGCTTGGCGGAATCCTGTAGGCACCATCAATCGAC1410510.2019823658974045No Hit
GTGAAACTGCGAATGGCTCATTCTGTAGGCACCATCAATCGACGTATCTC1402380.20081816526448032No Hit
TAGCTGGCGCTCTCGCTCCCGACGTCTGTAGGCACCATCAATCGACGTAT1390840.1991656590770332No Hit
GGCGCGACCCGCTCCGGGGACAGTGCCAGGTCTGTAGGCACCATCAATCG1369530.19611410735653942No Hit
TAAGGATTGGCTCTAAGGGCTGCTGTAGGCACCATCAATCGACGTATCTC1348250.19306685157934053No Hit
GAATGGATGAACGAGATTCCCACTCTGTAGGCACCATCAATCGACGTATC1331650.1906897629561497No Hit
AGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATCGACGTATC1323760.18955992986958492No Hit
AAGGCGCCCGATGCCGACGCTCATCCTGTAGGCACCATCAATCGACGTAT1231080.17628832905046882No Hit
GAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATCGACGTA1175860.16838092942561347No Hit
TGCGGACCAGGGGAATCCGACTGTTTCTGTAGGCACCATCAATCGACGTA1172890.1679556310394161No Hit
AGCCAAGGGAACGGGCTTGGCGGAATCCTGTAGGCACCATCAATCGACGT1155940.16552842307778448No Hit
CGGAGGTTCGAAGACGATCAGATCTGTAGGCACCATCAATCGACGTATCT1140740.1633518118083567No Hit
CCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATCGACGTATC1112790.1593494246385866No Hit
TGCAAATCGGTCGTCCGACCTGGGTCTGTAGGCACCATCAATCGACGTAT1106400.15843438871676793No Hit
GTGGCGTACGGAAGACCCACTCCCCGGCGCCTGTAGGCACCATCAATCGA1095400.15685920950862942No Hit
TAATCCTGCTCAGTACGAGAGGAACCGCCTGTAGGCACCATCAATCGACG1067190.1528195908303033No Hit
TGAACGCGCCCGATCTCGTCTCTGTAGGCACCATCAATCGACGTATCTCG1060110.15180574821270143No Hit
AGGATAGCTGGCGCTCTCGCTCCCGACGTCTGTAGGCACCATCAATCGAC1012350.14496660648718368No Hit
TTGAGCTTGACTCTAGTCTGGCACGGTCTGTAGGCACCATCAATCGACGT1007390.14425634386242306No Hit
TGAAACTGCGAATGGCTCATTCTGTAGGCACCATCAATCGACGTATCTCG996510.14269834842746423No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCCTGTAGGCACCATCAATCGAC992510.14212555598814114No Hit
GAGAAGACGGTCGAACTTGACTCTGTAGGCACCATCAATCGACGTATCTC989880.1417489449592862No Hit
TGGCGGGAGTAACTATGACTCTCTTCTGTAGGCACCATCAATCGACGTAT970080.13891362238463686No Hit
CATCCGCAGCAGGTCTCCAAGGTCTGTAGGCACCATCAATCGACGTATCT961900.13774226184622115No Hit
ATGTGCTTGGCTGAGGAGCCAATCTGTAGGCACCATCAATCGACGTATCT960890.13759763175529208No Hit
TCGCTGAGAAGACGGTCGAACTTGACTCTGTAGGCACCATCAATCGACGT954600.1366969156444565No Hit
AACACGGACCAAGGAGTCTAACGCGTCTGTAGGCACCATCAATCGACGTA949050.13590216613489572No Hit
TGAGAAGACGGTCGAACTTGACTCTGTAGGCACCATCAATCGACGTATCT939860.1345861755055509No Hit
TGAAGAGAGAGTTCAAGAGGGCGTCTGTAGGCACCATCAATCGACGTATC925220.13248975517762837No Hit
ACGATCCTTCTGACCTTTTGGGTTTTCTGTAGGCACCATCAATCGACGTA923420.132231998579933No Hit
TGGGCCGCCGGTGAAATACCACTCTGTAGGCACCATCAATCGACGTATCT910010.1303117119271023No Hit
GGGAGGTGGAGCACGAGCGTCTGTAGGCACCATCAATCGACGTATCTCGT905290.12963581684870107No Hit
ACCCACTCCCGACCCGGGGAGGTCTGTAGGCACCATCAATCGACGTATCT904140.12947113902239568No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATCGACGTA895760.12827113886201377No Hit
ATGGTTGCAAAGCTGAAACTTCTGTAGGCACCATCAATCGACGTATCTCG893240.12791027962524024No Hit
AGGACCCGAAAGATGGTGAACTCTGTAGGCACCATCAATCGACGTATCTC890250.12748211727684622No Hit
GAGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATCG886510.12694655634607913No Hit
AAGTGGCGTTCAGCCACCCGAGATTCTGTAGGCACCATCAATCGACGTAT874090.12516803582198094No Hit
TCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATCGACGTA865510.12393939603963287No Hit
TCCCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATCGACG865150.1238878447200938No Hit
ACACCTGTCAAACGGTAACGCAGGTGTCCTCTGTAGGCACCATCAATCGA859540.12308450332394316No Hit
TGGAGCAGAAGGGCAAAAGCTCGCTTCTGTAGGCACCATCAATCGACGTA857930.12285395436711562No Hit
TGATCTTGATTTTCAGTACGAATACCTGTAGGCACCATCAATCGACGTAT849890.1217026415640762No Hit
CGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATCGACGTATCT829400.11876851229364364No Hit
TTATCCGCAGCAGGTCTCCAAGGTCTGTAGGCACCATCAATCGACGTATC814630.11665347621144312No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATCGACGTA810020.11599333292512326No Hit
TCGGGTTGCTTGGGAATGCAGCCCAAAGCCTGTAGGCACCATCAATCGAC808060.11571266462985495No Hit
TGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCACCATCAATCGAC789910.1131136189364264No Hit
ACCTGGTTGATCCTGCCAGTAGCATCTGTAGGCACCATCAATCGACGTAT786370.11260669762762546No Hit
AACGAACGAGACTCTGGCATGCTCTGTAGGCACCATCAATCGACGTATCT780840.11181481208026127No Hit
TGAACAGCCTCTGGCATGTTGGAACCTGTAGGCACCATCAATCGACGTAT776930.11125490747082296No Hit
AGCTGGCGCTCTCGCTCCCGACGTACGCCTGTAGGCACCATCAATCGACG775290.11102006257070049No Hit
TGAACAGCCTCTGGCATGTTGGAACAATGTCTGTAGGCACCATCAATCGA765470.10961385713216228No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAAT756390.10831361829489886No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTCTGTAGGCACCATCAATCGA752680.10778235330742669No Hit
TATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATCGACGTATC747690.10706779473937114No Hit
CCCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATCGACGT742870.1063775798499868No Hit
TGGCCCTTGAAAATCCGGGGGAGAGCTGTAGGCACCATCAATCGACGTAT742000.10625299749443404No Hit
TAAGCGGTGGATCACTCGGCTCGTCTGTAGGCACCATCAATCGACGTATC730630.10462483498565812No Hit
TATGCTTGTCTCAAAGATTAAGCCATCTGTAGGCACCATCAATCGACGTA725930.10395180386945349No Hit
AGTCAGCGGAGGAAAAGAAACTCTGTAGGCACCATCAATCGACGTATCTC724940.10381003774072105No Hit
TACGGACCAAGGAGTCTAACGCGTCTGTAGGCACCATCAATCGACGTATC722110.10340478708989993No Hit
TGATCTTGATTTTCAGTACGAATCTGTAGGCACCATCAATCGACGTATCT719530.10303533596653655No Hit
CAGTCGGTCCTGAGAGATGGGCGAGTCTGTAGGCACCATCAATCGACGTA715270.10242531201865746No Hit
AAGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATCG712560.10203724514101606No Hit
ACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATCGACGTATCT707560.10132125459186218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)