FastQCFastQC Report
Fri 29 Sept 2023
SRR10149785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10149785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40982750
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA611570914.922641843214524Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT30493407.440545107392745No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT11482302.801739756360908No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT9287652.2662339642898535No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG3821880.9325582104665988No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC3307620.8070761478914908No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT3055370.7455258614905051No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT2432760.5936058463621889No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2423230.5912804777619852No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCGTATGCCGTC2406900.5872958744837767No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC2311530.5640251081247598No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG2130140.5197650230889825No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTCGTATGCCGT2026920.49457881669726894No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA1935920.4723743526239698Illumina Single End Adapter 1 (95% over 22bp)
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA1920650.46864839475145037No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATTCGTATGCCGT1668680.40716642977838235No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1645400.40148599105721305No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1641600.4005587716783281No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1571030.38333933179203444Illumina Single End Adapter 1 (95% over 22bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA1555990.3796694950924474No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG1547890.3776930537848241No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1505690.36739603857720626Illumina Single End Adapter 1 (95% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATTC1322800.32276994589186914No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1301850.3176580390530162Illumina Single End Adapter 1 (95% over 22bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1298690.3168869829379434Illumina Single End Adapter 1 (95% over 22bp)
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1298360.31680646125504025No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATTCGTATGCC1295420.31608908626190285No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATTCGTATGCCGT1193740.2912786477237374No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATTCG1166850.28471735059262737No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1157680.282479823828318No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATTCG1033170.25209874886385125No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTATG1029400.25117884963795745No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1014290.24749193258139096No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC1004970.24521780505212556No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG984590.2402449811201054No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATTCGTA976520.2382758599654733No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG958760.23394232939468437No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGTATGCCG942490.22997236642245825No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT922820.2251727861112297No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT853270.20820223142663682No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG846430.20653323654464378No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT830120.2025535133684294No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT799210.19501131573649888No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATTC768570.18753499948148916No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT767270.1872177928518706No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT762510.18605632857726725No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG746490.1821473668799678No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTAT712140.17376579170504663No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT699780.17074988867267327No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA694120.169368819808334No Hit
CGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA674290.16453019868115243Illumina Single End Adapter 1 (95% over 22bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATTCGTA673880.16443015659027274No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG673060.16423007240851334Illumina Single End Adapter 1 (95% over 22bp)
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG661260.16135081223197567No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTGTAGGCACCATCAATTC627400.15308879955591073No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC624100.15228358272687897Illumina Single End Adapter 1 (95% over 22bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT609630.14875282893412473No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA609240.14865766694523916Illumina Single End Adapter 1 (95% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATTC593850.14490242846075485No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT583460.1423672154748034No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA576960.14078118232671064Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT573360.13990276396776694No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATTCGT567770.1385387754604071No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT554730.13535694896023326No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC544020.13274365434237576No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATTCGTATGCCG533320.1301327997755153No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATTCG527150.1286272883103257No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATTCGTA504720.123154253923907No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATTC494370.12062880114194388No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATT488820.11927457283857233No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG485380.11843519529558169No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATTCGT485330.11842299504059635No Hit
TCACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT475900.11612202695036326No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG474680.11582434072872125No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATTCG460600.11238874892485252No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATTCGT454060.11079295557277147No Hit
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATTCGTAT433670.10581769158975424No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATTC410770.10022997480647346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)