FastQCFastQC Report
Fri 29 Sept 2023
SRR10149787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10149787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22046312
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA256964811.655681911786425Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT20319559.216756979580076No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT9422534.273971084143235No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT6578302.9838550774388026No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1668540.7568340682105924No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1501120.6808939291070543No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG1389280.6301643558342094No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATTC1270780.5764138691314902No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1161330.5267683774048013No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT1016260.46096598832494073No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT954320.4328705862458991No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT934080.4236899123989536No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATTCG866430.3930045079648696No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA792720.35957034446396297Illumina Single End Adapter 1 (95% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTGTAGGCACCATCAATTC766690.3477633810135682No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC764510.34677455349448016No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC761710.3455044998002387No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT733690.3327948910457223No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATTCGTATGCC654430.296843299686587No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT649130.29443926947962995Illumina Single End Adapter 1 (95% over 22bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA619010.2807771204544325Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC592170.2686027486139178No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA578290.26230691101532083No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA571920.2594175388609215Illumina Single End Adapter 1 (95% over 22bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG561040.25448247307758326No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT512600.23251054416720585No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA503360.22831936697620897Illumina Single End Adapter 1 (95% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT494760.22441848777246734No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG477350.21652147533791594No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATTCGTATGCCGT443050.20096331758345792No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT436470.1979786914019905No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATTC434390.19703522294341114No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC434270.19698079207080077No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG427250.19379658602309538No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCA402740.18267908029243166No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATTCGTA368490.167143602068228No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCA363250.1647667872975761No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATTC358920.1628027399775527No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA358780.16273923729284062No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATTCG352080.15970018023876284No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCTGTAGGCACCATCAATTC348500.1580763258725541No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATTCGTATGCCGT345440.15668833862099021No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC335800.15231572518795888No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT330010.14968943558450956No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACTGTAG317090.14382904496679536No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC300990.13652623622490692No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATTCG298330.13531968521537752No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCAT296450.13446693487781539No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATTC291830.13237134628231698No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG288320.13077924325846427No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTCGTATGCCGT288140.13069759694954874No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG282890.128316246272846No Hit
CGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA281690.12777193754674251Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG280450.12720948519643557Illumina Single End Adapter 1 (95% over 22bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATT279850.12693733083338382No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT271580.12318613652932064No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC260660.11823292712177891Illumina Single End Adapter 1 (95% over 22bp)
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGCTGTAGGCACCATCAAT258010.11703091201830038No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT256470.11633238248646759No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA250860.11378773919193377Illumina Single End Adapter 1 (95% over 22bp)
GTCCCGCGCGTGTGGGGGGAACTCCGCGTCGGTCTGTAGGCACCATCAAT250160.11347022576837341No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA249620.11322528684162685No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATTC247090.11207770261075867No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT246440.1117828687174526No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATT238990.10840361870956011No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGACTGTAGGCACCATCAA238030.10796817172867734No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATTCGT234490.10636246098667204No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA231560.10503344051376938Illumina Single End Adapter 1 (95% over 22bp)
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATTCGTATGCCG230120.10438027004244518No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCGTATGCCGTC229050.10389492809500292No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC227740.10330072440233995No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCTGTAGGCACCATCAATTCG226700.10282899017305025No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTCTGTAGGCACCATCAA226490.10273373614598214No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATTC222250.10081051198041649No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)