FastQCFastQC Report
Fri 29 Sept 2023
SRR10149791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10149791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25251640
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA399885915.836036788105645Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT20925468.286772661102408No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7971793.156939509671451No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT6816562.699452392003054No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT2170630.8595996141240727No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1997420.7910060495080716No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1811810.7175019127470533No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1601820.6343429575267191No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG1235960.48945731841575435No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG1227660.4861704031896542No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA1215740.4814499177083152Illumina Single End Adapter 1 (95% over 22bp)
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1185640.4695298998401688No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG1078210.4269861284257181No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG1008540.3993958412206098No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG998380.3953723401727571No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT996550.3946476347674844Illumina Single End Adapter 1 (95% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC929240.36799194032546007No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA926510.36691082242579093Illumina Single End Adapter 1 (95% over 22bp)
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT859520.34038185242621866No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC839170.3323229699140333No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA835080.330703273133943No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA830010.32869548274884325Illumina Single End Adapter 1 (95% over 22bp)
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA809680.32064452051431114Illumina Single End Adapter 1 (95% over 22bp)
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTCGTATGCCGT779240.3085898579260594No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTATG760920.30133488359567934No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC757200.29986171195217415No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCGTATGCCGTC736690.2917394672187628No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGTATGCCG711540.2817797180697966No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA700980.27759781146887885No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATTCGTATGCCGT694770.27513856525754365No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATTC627090.24833634567893415No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATTCGTATGCC584790.23158495844230317No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC537680.21292874443006476No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATTCG515890.20429960192684515No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATTCGTATGCCGT503270.19930190672764223No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATTCG498600.19745252189560758No Hit
CCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATTCGTATGCCG482580.1911083794953516No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGTATGCC481220.19056980061493037No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATTCGTA470440.18630077095982675No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATTCGTA464700.18402765127334303No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT464430.1839207275250241No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT462100.1829980151784201No Hit
CGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA452920.17936260773557677Illumina Single End Adapter 1 (95% over 22bp)
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG441530.17485200961204894No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA432230.1711690805032861No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG428570.16971966969274074No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG426410.1688642797061894Illumina Single End Adapter 1 (95% over 22bp)
TCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGTATGC425200.16838510290816755No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT414900.16430615991674205No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATGC410290.16248053591766712No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC400950.1587817662535978Illumina Single End Adapter 1 (95% over 22bp)
TCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATTCGTATGCCG396180.15689278003329685No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT388040.15366922702842273No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT384420.15223565677318385No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA378300.14981205181128832Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT369730.1464182128368692No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATTCG355340.14071957306535338No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA355240.14067997167708712Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT348740.13810588143977975No Hit
TGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGTA345800.1369416006247515No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATTCG337770.13376160914697025No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT324820.12863322936648866No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTAT323580.12814217215198695No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT321420.1272867821654356No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT320490.1269184892545593No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT308030.12198415627658243No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATTCGTA298720.11829726702899297No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT297550.11783393078627766No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC291710.11552120971152764No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTGTAGGCACCATCAATTC280670.11114921644693176No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA280320.11101061158799984No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATTCGT276760.10960080216572071No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG274680.10877709328978237No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATTCG274510.10870977092972971No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATTC274080.10853948496018476No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT269020.10653565471391165No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATTCGTAT265630.1051931676516852No Hit
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATTCGTAT264940.10491991807264796No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC256080.10141123507225669No Hit
TCCCCTCTTCCTCCCCGCGTCCGGCGCTCTGTAGGCACCATCAATTCGTA253290.10030635633962784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)