FastQCFastQC Report
Fri 29 Sept 2023
SRR10149793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10149793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28395947
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT27422429.657159875668173No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA23288648.201395783701104Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT13094734.611478532482118No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT8118652.8590876014805913No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG4857231.7105363663342519No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC2797190.9850666364463915No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC2793550.9837847633678145No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2481430.8738676685091713No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2379940.8381266523704949No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1302030.45852670453286876No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT1293340.4554664086392329No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1274280.4487541831233873No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATTC1245670.43867880159094536No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1225110.4314383316745872No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1218890.4292478782271287No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC1145840.40352237592216944No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1108860.3904993906348677No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1099010.3870305857381689No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA967030.34055212175174154No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT948210.3339244153399779Illumina Single End Adapter 1 (95% over 22bp)
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTCGTATGCCGT924890.32571197572667676No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATTCGTATGCC891710.3140272095873401No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA880210.3099773358500775Illumina Single End Adapter 1 (95% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG877730.30910397177456345No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA862420.3037123572599991No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATT714380.25157815655875115No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT685890.2415450345783502No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT679630.2393404946135447No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC662200.2332022946795893No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATTCGTA645510.22732469531655344No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT626930.22078150800887184No Hit
CCTCCCGACCCCTCCACCCGCGCGTCGCTGTAGGCACCATCAATTCGTAT625830.22039412878182932No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA602800.21228381641929395Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT565480.19914109573454267No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT557310.19626392456641786No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT542680.19111178084675254No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA539120.1898580808028695No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATTCGT527720.18584342335897444No Hit
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG512370.18043772232706307No Hit
GTCCCGCGCGTGTGGGGGGAACTCCGCGTCGGTCTGTAGGCACCATCAAT512210.18038137625767509No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGCTGTAGGCACCATCAATTCGTA496540.17486298308698772No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT495490.17449321200662898No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT486800.1714329161129932No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC484770.1707180253576329No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG480650.16926711407089187No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA476270.16772464042139534Illumina Single End Adapter 1 (95% over 22bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC454140.15993127469916746No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT448580.15797324878793442No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC445560.1569097167282359No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT437700.15414171606955035No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATTCG435370.15332117643408757No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG428640.1509511198904548No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATTC425150.14972207125192902No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT424280.14941568949963177No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG416480.14666881861696673No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT414470.14596097112027995No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG407480.14349935221389165No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCAATTCGT403700.14216817632460013No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGTATGCCG401240.14130185550775962No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATTCGTATGCCGT398410.14030523440545936No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT382050.13454384881053624No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATTC373400.13149763943424742No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT370430.13045171552123266No Hit
TGTCCCGCGCGTGTGGGGGGAACTCCGCGTCGGTCTGTAGGCACCATCAA369910.13026859079572167No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATT369180.1300115118541389No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTATG361400.12727168423014734No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA361150.12718364349672862Illumina Single End Adapter 1 (95% over 22bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATCAATTCGT360730.1270357350645851No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG360670.1270146052885646No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG355990.12536648275896556Illumina Single End Adapter 1 (95% over 22bp)
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCGTATGCCGTC347270.12229562197731952No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCA344560.12134126042706024No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG330010.1162172897420889No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATTC317770.11190681543390682No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTAT316250.1113715277747208No Hit
GACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCG308090.10849787823593277No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGCCTGTCTGTAGGCACCATCAATTCG303260.10679693126628247No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCTGTAGGCACCATCAATTC288320.10153561703717788No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC286930.10104611055936961No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)