FastQCFastQC Report
Fri 29 Sept 2023
SRR10149795.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10149795.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26995651
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT399800014.809792881082956No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA290843210.77370573504599Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT19394527.184312762081567No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT12948104.7963651626700905No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC3958791.466454726355738No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3645791.350510124760466No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG2113280.7828223886877187No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2102750.7789217603976285No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1781460.6599062937952488No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1684640.624041257608494No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC1186900.43966341096941874No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1154440.42763925196691865Illumina Single End Adapter 1 (95% over 22bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1144290.4238793870909059No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC1062520.3935893229616874No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA976520.3617323397757661Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA960610.3558387978863707No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT923190.34197730590012443No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT895770.33182011428433417No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA816750.3025487327569911No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT771430.28576084347808467No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT749610.2776780600697497No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATTC735840.2725772384596319No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA733790.2718178568836884Illumina Single End Adapter 1 (95% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT690390.2557411932759095No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC681000.25226285522805136No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT660350.2446134749630598No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT628100.2326671062683393No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT625510.2317076924723912No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT617010.22855903715750364No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT596380.22091706549325296No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA594240.2201243452139754Illumina Single End Adapter 1 (95% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG557010.20633323493476782No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC553140.2048996706914014No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT542880.20109905851131352No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCA538850.1996062254620198No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT486310.18014383131564413No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATTCGTA484650.17952891745414845No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA483170.17898068099932096No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATTCG461150.17082381158357693No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA456280.16901981730316487Illumina Single End Adapter 1 (95% over 22bp)
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT430390.15942938364405437No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATT426260.15789950759105606No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA425230.15751796465289908No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG422610.15654743795583964Illumina Single End Adapter 1 (95% over 22bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA420560.15578805637989615No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT418470.15501385760247086No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT410410.15202819150388333No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT383430.14203398910439313No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCAT369580.13690353309131165No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATTCGTATGCC369490.13687019438797754No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT369100.13672572667352975No Hit
GTCCCGCGCGTGTGGGGGGAACTCCGCGTCGGTCTGTAGGCACCATCAAT355640.13173973837489603No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT337420.12499050309992524No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA328450.12166774566762624No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT323400.11979707398054598No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC309460.11463327926413036Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT291490.10797665149842098Illumina Single End Adapter 1 (95% over 22bp)
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTCGTATGCCGT282980.10482429188316295No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG277550.10281285678200537No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC272670.10100515820122287No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATTCGT271850.10070140557084546No Hit
TGTCCCGCGCGTGTGGGGGGAACTCCGCGTCGGTCTGTAGGCACCATCAA271460.10055693785639769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)