FastQCFastQC Report
Fri 29 Sept 2023
SRR10149797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10149797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22876477
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA375726716.424150449389565Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT21347009.331419343983779No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT9688934.2353243464891905No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT5327042.3286103013151895No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC3389861.481810332945934No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG3263371.426517728232367No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG1819830.7955027340966879No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1787420.7813353428502124No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1489120.6509393907112533Illumina Single End Adapter 1 (95% over 22bp)
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC1280590.559784620682634No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC1105270.4831469460966389No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT1102100.48176124321939956No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1060550.46359848153192473No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATTC971940.42486437050600057No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA931780.4073092198593341Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA869470.3800716342817996Illumina Single End Adapter 1 (95% over 22bp)
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT828660.3622323489757623No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA739650.3233233858517638Illumina Single End Adapter 1 (95% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA725070.3169500268769531No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC718270.3139775412096889No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATTCG694240.30347330141787127No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT684650.29928122236653837No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT661240.289048003326736No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA618900.2705399087455643Illumina Single End Adapter 1 (95% over 22bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC566510.24763865520027406No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT553070.24176362470497534No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG541700.23679345381721145No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG541680.23678471121230776Illumina Single End Adapter 1 (95% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCA524930.22946277960544365No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT519960.22729024228686961No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA516860.22593513852679328No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT508390.22223264535006856No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATTCGTA476810.20842807220709728No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATTCGTATGCC469630.20528947704666237No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTCGTATGCCGT461120.20156949866013021No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA456360.19948875869304525No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT410360.17938076741449305No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT409790.1791316031747371No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC399860.17479089984004093No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT396290.17323034486472721No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC395050.17268830336069665Illumina Single End Adapter 1 (95% over 22bp)
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATT389140.1701048636116479No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATTC378940.16564613511075155No Hit
GTCCCGCGCGTGTGGGGGGAACTCCGCGTCGGTCTGTAGGCACCATCAAT378070.16526583179743978No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT373530.16328126048429573Illumina Single End Adapter 1 (95% over 22bp)
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT350580.15324912135727892No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA345100.15085364761366007Illumina Single End Adapter 1 (95% over 22bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATTCGT339520.14841446084552268No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATT337230.1474134325840469No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATTC320930.14028820958751645No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATTCGT312230.13648517645439898No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG306090.13380119674895746No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT305270.133442749947905No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC296610.12965720202459496No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT295020.12896216493474935No Hit
CGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA290130.12682459803579021Illumina Single End Adapter 1 (95% over 22bp)
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT288630.12616890266801134No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT280780.12273743024330187No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCTGTAGGCACCATCAATTC279540.12219538873927135No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT277770.12142166820529228No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG273300.11946769600931122No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCAT273140.11939775517008147No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATTCG271300.1185934355189394No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATTCGTA270880.1184098408159613No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATTC265000.11583951497426812No Hit
TGTCCCGCGCGTGTGGGGGGAACTCCGCGTCGGTCTGTAGGCACCATCAA261120.11414344962294674No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGTATGCCG252750.11048466947074062No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTATG250230.10938310125287211No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCAT246020.1075427829206394No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT240170.10498557098630179No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTAT238020.10404574095915205No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT234890.10267752329172013No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATTC230740.1008634327741986No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG230170.1006142685344426No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)