FastQCFastQC Report
Fri 29 Sept 2023
SRR10149799.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10149799.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32336624
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA908518128.095638555218382Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT20653406.38699945918906No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT10356873.2028297078878736No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT7040752.177329952564003No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC3741651.1570935790947132No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT3576451.106005994936268Illumina Single End Adapter 1 (95% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG3550941.0981171070919462No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2792070.8634389291844442No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2443790.7557344266983467Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA2262520.6996772452189196Illumina Single End Adapter 1 (95% over 22bp)
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1895260.5861032369983954No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1742350.5388162969640863Illumina Single End Adapter 1 (95% over 22bp)
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC1517630.4693223386584821No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1422480.4398974982669805Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1305250.40364448682088766Illumina Single End Adapter 1 (95% over 22bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1129470.3492850706987842No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1071780.33144461833739974No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1031220.318901564987118No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1000310.3093427440044452No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC960470.29702234840594366Illumina Single End Adapter 1 (95% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC918880.28416077077186536No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC897300.2774872231560103No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT895100.276806880025571Illumina Single End Adapter 1 (95% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATTC856050.2647307894602727No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA838090.25917671554086785Illumina Single End Adapter 1 (95% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT821210.25395662824913323No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT809520.25034153225148054No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA768550.23767168768143512No Hit
CGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA764400.23638831313992453Illumina Single End Adapter 1 (95% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT762920.23593062776126536No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTA698360.21596564935164536No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG681100.21062804824647124No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATTCG680680.21049816455793285No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT669750.20711809618715915No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA638770.19753762792306334No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT587010.1815310095450904No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATTCGTA535300.16553985351099113No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGT515370.15937656324296562No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTATG505790.15641397815677976No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT505420.15629955681211494No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA476980.14750457561679908Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT473890.14654900276540928No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT473000.14627377304445882No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCA464880.1437626883993827No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG451320.13956930074085658No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTCGTATGCCGT427170.1321009886498974No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT427120.13208552630602377No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATTCGTAT424830.1313773509566119No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT424570.1312969467684691No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATTCGT418260.12934559897161804No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATTCGTATGCC415600.1285230022775414No Hit
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA414980.1283312692135085Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC412570.12758598423879994No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT400670.12390594639687803No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACCATCAATTCGT396130.12250196557315321No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC381310.11791892684901181No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATTC381130.11786326241106679No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATC369710.11433166307033166Illumina Single End Adapter 1 (95% over 22bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC362880.11221950689719495No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT356680.1103021762568659No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG353680.10937443562444862No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATT349640.10812507823946No Hit
TGTAGGCACCATCAATTCGTCTGCCGTCTTCTGCTTGAAAAAAAAAAAAA348720.10784057111218537Illumina DpnII expression Adapter 2 (95% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATTCGT341070.10547483249952129No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCGTATGCCG336570.10408322155089536No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG326510.10097219796352272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)