FastQCFastQC Report
Tue 18 Jul 2023
SRR10174361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43426278
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC37164698.558110828655405RNA PCR Primer, Index 1 (100% over 50bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA21487974.948149136796849RNA PCR Primer, Index 1 (100% over 22bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA14428233.322465259398929No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG12172732.803079278403735Illumina Small RNA Adapter 2 (100% over 21bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG10939342.5190600032542507No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA9933582.2874582988668752RNA PCR Primer, Index 1 (100% over 23bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA8154801.8778491677320355No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG7770701.789400417876015Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA7315031.6844708634711916No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA5816361.3393641518160961No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC5315531.2240353640254409No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG4930971.1354806875228864No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA4654181.0717427821007364No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC4545751.0467740293100873No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC4467221.0286905085441584No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC4441561.022781643870101No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3889480.8956512460036293No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA3807260.8767180093122418RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA3437760.7916312791070881No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA3434900.7909726916960279RNA PCR Primer, Index 1 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC3417140.7868830020385353No Hit
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA3222010.7419493791293833RNA PCR Primer, Index 1 (100% over 23bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC3173160.7307004298180931No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC2375950.547122643114844RNA PCR Primer, Index 1 (100% over 26bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG2345820.5401844477668567No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA2335100.5377158963519738No Hit
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA2331120.5367994005841348RNA PCR Primer, Index 1 (100% over 28bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC2325580.5355236753193539No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT2092530.48185801233069064RNA PCR Primer, Index 1 (100% over 25bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA2078880.47871475423244886RNA PCR Primer, Index 1 (100% over 23bp)
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA2077650.47843151559062924RNA PCR Primer, Index 1 (100% over 23bp)
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2006450.4620359129096903RNA PCR Primer, Index 1 (100% over 30bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG2002360.4610940868568105No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA1852080.42648831198473885RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1849850.4259747980243667No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG1747570.40242223844281566Illumina Small RNA Adapter 2 (100% over 21bp)
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG1736940.3999744118066024Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC1574980.36267902121383744RNA PCR Primer, Index 1 (100% over 27bp)
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG1391900.32052021589324325RNA PCR Primer, Index 1 (100% over 29bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG1390750.3202553992769079Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1357930.3126977633220144No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA1283600.2955813988940061RNA PCR Primer, Index 1 (100% over 22bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA1262350.29068804837476514No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC1259150.2899511673553971No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1254270.28882742380086085No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA1080700.24885853675970113No Hit
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGC1060200.24413789272937458No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1049600.24169697435271797No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCAAG1017850.23438573298867568No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC984100.22661394098752835RNA PCR Primer, Index 1 (100% over 27bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG959030.22084093875141686Illumina Small RNA Adapter 2 (100% over 21bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG956940.22035966333564205No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA950670.2189158370883178No Hit
GGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTC933460.21495279885602905RNA PCR Primer, Index 1 (100% over 26bp)
CTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG881690.20303144561456543No Hit
TGTTTATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCA853200.19647090178900434No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC824980.18997253229945243RNA PCR Primer, Index 1 (100% over 50bp)
TGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAAC799740.184160383259187RNA PCR Primer, Index 1 (100% over 24bp)
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG797980.18375509869853454No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTGC794630.18298367638138363No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG760770.17518655409519554RNA PCR Primer, Index 1 (100% over 49bp)
TTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAAC758870.17474903098994576RNA PCR Primer, Index 1 (100% over 24bp)
TCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCCAAG751510.17305420464539925No Hit
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC740800.17058795598370186RNA PCR Primer, Index 1 (100% over 31bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGG729690.1680295971945834Illumina Small RNA Adapter 2 (100% over 21bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC720880.1660008716381358RNA PCR Primer, Index 1 (100% over 24bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA715720.1648126509944048RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATAACGATCTCGTATGC680530.15670926253454187RNA PCR Primer, Index 1 (98% over 50bp)
CGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC680300.1566562992112748RNA PCR Primer, Index 1 (100% over 31bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA667220.1536442980446079No Hit
TTATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTG663530.15279458211914915No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG626180.14419379897121276No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA608670.14016167814335825No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC603470.13896424648688518No Hit
AGAAAAGTTACCATAGGGATAACTGGCTTGTGGAATTCTCGGGTGCCAAG589320.13570585072936714No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAAC585450.13481468524656892RNA PCR Primer, Index 1 (100% over 24bp)
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT580190.13360343707098268RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGA574190.13222178515966762RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA554120.12760015951631867No Hit
TGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC553670.12749653562297003RNA PCR Primer, Index 1 (100% over 31bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC550500.12676656286315857No Hit
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA531950.12249495570400945RNA PCR Primer, Index 1 (100% over 23bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTG514830.11855264225039042No Hit
AGCAGAGTTGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC509830.11740126565762785No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAGCTGGAATTCTCGGGTGCCAA504200.11610481561417721No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA503960.1160495495377246No Hit
AGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAGGAACTCCA493720.11369153027574687RNA PCR Primer, Index 1 (100% over 28bp)
AAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGAACT485440.11178485063813207RNA PCR Primer, Index 1 (100% over 25bp)
TGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT478250.1101291710977395RNA PCR Primer, Index 1 (100% over 30bp)
AGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAGGAACTC473040.10892943668808089RNA PCR Primer, Index 1 (100% over 26bp)
ATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAA465640.1072253993307923RNA PCR Primer, Index 1 (100% over 23bp)
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTG455750.10494797643030793No Hit
AAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG454980.10477066443502249Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGC444580.10237580112207635No Hit
CGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT443090.10203269089743311RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)