FastQCFastQC Report
Tue 18 Jul 2023
SRR10174368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26966897
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC13827355.127527279093327RNA PCR Primer, Index 11 (100% over 50bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC5779422.1431535115070894No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT3601581.3355559595900113RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3190621.183161711189834No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA2997001.1113625716744495No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA2919721.0827052144709122RNA PCR Primer, Index 1 (100% over 22bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA2724571.0103387126817RNA PCR Primer, Index 1 (100% over 23bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG2704871.0030334598748978No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG1847820.6852178802774379Illumina Small RNA Adapter 2 (100% over 21bp)
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA1769680.6562416135605073RNA PCR Primer, Index 1 (100% over 23bp)
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA1730010.6415309851926976RNA PCR Primer, Index 1 (100% over 23bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA1675660.6213766455962656No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA1629750.604352069131276No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC1466740.5439038833426033No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATG1385310.513707602324435RNA PCR Primer, Index 11 (100% over 49bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA1369740.5079338568319521No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA1355450.5026347673594036No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC1344740.49866323144260905No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA1331430.49372755048532285RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1313150.4869488692006351No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG1292510.4792950408791935Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1194660.44300981310530463RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA1187900.4405030360000262No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC1138950.42235115148769253No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA1124270.4169074402590702RNA PCR Primer, Index 1 (100% over 22bp)
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1071710.3974168774405153RNA PCR Primer, Index 1 (100% over 27bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG1004630.37254193539582997Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG970120.35974476410838074Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA925710.34327642516675166No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC891340.33053116938148275RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA879680.3262073496998931No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA862150.31970678717688583No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC849270.31493056097629624No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA847840.31440028120402586RNA PCR Primer, Index 1 (100% over 22bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC837550.3105844917937722No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG784220.29080839371322553No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC714440.2649322241264911No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTGGAATTCTCGGGTGCCA702530.26051569819100806No Hit
TTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAAC620110.22995230040742173RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA580920.2154196680470875No Hit
ATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAA547390.20298590527490054RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG547060.20286353301976123RNA PCR Primer, Index 1 (100% over 29bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG522010.19357436637963946No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGGAATTCTCGGGTGCCAAGGAA493580.1830318111868785RNA PCR Primer, Index 1 (100% over 23bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA487990.18095889934982137No Hit
TTGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT471450.17482545359223198RNA PCR Primer, Index 1 (100% over 25bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCA465620.17266354375143717No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG463360.17182547921624056No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA458670.17008630989319978RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC447710.16602206772251177No Hit
AACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACT445650.16525816819042993RNA PCR Primer, Index 1 (100% over 25bp)
AAAAACGTTCGTAGTTGAACTTGTGCTTCATACGTGGAATTCTCGGGTGC440790.16345595861474163No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG439530.16298871909511875Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA425040.15761546461945547No Hit
ATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG403450.14960935253321878RNA PCR Primer, Index 1 (100% over 29bp)
ATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAACT394400.1462533861422766RNA PCR Primer, Index 1 (100% over 25bp)
AAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC390620.14485166758340792RNA PCR Primer, Index 1 (100% over 27bp)
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGGAATTCTCGGGTGCCA374270.13878867857877752No Hit
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC367150.13614840446789261RNA PCR Primer, Index 1 (100% over 26bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC351700.1304191579772786No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG328810.12193097337079604No Hit
ATTATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAG324820.1204513815586569No Hit
TTTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG322180.11947240351754225Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGT318760.11820418196428013No Hit
AGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAGGAACTCCA313540.11626847538298529RNA PCR Primer, Index 1 (100% over 28bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC308400.11436243480293636RNA PCR Primer, Index 1 (100% over 24bp)
GCGCAGTGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA306350.11360224352101023RNA PCR Primer, Index 1 (100% over 28bp)
AGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAAG303420.11251572622537921No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC302440.11215231771011695No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTTGGAATTCTCGGGT286490.10623765871171607No Hit
TGCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC281200.10427599437933108No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA281100.1042389118777737No Hit
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA280500.10401641686842947RNA PCR Primer, Index 1 (100% over 28bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA277800.1030151893263804No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA275790.10226983104507724RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)