FastQCFastQC Report
Tue 18 Jul 2023
SRR10174369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39112396
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC11276212.8830271610054266No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC8257052.111108202115769RNA PCR Primer, Index 10 (100% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA3961621.0128809291049314No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3865750.9883695184513881No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC3599050.9201814176763806No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA3333340.8522464335859148RNA PCR Primer, Index 1 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG3091700.7904655086842546No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG3047500.7791647435764355No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGCGTATGC2401960.6141173248501575RNA PCR Primer, Index 10 (98% over 50bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA2352290.6014180261418912No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA2254200.5763390204987697RNA PCR Primer, Index 1 (100% over 23bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA2073470.5301311635318889No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC2060860.526907121721717No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT2044600.5227498719331846RNA PCR Primer, Index 1 (100% over 25bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG2024390.5175827121406728Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA2004130.5124027686772245No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1859050.4753096690880303No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC1653460.4227457709315482RNA PCR Primer, Index 1 (100% over 26bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA1617920.4136591376299217RNA PCR Primer, Index 1 (100% over 22bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC1607590.41101803121445185No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1605830.41056804599748886RNA PCR Primer, Index 1 (100% over 27bp)
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1474730.37704926080212525RNA PCR Primer, Index 1 (100% over 30bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG1465350.37465104413444783Illumina Small RNA Adapter 2 (100% over 21bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA1409760.36043815878730623No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC1408230.3600469784566509No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1302660.33305553564143703No Hit
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA1283720.328213081090711RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA1267630.3240992957833624No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1224200.31299539920796465No Hit
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA1134350.29002314253516964RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1114630.284981262717835No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTGGAATTCTCGGGTGCCA1070660.27373930249632367No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC1063130.27181408165329474No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA968810.24769896479878142RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA921290.23554936394078238No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA909490.23253241759978088No Hit
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA903060.23088843751735383RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG867530.2218043609499147RNA PCR Primer, Index 1 (100% over 29bp)
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT837140.2140344457547423RNA PCR Primer, Index 1 (100% over 30bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC834200.21328276590367926No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGG817440.20899767940578223RNA PCR Primer, Index 10 (97% over 49bp)
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG781290.19975508531873118Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC760600.19446520228522948No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA729960.18663136873537484No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG703070.17975631050575372Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTTTGC686640.17555559623603728RNA PCR Primer, Index 10 (98% over 50bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC662200.16930693788230206No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG640330.1637153602147002No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCA638320.16320145664305505No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA637360.16295601016107528No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG616090.1575178365447108No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG612840.15668689793384175Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTCTGGAATTCTCGGGTGCC608000.15544943858719368No Hit
ATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA602420.15402278091068622RNA PCR Primer, Index 1 (100% over 32bp)
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGGAATTCTCGGGTGCCA544020.1390914532569163No Hit
TTGGCAAATGTGAGATGCAGTGTATGGAGCGTCTGGAATTCTCGGGTGCC527690.13491630632907275No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGCGTATGG522330.13354589680468565RNA PCR Primer, Index 10 (97% over 49bp)
TTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAAC502970.12859605941809343RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG501420.1281997656190636Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGCGTTTGC488420.12487601117558741RNA PCR Primer, Index 10 (97% over 46bp)
AGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAAG482890.12346213717001639No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGCC476140.12173634159359606No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA475780.12164429916285363RNA PCR Primer, Index 1 (100% over 22bp)
ACTGGCACGGACTTGGGGAATCCGACTGTCTGGAATTCTCGGGTGCCAAG469700.12008980477698171No Hit
TCCCCTCATGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA463100.11840236021337072RNA PCR Primer, Index 1 (100% over 32bp)
AACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACT459860.11757397833668896RNA PCR Primer, Index 1 (100% over 25bp)
ATTTTGAACGCATATCGCAGTCCATGCTGGAATTCTCGGGTGCCAAGGAA458650.11726461350002695RNA PCR Primer, Index 1 (100% over 23bp)
GCGCAGTGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA456810.11679417440956572RNA PCR Primer, Index 1 (100% over 28bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGT456360.11667912137113769No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA439140.11227642510062538No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG437570.11187501783322096RNA PCR Primer, Index 10 (100% over 49bp)
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC431310.11027450223197782RNA PCR Primer, Index 1 (100% over 31bp)
ACAACCTCAACTCATATGGGACTACCCCCTGATGGAATTCTCGGGTGCCA422600.10804758675484877No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA413240.10565448355554592No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA410030.10483377188142604RNA PCR Primer, Index 1 (100% over 23bp)
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA400540.10240743113768841No Hit
TGGAACGTACCTTGAGCATATATGCTGTGACCCGATGGAATTCTCGGGTG397200.10155348191913376No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCGCGGATGC392550.10036460052204421RNA PCR Primer, Index 10 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)