FastQCFastQC Report
Tue 18 Jul 2023
SRR10174370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26837661
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC16604586.1870443925795175RNA PCR Primer, Index 12 (100% over 50bp)
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4365021.6264532143840702RNA PCR Primer, Index 1 (100% over 30bp)
ATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3213431.197358443420237RNA PCR Primer, Index 1 (100% over 32bp)
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA2282800.8505957355970775RNA PCR Primer, Index 1 (100% over 28bp)
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2143700.7987655854211736RNA PCR Primer, Index 1 (100% over 31bp)
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1952070.7273621944922846RNA PCR Primer, Index 1 (100% over 30bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC1868490.6962193911011842No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1740730.648614646410505No Hit
AGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1708690.636676199166537RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATGTCGTATGC1475270.5497014065420976RNA PCR Primer, Index 12 (98% over 50bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA1364460.5084124134364765No Hit
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG1277150.47587977208595045RNA PCR Primer, Index 1 (100% over 29bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA1254170.46731717790160626RNA PCR Primer, Index 1 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG1183920.4411412753145664No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG1028900.38337916258797666RNA PCR Primer, Index 12 (100% over 49bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA963710.3590886702086296No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA956310.3563313509325571No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG858170.3197633355604276Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC842060.31376057697427506RNA PCR Primer, Index 1 (100% over 27bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC799000.2977159596732368No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA605280.22553381235421374RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA568880.21197078240164074No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC517970.1930011710036877No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC513100.19118655683146157RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA456410.1700632555124681RNA PCR Primer, Index 1 (100% over 32bp)
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTGGAATTCTCGGGTGCCA413190.15395902049735258No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG409540.15259899139496547No Hit
GCGCAGTGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA408070.1520512536468808RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCGCGTATGC401480.1495957490483243RNA PCR Primer, Index 12 (98% over 50bp)
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA370690.13812306519558468No Hit
AGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAGGAACTC364950.13598427970306354RNA PCR Primer, Index 1 (100% over 26bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA361400.13466151167197468No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG345560.12875935797832755Illumina Small RNA Adapter 2 (100% over 21bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG340540.12688885219915402No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG339780.1266056680572871Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC334100.12448923920754494No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA333970.1244407998148572No Hit
AACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACT326310.12158660175340913RNA PCR Primer, Index 1 (100% over 25bp)
AGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCAAGGAACTC305820.11395180824439208RNA PCR Primer, Index 1 (100% over 26bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC304060.1132960133895424RNA PCR Primer, Index 12 (100% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA301770.11244273485681185No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC300950.11213719407216598No Hit
TCCCCTCATGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA298420.11119448896831956RNA PCR Primer, Index 1 (100% over 32bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGT295720.11018844004326606No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG290150.108112998371952No Hit
GAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCCG287690.10719637601801439RNA PCR Primer, Index 12 (100% over 50bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC281080.10473341920519824No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTTGGAATTCTCGGGT270640.10084336336165808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)