FastQCFastQC Report
Tue 18 Jul 2023
SRR10174375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63692380
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC52860228.299300481470468RNA PCR Primer, Index 7 (100% over 50bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA25294833.9714059986453636RNA PCR Primer, Index 1 (100% over 22bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA20043673.146949446699904No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG17693672.7779885129115915Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA11985991.8818561969265397RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCGCGTATGC11473261.801355201360037RNA PCR Primer, Index 7 (98% over 50bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG10802451.6960349102985317No Hit
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA8921951.4007876609415444RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG8262671.297277633525392Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC7779311.2213878646079799No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA7075741.1109241011248128No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA6065420.9522991604333203No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA5611310.8810017776066776No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA5547840.8710366923013397No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA5433320.8530565194768982RNA PCR Primer, Index 1 (100% over 22bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC5433040.853012558174149No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC5376020.8440601528785704No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA4945710.7764994807856136RNA PCR Primer, Index 1 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG4838810.7597156834145623No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC4287160.6731040667659146RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGG4069400.6389147335992155RNA PCR Primer, Index 7 (97% over 49bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA4038410.63404915941279No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTTTGC3978740.6246806917876204RNA PCR Primer, Index 7 (98% over 50bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA2917510.45806264422839904No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC2808300.44091616610966655No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA2674970.4199827357683918No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG2513020.39455583226753344No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCGCGTTTGC2397780.3764626160931653RNA PCR Primer, Index 7 (97% over 46bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC2367830.3717603267455228No Hit
ATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAACT2344010.3680204759187834RNA PCR Primer, Index 1 (100% over 25bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA2308250.362405989539094RNA PCR Primer, Index 1 (100% over 23bp)
AAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC2306620.3621500719552323RNA PCR Primer, Index 1 (100% over 27bp)
TTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAAC2291790.35982169295604904RNA PCR Primer, Index 1 (100% over 24bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC2258560.3546044283476297No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATG2214280.3476522623271418RNA PCR Primer, Index 7 (100% over 49bp)
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA2194730.34458282136732843RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCGCGTATGG2144110.3366352458488755RNA PCR Primer, Index 7 (97% over 49bp)
ATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAA2047850.32152197798229554RNA PCR Primer, Index 1 (100% over 23bp)
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT1996420.31344722869517516RNA PCR Primer, Index 1 (100% over 25bp)
GGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTC1984520.31157887332833223RNA PCR Primer, Index 1 (100% over 26bp)
ATTATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAG1940140.30461100684257675No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1890760.29685811709344195No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG1848350.29019954977345797No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC1696240.2663175720549303No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGGATGC1609840.25275237006373447RNA PCR Primer, Index 7 (98% over 50bp)
GCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAGGA1533100.24070383301738763RNA PCR Primer, Index 1 (100% over 22bp)
TGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAAC1497120.23505480561410957RNA PCR Primer, Index 1 (100% over 24bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTGC1481940.23267147498648974No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1463360.22975432853977193RNA PCR Primer, Index 1 (100% over 27bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1409510.22129962799317596No Hit
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA1401990.22011895300505335RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCGCGGATGC1332620.20922754024892773RNA PCR Primer, Index 7 (97% over 45bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA1283370.20149506110464077RNA PCR Primer, Index 1 (100% over 22bp)
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA1277000.20049494146709546No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCGCGTTTGG1270560.19948383150386276RNA PCR Primer, Index 7 (97% over 46bp)
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA1208270.18970401168868237RNA PCR Primer, Index 1 (100% over 23bp)
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG1197160.18795968999745338No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1168950.18353058874546688No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTTTGG1167640.1833249126504615RNA PCR Primer, Index 7 (97% over 49bp)
GGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACT1119250.1757274575074758RNA PCR Primer, Index 1 (100% over 25bp)
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC1108440.17403023721204955No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAGCTCGTATGC1077740.16921019437490012RNA PCR Primer, Index 7 (98% over 50bp)
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1050360.16491140698463458No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA1018450.15990138851774732No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC1010590.15866733194771493No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG975350.15313448798741702Illumina Small RNA Adapter 2 (100% over 21bp)
CAACCCTCAACCATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG974030.15292724184588485RNA PCR Primer, Index 7 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA973780.15288799068271589No Hit
CTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG948750.14895816422623867No Hit
TAAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCCAAG944950.14836154654607034No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTACTGGAATTCTCGGGTGCCAA928860.14583534168451548No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTGGTATGC924710.1451837723759106RNA PCR Primer, Index 7 (98% over 50bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG919560.1443751984146298Illumina Small RNA Adapter 2 (100% over 21bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA918410.14419464306405255RNA PCR Primer, Index 1 (100% over 23bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG906670.14235140844163777Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACT887590.13935575966858202RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAAC880170.138190785145727RNA PCR Primer, Index 1 (100% over 24bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA805930.12653475973106987No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGAACT795160.12484381962175067RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATGTCGTATGC787820.12369140547110974RNA PCR Primer, Index 7 (98% over 50bp)
AAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG780060.12247304936634491Illumina Small RNA Adapter 2 (100% over 21bp)
ATTTTGAACGCATATCGCAGTCCATGCTGGAATTCTCGGGTGCCAAGGAA762920.11978198961948039RNA PCR Primer, Index 1 (100% over 23bp)
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGC758640.11911000970602763No Hit
AAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCCAAGG742530.11658066475141923Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTTGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC725500.11390687551634905No Hit
TGTTTATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCA715560.11234624926875084No Hit
TCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCCAAG681670.10702536158956534No Hit
AAAATGGAACGTGTATCATTTCCTAGCCTGGAATTCTCGGGTGCCAAGGA676040.10614142539500016RNA PCR Primer, Index 1 (100% over 22bp)
CGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAGG675900.10611944474362553Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA671300.10539722334131649No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTG668350.10493405961592266No Hit
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG657830.10328237066977242RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCGGGTATGC649560.10198394219214292RNA PCR Primer, Index 7 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)