FastQCFastQC Report
Tue 18 Jul 2023
SRR10174376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41691393
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC15253173.6585896758115037RNA PCR Primer, Index 9 (100% over 50bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA14892533.5720874090246877RNA PCR Primer, Index 1 (100% over 22bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG13649393.273910756591894No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA11644282.7929697623679783No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA10368602.4869881416531228No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA9067072.17480620040688RNA PCR Primer, Index 1 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG9046482.169867531171242No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA8829632.117854397429225No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG8101051.9430989029318353Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG7803311.8716836830086248Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC7542591.80914799368781No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC6452921.5477822964562495No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA6354801.5242474627796678No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA5730611.3745307094920047No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC5710011.3695896416797586No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC5027201.2058124323166655No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA4891191.173189391872802No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC4348591.0430426251288845No Hit
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA4093730.9819125017002909RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA3871850.9286928839245069RNA PCR Primer, Index 1 (100% over 22bp)
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT3695620.8864227683637245RNA PCR Primer, Index 1 (100% over 25bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA3461890.8303608373075949No Hit
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA3358130.8054732064241652RNA PCR Primer, Index 1 (100% over 23bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC3251220.7798300239092515No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA3072710.7370130328818708RNA PCR Primer, Index 1 (100% over 22bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA3018210.7239407903688898RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG2580800.6190246509633296No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC2548570.6112940385561116No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG2327380.5582399225662716Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG2004050.48068674510347975Illumina Small RNA Adapter 2 (100% over 21bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1979660.47483661675684474No Hit
TGTTTATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCA1856200.4452237899558789No Hit
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGC1847180.4430602738555654No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC1774900.4257233621337622RNA PCR Primer, Index 1 (100% over 26bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG1701520.4081226069850916No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1661020.3984083717231516No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA1591300.3816854956129674RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC1529000.36674236334583493No Hit
TTATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTG1467010.351873586953547No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1416830.3398375295351729No Hit
TTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAAC1409530.3380865686114158RNA PCR Primer, Index 1 (100% over 24bp)
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA1408420.33782032660794037No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA1323210.31738205533214014No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTGC1270990.30485668828575724No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1268640.30429302278290393No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1261770.30264520065328593No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGG1235770.2964089014727812Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC1218200.29219460237272477No Hit
AAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC1180090.2830536269200696RNA PCR Primer, Index 1 (100% over 27bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTG1159850.27819890786570745No Hit
CTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1131640.2714325232548598No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1103020.26456779700308886No Hit
ATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAACT1054240.2528675403098189RNA PCR Primer, Index 1 (100% over 25bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA1019030.24442215207345075RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGA1016800.2438872694898921RNA PCR Primer, Index 1 (100% over 22bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTG1013380.24306695628999492No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAAC968840.23238369607846876RNA PCR Primer, Index 1 (100% over 24bp)
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC910240.2183280371562543No Hit
AGAAAAGTTACCATAGGGATAACTGGCTTGTGGAATTCTCGGGTGCCAAG856430.20542129642921741No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCAAG807550.19369705396986855No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGAACT806780.1935123635710613RNA PCR Primer, Index 1 (100% over 25bp)
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGGAATTCTCGGGTGCCA796610.1910730111608408No Hit
TTGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT789310.1893220502370837RNA PCR Primer, Index 1 (100% over 25bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATG776580.18626866221524427RNA PCR Primer, Index 9 (100% over 49bp)
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTG768660.18436898954179823No Hit
TCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCCAAG728660.17477468311025252No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG717620.1721266545351459Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG709970.17029174343011277Illumina Small RNA Adapter 2 (100% over 21bp)
GGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTC705810.16929393556123204RNA PCR Primer, Index 1 (100% over 26bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA704480.16897492487238314RNA PCR Primer, Index 1 (100% over 23bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC663210.15907599921163584RNA PCR Primer, Index 1 (100% over 24bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA655020.15711156496977685No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCA636020.15255426941479264No Hit
AGCAGAGTTGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC621190.14899718030529704No Hit
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGG606180.14539691681685954Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTACTGGAATTCTCGGGTGCCAA600530.1440417210334037No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA579900.139093457491334No Hit
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG577210.13844824038381254No Hit
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC571640.1371122332132198RNA PCR Primer, Index 1 (100% over 26bp)
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA568550.1363710730413829RNA PCR Primer, Index 1 (100% over 28bp)
TTAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTG565890.13573305166368513No Hit
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA555700.13328890210024885RNA PCR Primer, Index 1 (100% over 23bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGAA545230.13077759239179176RNA PCR Primer, Index 1 (100% over 23bp)
AAGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGC518510.12436859569551922No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTGGAATTCTCGGGTGCCAA512970.12303978425475014No Hit
ATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAA477770.1145967945949899RNA PCR Primer, Index 1 (100% over 23bp)
AAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG476730.11434734262776973Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAAC475650.11408829635411798RNA PCR Primer, Index 1 (100% over 24bp)
AAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA467660.11217183364441673No Hit
CTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA466840.11197515036257004No Hit
TAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTC466370.11186241726199937RNA PCR Primer, Index 1 (100% over 26bp)
TAGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCC458990.1100922677253792No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA442690.10618258785452432No Hit
TATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA439160.1053358903119404No Hit
TATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTGC436950.1048058048815975No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG432020.10362330661390949Illumina Small RNA Adapter 2 (100% over 21bp)
ATTATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAG424040.10170924248081613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)