FastQCFastQC Report
Tue 18 Jul 2023
SRR10174377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49155765
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA21486424.371088518305025No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT18397493.742692235590271RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC16567793.37046732972216RNA PCR Primer, Index 8 (100% over 50bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG15376473.128111219508027No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC9218021.8752673262230786No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG9106941.852669773321603Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA7902681.607681215011098RNA PCR Primer, Index 1 (100% over 22bp)
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC6967921.4175183724635352RNA PCR Primer, Index 1 (100% over 26bp)
TTGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT6645361.3518983988958364RNA PCR Primer, Index 1 (100% over 25bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA5934571.2072988793888164RNA PCR Primer, Index 1 (100% over 22bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG5697901.159151932636996No Hit
ATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAACT5558761.1308459953781616RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGTATGC5497331.118348987143217RNA PCR Primer, Index 8 (98% over 50bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA4565480.9287781402649313RNA PCR Primer, Index 1 (100% over 23bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC4203030.8550431470245657No Hit
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA4188210.8520282412449487RNA PCR Primer, Index 1 (100% over 28bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC4068310.8276363921912312No Hit
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA3963660.8063469259404263RNA PCR Primer, Index 1 (100% over 23bp)
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3913610.7961650072987371RNA PCR Primer, Index 1 (100% over 30bp)
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA3834150.7800000671335295RNA PCR Primer, Index 1 (100% over 23bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC3821390.777404237325978No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC3430900.6979649284270115No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG3344840.6804573176716913Illumina Small RNA Adapter 2 (100% over 21bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA3316440.6746797654354479No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG3171370.645167458994891Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAAC2984850.6072227743785495RNA PCR Primer, Index 1 (100% over 24bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA2843360.5784387650156599No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA2801910.5700063868398753No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG2510950.5108149573096868Illumina Small RNA Adapter 2 (100% over 21bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG2505430.5096919964525015No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA2502470.5090898290363297RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG2451730.4987675402874922No Hit
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG2432490.4948534520823753RNA PCR Primer, Index 1 (100% over 29bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC2406780.4896231398290719RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGG2311120.470162553669951RNA PCR Primer, Index 8 (97% over 49bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG2154890.438379913322476No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA1951750.3970541400383048No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA1859030.37819165259659776No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1797320.3656376825790424No Hit
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1789440.36403461526842273RNA PCR Primer, Index 1 (100% over 31bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTTTGC1723800.3506811459449365RNA PCR Primer, Index 8 (98% over 50bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC1669230.3395797013839577No Hit
ATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1536960.31267136214846825RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGTATGG1528800.31101133305523776RNA PCR Primer, Index 8 (97% over 49bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA1326650.2698869603595835RNA PCR Primer, Index 1 (100% over 22bp)
TTATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTG1324980.26954722401329734No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGGATGC1281760.26075476599743697RNA PCR Primer, Index 8 (97% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGTTTGC1269180.2581955544787066RNA PCR Primer, Index 8 (97% over 46bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1237790.25180973177815463No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1236850.2516185029365325RNA PCR Primer, Index 1 (100% over 27bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC1232980.2508312097268754No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG1211760.24651432034472456Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGGATGC1181940.24044789049666912RNA PCR Primer, Index 8 (98% over 50bp)
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG1179980.24004915801839316No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA1177260.23949581498731634RNA PCR Primer, Index 1 (100% over 22bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA1148330.23361044223398822RNA PCR Primer, Index 1 (100% over 23bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA1118960.2276355581079859No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC1107740.22535301810479402No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA1085420.22081235029095775RNA PCR Primer, Index 1 (100% over 23bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA1082900.2202996942474601No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA1062530.21615572456252077No Hit
AAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1041640.21190596870987563RNA PCR Primer, Index 1 (100% over 33bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCAAG955680.19441870144834486No Hit
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC933200.18984548404444523RNA PCR Primer, Index 1 (100% over 24bp)
TCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCCAAG902970.18369564587185247No Hit
AAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC833010.16946333761665597RNA PCR Primer, Index 1 (100% over 27bp)
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA809000.1645788647577756RNA PCR Primer, Index 1 (100% over 23bp)
ATGGGACGTGTGCTTTTATTAGGCTGGAATTCTCGGGTGCCAAGGAACTC802110.16317719803567293RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGTTTGG772970.15724910394538666RNA PCR Primer, Index 8 (97% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGGGGTTGG762850.15519034237388027RNA PCR Primer, Index 8 (97% over 42bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC756600.15391887401203094No Hit
TAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTC740520.150647640210665RNA PCR Primer, Index 1 (100% over 26bp)
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGGAATTCTCGGGTGCCA734810.1494860267152795No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTGGTATGC721040.14668472762045306RNA PCR Primer, Index 8 (98% over 50bp)
TATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAAC704970.1434155281684661RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATTC696540.14170057164200375RNA PCR Primer, Index 8 (98% over 50bp)
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT691970.14077087397581953RNA PCR Primer, Index 1 (100% over 30bp)
TACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCA688470.1400588516931839No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGGATGG684100.13916984101457885RNA PCR Primer, Index 8 (97% over 45bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA657640.1337869525578536No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA650630.1323608736432034No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGGGTATGC650120.1322571218248765RNA PCR Primer, Index 8 (97% over 42bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATG632980.12877024698934092RNA PCR Primer, Index 8 (100% over 49bp)
TAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA627960.12774900360110356RNA PCR Primer, Index 1 (100% over 32bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTG627510.1276574578790504No Hit
TTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCCAAGGA625470.12724245060574277RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTTTGG617050.1255295284286594RNA PCR Primer, Index 8 (97% over 49bp)
TGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAAC610890.1242763692112207RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGGGTTTGG604550.12298659170496076RNA PCR Primer, Index 8 (97% over 42bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA602730.12261634011799025No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAAC599940.12204875664126068RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA597950.12164392111484787No Hit
ATGTATGTAAGCGTATTACCGGTGTGGAATTCTCGGGTGCCAAGGAACTC583200.11864325578088347RNA PCR Primer, Index 1 (100% over 26bp)
TGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACT577170.11741654310537127RNA PCR Primer, Index 1 (100% over 25bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTGC575970.1171724211798962No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC568650.11568327743449827No Hit
TTATGTATGTAAGCGTATTACCGGTGTGGAATTCTCGGGTGCCAAGGAAC538180.10948461487681048RNA PCR Primer, Index 1 (100% over 24bp)
ATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAA531680.1081622877804872RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGGGTATGG508950.1035382116421136RNA PCR Primer, Index 8 (97% over 42bp)
AGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC504750.10268378490295084RNA PCR Primer, Index 1 (100% over 27bp)
TATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTGC502840.1022952241715697No Hit
GTCTAGGGGTATGATTTCCGCTTTGGAATTCTCGGGTGCCAAGGAACTCC500920.1019046290908096RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)