FastQCFastQC Report
Tue 18 Jul 2023
SRR10174378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83368394
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA34324784.1172413612765535No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG31658043.7973671413173675No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC30431603.650256234994763RNA PCR Primer, Index 6 (100% over 50bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA23950012.8727925357420223RNA PCR Primer, Index 1 (100% over 22bp)
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA19421912.3296490514138966RNA PCR Primer, Index 1 (100% over 28bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG18602602.2313731988168084Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG14478111.7366425458549675Illumina Small RNA Adapter 2 (100% over 21bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG14442421.7323615469910576No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC14295891.7147853417927184No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA11840351.420244463387408RNA PCR Primer, Index 1 (100% over 22bp)
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT10519991.2618678968434969RNA PCR Primer, Index 1 (100% over 30bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA9730891.1672157196646968RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG9511651.140917983858487RNA PCR Primer, Index 1 (100% over 29bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA8765301.0513936492527372No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC8755861.0502613256529807No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC8300250.995611118525325No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA7730220.9272362857319765No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC7676790.9208273821371682No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC7598420.9114269371675794No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA6838150.820232905050324No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA6377760.7650093391507578No Hit
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA6284460.7538180476404523RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA5994950.7190914580890212No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC5875440.7047562892959172No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC4618780.5540205080596851RNA PCR Primer, Index 1 (100% over 26bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA4612310.55324443457553RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG4533030.5437348355301171No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG4439070.5324643773274558No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG4401400.5279458783864782No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA3790060.45461593034885617No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG3747440.44950368121520967No Hit
AAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC3740810.4487084158056349RNA PCR Primer, Index 1 (100% over 27bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC3393870.4070931245239053No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG3335710.40011685963388Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT3125530.3749058666045552RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA2885330.3460939885683776No Hit
AACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAAC2812340.33733887209102287RNA PCR Primer, Index 1 (100% over 24bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG2654930.3184576159641506Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC2622610.314580847029391No Hit
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA2448310.29367364327541207RNA PCR Primer, Index 1 (100% over 23bp)
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2384430.28601126705163593RNA PCR Primer, Index 1 (100% over 31bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA2340130.28069750270108357RNA PCR Primer, Index 1 (100% over 23bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA2332010.2797235124860388No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATG2299040.275768776354262RNA PCR Primer, Index 6 (100% over 49bp)
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC2259270.27099838339215215RNA PCR Primer, Index 1 (100% over 27bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG2231820.2677057686873517Illumina Small RNA Adapter 2 (100% over 21bp)
GGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTC2215100.2657002124810033RNA PCR Primer, Index 1 (100% over 26bp)
TTATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTG2214800.2656642276208415No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA2206400.26465665153631246RNA PCR Primer, Index 1 (100% over 22bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA2161240.25923973058662975RNA PCR Primer, Index 1 (100% over 22bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCAAG2077560.24920235359217785No Hit
TCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCCAAG2046860.24551990290229173No Hit
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG1982610.23781314535098277No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA1876250.22505531292830228No Hit
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA1775680.2129919883067437RNA PCR Primer, Index 1 (100% over 23bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTG1689180.2026163536267713No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG1573200.18870460668823727Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC1554190.1864243660493208No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA1493070.17909305053903282RNA PCR Primer, Index 1 (100% over 23bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA1444070.17321552337927967No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTACTGGAATTCTCGGGTGCCAA1443150.17310516980811697No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTGC1440240.17275611666454796No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT1376990.16516930864711152RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1375440.16498338686960912No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1323560.15876040505230316No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA1313910.1576028920504334No Hit
AACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACT1307070.1567824372387454RNA PCR Primer, Index 1 (100% over 25bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGCC1296580.15552416662842275No Hit
TACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCA1250020.14993931633131855No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1241100.14886936648917576No Hit
CTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1210030.14514253447175676No Hit
AGAAAAGTTACCATAGGGATAACTGGCTTGTGGAATTCTCGGGTGCCAAG1192490.1430386196476329No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAGCTGGAATTCTCGGGTGCCAA1190860.1428431019074207No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGAACT1166370.13990553782288287RNA PCR Primer, Index 1 (100% over 25bp)
TGTTTATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCA1111560.13333110387133043No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA1074650.12890376657609595No Hit
AGCAGAGTTGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC1062610.12745957418827092No Hit
AAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA1060760.12723766755060678No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTG1045800.12544322252387397No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG1026600.12314019147352173No Hit
ATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAA1019720.12231493868047885RNA PCR Primer, Index 1 (100% over 23bp)
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGC1004940.1205420845698431No Hit
CGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC988650.11858810666305986RNA PCR Primer, Index 1 (100% over 31bp)
TGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA942430.1130440392074723RNA PCR Primer, Index 1 (100% over 22bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCC927520.11125559165743315No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG926380.11111884918881848Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGC916260.10990495990602865No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCA915780.10984738412976985No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGGAATTCTCGGGTGCCAAGGAA910430.10920565412355189RNA PCR Primer, Index 1 (100% over 23bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG910250.10918406320745484Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGG890380.10680066596940802Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA852330.10223658620555892No Hit
ATTATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAG834340.1000786940911924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)