FastQCFastQC Report
Tue 18 Jul 2023
SRR10174379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83683932
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC2474707729.57207722983189RNA PCR Primer, Index 4 (100% over 50bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA23309322.7853997109026856No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG14977881.7898155167947891RNA PCR Primer, Index 4 (100% over 49bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA12465391.4895798634318473RNA PCR Primer, Index 1 (100% over 22bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG12136631.4502939465129339Illumina Small RNA Adapter 2 (100% over 21bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG8858191.0585293721619105No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT8445531.0092176357105207RNA PCR Primer, Index 1 (100% over 25bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA8262220.987312594250471RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA7731560.9239001819369578RNA PCR Primer, Index 1 (100% over 22bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC6947000.8301474170692649RNA PCR Primer, Index 4 (100% over 50bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC5444930.6506541781521451No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG5244490.6267021487470259Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC5039540.6022111867305662No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA4300450.5138919619599136No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG4264660.5096151552725797No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC4135400.49416894034090086No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG3906630.46683155375634117No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA3869380.4623802810795268No Hit
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA3811590.4554745348246782RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA3784570.452245719046758No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG3770800.450600241872RNA PCR Primer, Index 4 (100% over 49bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC3757710.44903602282932886No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA3371920.4029351775678992No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3353210.40069938396298105No Hit
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA3340480.3991781839314147RNA PCR Primer, Index 1 (100% over 23bp)
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC3225110.3853917858448621RNA PCR Primer, Index 1 (100% over 26bp)
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG3115350.37227576734802564RNA PCR Primer, Index 1 (100% over 29bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA3114270.3721467103147113RNA PCR Primer, Index 1 (100% over 22bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA3092620.36955959478577083No Hit
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA2901560.34672844961443733RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAGCAATCTCGTATGC2871810.3431734063356392RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATATCGTATGC2863270.34215289979443125RNA PCR Primer, Index 4 (98% over 50bp)
ATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAACT2781670.3324019239440135RNA PCR Primer, Index 1 (100% over 25bp)
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2698570.3224717022139925RNA PCR Primer, Index 1 (100% over 30bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC2670070.31906603050153043No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACAATCTCGTATGCC2532520.30262918334191086RNA PCR Primer, Index 4 (97% over 39bp)
ACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2513920.3004065344348303RNA PCR Primer, Index 3 (100% over 34bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG2512930.3002882321542922Illumina Small RNA Adapter 2 (100% over 21bp)
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG2437700.291298453805923Illumina Small RNA Adapter 2 (100% over 21bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG2404520.28733353494909875No Hit
TTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAAC2374540.28375100730209474RNA PCR Primer, Index 1 (100% over 24bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA2310150.27605657917699183RNA PCR Primer, Index 1 (100% over 22bp)
GAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCCG2293270.2740394655451897RNA PCR Primer, Index 4 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCCCAATCTCGTATGC2165070.2587199177017638RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACAAATCTCGTATGC2137320.2554038689291034RNA PCR Primer, Index 4 (98% over 50bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG2117850.253077257411853Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC2024360.2419054592224467RNA PCR Primer, Index 1 (100% over 27bp)
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2016260.24093753147258903RNA PCR Primer, Index 1 (100% over 31bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA2010050.24019545353103153RNA PCR Primer, Index 1 (100% over 23bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC1925400.23008001105875378No Hit
TTGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT1921650.2296318963597456RNA PCR Primer, Index 1 (100% over 25bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1863140.22264011208268752No Hit
CGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTAT1847190.22073413089623944RNA PCR Primer, Index 4 (100% over 48bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACGAATCTCGTATGC1769440.21144321947013678RNA PCR Primer, Index 4 (98% over 50bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC1751810.20933648289853304RNA PCR Primer, Index 1 (100% over 26bp)
AAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1717990.2052950857997447RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA1709980.20433791280266322No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA1680450.20080915891954026RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAACCAATCTCGTATGC1644050.1964594589078343RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCAAGTCACTGACCAATCTCGTATGC1640190.19599819951098854RNA PCR Primer, Index 4 (98% over 50bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1554200.1857226307195986No Hit
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC1541870.18424922958925974RNA PCR Primer, Index 1 (100% over 24bp)
ATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1535520.1834904220322726RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAACAATCTCGTATGC1523150.18201224101181096RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTACAGTCACTGACCAATCTCGTATGC1521010.18175651689024364RNA PCR Primer, Index 4 (98% over 50bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA1508740.18029028559508892RNA PCR Primer, Index 1 (100% over 23bp)
CAACCCTCAACCATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA1412260.16876119061900677RNA PCR Primer, Index 4 (100% over 36bp)
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1400510.16735709789544784RNA PCR Primer, Index 1 (100% over 30bp)
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG1383680.16534595912629918No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAGCTCGTATGC1329500.15887159795502917RNA PCR Primer, Index 4 (98% over 50bp)
ATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1243350.14857690960314818RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCAGTCACTGACCAATCTCGTATGCC1204610.14394758601926114TruSeq Adapter, Index 4 (96% over 26bp)
TCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCCAAG1170550.13987750957973627No Hit
CGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1141420.13639655459784084RNA PCR Primer, Index 1 (100% over 31bp)
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1140830.13632605121853023RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGACACTGACCAATCTCGTATGC1140610.13629976182285508RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGACCAATCTCGTATGC1139150.13612529583337454RNA PCR Primer, Index 4 (98% over 50bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1137860.13597114437691574RNA PCR Primer, Index 4 (98% over 50bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG1117860.13358119931553886Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCAAG1072310.128138099438253No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA1054590.12602060811387303No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC1009490.12063128200046816No Hit
GGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTC996750.11910888699637105RNA PCR Primer, Index 1 (100% over 26bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC954710.11408522247735682No Hit
TGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAAC938580.11215773178535635RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACTGACCAATCTCGTATGCC932980.11148854716817083RNA PCR Primer, Index 4 (97% over 35bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAAC922770.11026848021433791RNA PCR Primer, Index 1 (100% over 24bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG896340.10711016781572835No Hit
TGGAATTCTAGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC872210.10422669909917713RNA PCR Primer, Index 4 (98% over 50bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA847820.101312161096828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)