FastQCFastQC Report
Tue 18 Jul 2023
SRR10174380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37912988
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC417443511.010567143903298RNA PCR Primer, Index 3 (100% over 50bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA14018353.6975059839651783No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG8556542.256888852970386No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG8225922.1696839088493896Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA6129601.6167546593795243RNA PCR Primer, Index 1 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC6085191.6050409954498972No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG5300141.3979747520823207No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA4721661.245393794865232RNA PCR Primer, Index 1 (100% over 22bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA4243831.1193604682384835RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA4187791.1045792539485413RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA3864411.0192839456494434No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA3424330.903207629005659No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC3251320.85757419067049No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC3248150.8567380655937749No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA3188740.841067973856347RNA PCR Primer, Index 1 (100% over 22bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG3158720.8331498430036693Illumina Small RNA Adapter 2 (100% over 21bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC3128550.8251921478729137No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3013200.7947672180309291No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG2987040.7878672079341253No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG2900580.7650623580499643Illumina Small RNA Adapter 2 (100% over 21bp)
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG2741180.7230187185457395Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG2529680.667233086455755RNA PCR Primer, Index 1 (100% over 29bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA2479680.6540449937630871No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC2457750.6482606963080831No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA2336730.6163402367547501No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT2256960.595299953672868RNA PCR Primer, Index 1 (100% over 25bp)
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2174380.5735184997816579RNA PCR Primer, Index 1 (100% over 30bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC2091690.5517080320865241RNA PCR Primer, Index 1 (100% over 26bp)
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA1940830.5119169188142069No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG1924720.5076677153486293RNA PCR Primer, Index 3 (100% over 49bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC1912600.5044709216799267No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1890770.4987130004103079No Hit
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA1825970.4816212322806105RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1739560.45882957048914214No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG1450840.3826762480446015Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC1436260.3788306002154196RNA PCR Primer, Index 1 (100% over 27bp)
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA1401900.3697677429170183RNA PCR Primer, Index 1 (100% over 23bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA1355070.3574157753010657RNA PCR Primer, Index 1 (100% over 22bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA1248800.32938580309206966RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA1228660.3240736393554631No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1191940.3143883040819679RNA PCR Primer, Index 1 (100% over 27bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1191880.3143724783707367No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA1096670.28925971226535874RNA PCR Primer, Index 1 (100% over 23bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC1065990.28116749858913787No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGGATCTCGTATGC1029540.27155337901618304RNA PCR Primer, Index 3 (98% over 50bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCAAG1003470.2646771074862261No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG942380.24856389583432464RNA PCR Primer, Index 3 (100% over 49bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC926900.24448086233667474No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG880510.2322449499364176Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGAATCTCGTATGC875170.23083646163684066RNA PCR Primer, Index 3 (98% over 50bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC869300.22928817955472144RNA PCR Primer, Index 1 (100% over 24bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA859620.22673496480942099RNA PCR Primer, Index 1 (100% over 23bp)
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG854360.22534757745815234No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA843180.2223987199320718No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA809750.21358116115775416No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA783520.20666268773118068No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGGAATTCTCGGGTGCCA730220.19260418092079684No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA724330.1910506236016006No Hit
TGGAATTCTCGGGTGCCAAGGAACTACAGTCACTTAGGCATCTCGTATGC723940.1909477564785978RNA PCR Primer, Index 3 (98% over 50bp)
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT723490.19082906364436378RNA PCR Primer, Index 1 (100% over 30bp)
AGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAGGAACTCCA706610.18637676355131916RNA PCR Primer, Index 1 (100% over 28bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC700870.1848627705102009RNA PCR Primer, Index 3 (100% over 50bp)
TTATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTG699870.18459900865634754No Hit
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA674920.17801815040270633RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCGCGTATGC661900.17458397106553564RNA PCR Primer, Index 3 (98% over 50bp)
ATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAACT647550.17079898846274RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAAGCATCTCGTATGC635800.16769978667996308RNA PCR Primer, Index 3 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGACATCTCGTATGC622830.16427879543548507RNA PCR Primer, Index 3 (98% over 50bp)
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC620920.16377501029462516RNA PCR Primer, Index 1 (100% over 31bp)
TCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCCAAG589900.15559311758809408No Hit
TGTTTATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCA559370.14754046818995115No Hit
GCCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA552600.14575480043936395No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAGCTCGTATGC544970.14374229749446285RNA PCR Primer, Index 3 (98% over 50bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA539910.14240766251396486No Hit
TGGAATTCTCGGGTGCCAAGGAACTCAAGTCACTTAGGCATCTCGTATGC536860.14160318885971213RNA PCR Primer, Index 3 (98% over 50bp)
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG529980.13978850730520107No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA525520.1386121294370151No Hit
AGAAAAGTTACCATAGGGATAACTGGCTTGTGGAATTCTCGGGTGCCAAG514130.13560788192162537No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC511670.13495902776114613No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC498020.13135867845604782No Hit
GGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTC493480.1301611996395536RNA PCR Primer, Index 1 (100% over 26bp)
AACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAAC486420.12829904095134892RNA PCR Primer, Index 1 (100% over 24bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTGC480280.12667954316868932No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG454650.11991932685442783No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAGGCATCTCGTATGC454470.11987184972073422RNA PCR Primer, Index 3 (98% over 50bp)
CTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG445540.11751645636582378No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGC439860.1160182890359367No Hit
ATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG435070.11475486975597914RNA PCR Primer, Index 1 (100% over 29bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGCTGGAATTCTCGGGTGCCAA422960.111560713705815No Hit
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGC418280.1103263082297813No Hit
TGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAAC417050.11000188114954168RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAATCACTTAGGCATCTCGTATGC415630.10962733931706992RNA PCR Primer, Index 3 (98% over 50bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCAA408940.10786277251479098No Hit
GATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGAA403380.1063962566073663RNA PCR Primer, Index 1 (100% over 23bp)
TTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAAC403200.1063487794736727RNA PCR Primer, Index 1 (100% over 24bp)
AAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA392940.1036425828531373No Hit
ACCAAGCGTTGGATTGTTCACCCATGCAAGGGTGGAATTCTCGGGTGCCA391520.10326804102066553No Hit
TACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCA390860.10309395819712232No Hit
ATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAA389780.10280909539496069RNA PCR Primer, Index 1 (100% over 23bp)
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC387710.10226310835748426RNA PCR Primer, Index 1 (100% over 26bp)
TGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA385470.10167228180485274RNA PCR Primer, Index 1 (100% over 22bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTACTGGAATTCTCGGGTGCCAA385040.1015588642076958No Hit
AGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG384560.1014322585178462RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)