FastQCFastQC Report
Tue 18 Jul 2023
SRR10174381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34313653
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC531681915.494762390935177RNA PCR Primer, Index 5 (100% over 50bp)
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA17576115.122191449566737RNA PCR Primer, Index 1 (100% over 28bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA12135733.5367059286867537No Hit
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT11766243.429025758347559RNA PCR Primer, Index 1 (100% over 30bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG7809172.275820064975303No Hit
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG6625301.930805793250867RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATGTCGTATGC5938721.7307163419761808RNA PCR Primer, Index 5 (98% over 50bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG4136551.2055114038718058Illumina Small RNA Adapter 2 (100% over 21bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC3737681.0892690440158033No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA3623651.0560373738115263RNA PCR Primer, Index 1 (100% over 22bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA3204710.9339460301705562RNA PCR Primer, Index 1 (100% over 22bp)
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA2764790.8057405021843638RNA PCR Primer, Index 1 (100% over 23bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG2595270.756337426388266Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2044600.5958561159314633RNA PCR Primer, Index 1 (100% over 31bp)
AAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC1890080.5508244779417686RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1825390.5319719238286871No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC1748490.5095610193411935RNA PCR Primer, Index 1 (100% over 26bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATG1708750.4979796234460959RNA PCR Primer, Index 5 (100% over 49bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC1681190.48994783504979783No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1507960.43946355696958295No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG1455910.4242946677813639No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA1438640.4192616857202584No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC1377980.40158359123116394No Hit
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1346090.39228991445475075RNA PCR Primer, Index 1 (100% over 30bp)
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC1290550.3761039374035752No Hit
ATGGGACTACCCCCTGAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1261920.36776031977708695RNA PCR Primer, Index 1 (100% over 29bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC1177720.34322198222381045No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA1156230.33695916899317013RNA PCR Primer, Index 1 (100% over 23bp)
AACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAAC1151720.33564482335937823RNA PCR Primer, Index 1 (100% over 24bp)
ATATGGGACTACCCCCTGAATTTTGGAATTCTCGGGTGCCAAGGAACTCC1147220.3343333920174573RNA PCR Primer, Index 1 (100% over 27bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG1110700.32369039810480105No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1085140.31624146808269RNA PCR Primer, Index 1 (100% over 27bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1018260.29675068404987365No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC1009430.2941773643278377RNA PCR Primer, Index 5 (100% over 50bp)
AGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAGGAACTCCA951340.2772482428495736RNA PCR Primer, Index 1 (100% over 28bp)
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG918010.26753490804374574Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCGCGTATGC891180.2597158629540259RNA PCR Primer, Index 5 (98% over 50bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA863930.2517744176057268No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG841200.2451502321830905Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA819790.23891073328741771RNA PCR Primer, Index 1 (100% over 22bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG783840.2284338540114047Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA749110.2183125183436459No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATG748610.21816680375009914RNA PCR Primer, Index 5 (100% over 49bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC740450.21578874158341577No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC733490.21376039444124473No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC708600.20650672197448636No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA693770.2021848271298891No Hit
TTATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTG682800.1989878489474729No Hit
GGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTC682400.19887127727263548RNA PCR Primer, Index 1 (100% over 26bp)
TTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA650670.1896242291661573RNA PCR Primer, Index 1 (100% over 23bp)
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG643270.18746765318166506No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATGTCGTATG634370.1848739334165325RNA PCR Primer, Index 5 (97% over 49bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA621270.18105621106560704RNA PCR Primer, Index 1 (100% over 22bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA615160.17927557873246547RNA PCR Primer, Index 1 (100% over 22bp)
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG610980.17805740473041445No Hit
TATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAG609040.17749203210745298RNA PCR Primer, Index 1 (100% over 29bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA601270.17522762732373612RNA PCR Primer, Index 1 (100% over 23bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC588540.17151773377203527No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA585700.17069007488068963No Hit
TGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA549110.1600266809249368RNA PCR Primer, Index 1 (100% over 22bp)
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT539450.15721147497761315RNA PCR Primer, Index 1 (100% over 25bp)
TAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTC532010.15504324182563717RNA PCR Primer, Index 1 (100% over 26bp)
AAGTGGAGCCGTACCTGTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA508560.1482092273882935RNA PCR Primer, Index 1 (100% over 28bp)
AACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACT501400.14612259440870373RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA473710.13805292021808344No Hit
GAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCCG447490.1304116469324907RNA PCR Primer, Index 5 (100% over 50bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATGTCGTATGCC445930.12995701740062476RNA PCR Primer, Index 5 (98% over 50bp)
TATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCC445470.12982295997456172RNA PCR Primer, Index 1 (100% over 27bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGT431820.12584495157073483No Hit
ATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA412550.12022911113544221RNA PCR Primer, Index 1 (100% over 32bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCA405640.11821533545262582No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCAAG397490.11584018757781342No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTGGAATTCTCGGGTGCCA396680.11560412993626763No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA387470.1129200671231361No Hit
TACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCA383200.11167566449424664No Hit
AGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG380620.11092377719154531RNA PCR Primer, Index 1 (100% over 29bp)
AGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC375290.1093704596243367RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAGCTCGTATGC374110.10902657318356632RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATGGCGTATGC369120.10757234153996953RNA PCR Primer, Index 5 (97% over 41bp)
TGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA368110.10727799806100505RNA PCR Primer, Index 1 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTTGGAATTCTCGGGT366330.10675925410797854No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCC366080.10668639681120513No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA361520.10535747971805859No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGGAATTCTCGGGTGCCAAGGAA355390.10357101880117514RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGCTCTCGTATGC355160.10350399008814362RNA PCR Primer, Index 5 (98% over 50bp)
AAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGAACT353690.10307558918311611RNA PCR Primer, Index 1 (100% over 25bp)
GATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGAA350630.10218381587060987RNA PCR Primer, Index 1 (100% over 23bp)
ATCACGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC349590.1018807295160326RNA PCR Primer, Index 5 (100% over 42bp)
CAACCCTCAACCATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA343990.10024872606830873RNA PCR Primer, Index 5 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)