FastQCFastQC Report
Tue 18 Jul 2023
SRR10174382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10174382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57951230
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC46285557.986983192591425RNA PCR Primer, Index 2 (100% over 50bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA36854486.359568209337403No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG27307814.712205418245652No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC21314533.678011666016407No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC11431881.97267253861566No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG8738741.507947286019641Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC6297411.0866740878493863No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG5923341.0221249833696369No Hit
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA5642890.973730842296186RNA PCR Primer, Index 1 (100% over 28bp)
ATTATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAG5598390.9660519716320084No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA5116880.882963139867782RNA PCR Primer, Index 1 (100% over 22bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC5035120.868854724912655No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA4411560.761253902635026RNA PCR Primer, Index 1 (100% over 22bp)
TTATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTG3925200.6773281602478498No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGC3909610.6746379671320177No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA3578950.6175796441248961No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA3525890.6084236693509352No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3327370.5741672782441374No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG3251890.5611425331265618Illumina Small RNA Adapter 2 (100% over 21bp)
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG3219130.5554895038465966Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG3088270.5329084473271749RNA PCR Primer, Index 1 (100% over 29bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA3026380.5222287775427717RNA PCR Primer, Index 1 (100% over 22bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG2966320.5118648905295021No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG2828180.4880276052811994No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA2603410.4492415432769934No Hit
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2527210.43609255575766037RNA PCR Primer, Index 1 (100% over 30bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC2170980.37462190189923494No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA2020630.3486776725877949No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG2013540.3474542300482665Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA1984130.34237927305425614RNA PCR Primer, Index 1 (100% over 23bp)
AAAAACGTTCGTAGTTGAACTTGTGCTTCATACGTGGAATTCTCGGGTGC1975950.3409677413231781No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA1941930.3350972878401373No Hit
TATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTGC1928400.33276256604044474No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1883800.32506643948713426No Hit
CCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAG1866290.32204493329994893No Hit
TAAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCCAAG1797590.3101901374655896No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG1616110.278874149867052Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATG1504440.25960449847224987RNA PCR Primer, Index 2 (100% over 49bp)
TCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCCAAG1486690.25654157815114537No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA1483930.25606531561107504No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG1473180.25421030752927937Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCCAAGG1473170.25420858194036605Illumina Small RNA Adapter 2 (100% over 21bp)
ATATCAGTTATGGTTCCTTAGATCGTTTGGAATTCTCGGGTGCCAAGGAA1426040.24607588139199116RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCGCGTATGC1419580.24496115095400048RNA PCR Primer, Index 2 (98% over 50bp)
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGGAATTCTCGGGTGCCA1408630.2430716310939388No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1374520.23718564731067832No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGATGTATCTCGTATGC1374420.23716839142154533RNA PCR Primer, Index 2 (98% over 50bp)
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA1372070.23676287802692023RNA PCR Primer, Index 1 (100% over 23bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC1363960.2353634254182353RNA PCR Primer, Index 1 (100% over 26bp)
AGAAAAGTTACCATAGGGATAACTGGCTTGTGGAATTCTCGGGTGCCAAG1305960.22535500972110512No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCC1225720.21150888428079956No Hit
TGGAATTCTCGGGTGCCAAGGAACTACAGTCACCGATGTATCTCGTATGC1192090.20570572876537738RNA PCR Primer, Index 2 (98% over 50bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC1184030.20431490410125894No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA1153970.19912778382788424No Hit
TACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCA1108600.19129878692824984No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGGA1051050.1813680227322181RNA PCR Primer, Index 1 (100% over 22bp)
ATCCGTGCTGGACTGCAATGATAAATAAGGGGCTGGAATTCTCGGGTGCC1022570.17645354550714454No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA968040.16704390916292888No Hit
AACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAAC961520.1659188251914584RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA956130.16498873276719062No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA951960.16426916219034524No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA946970.1634080933226094RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCAAGTCACCGATGTATCTCGTATGC945020.16307160348451621RNA PCR Primer, Index 2 (98% over 50bp)
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC894100.15428490473800124RNA PCR Primer, Index 1 (100% over 27bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTTGGAATTCTCGGGTGC881710.15214690007442466No Hit
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT810860.1399211026237062RNA PCR Primer, Index 1 (100% over 30bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTG793910.13699622941566555No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTATGGAATTCTCGGGTGCCAAG793270.13688579172521448No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC767820.13249416794087027RNA PCR Primer, Index 2 (100% over 50bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG763110.13168141556270677No Hit
GCCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA739540.1276142024940627No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC730280.12601630716034845No Hit
AAAACGTAATGTTACGGTGCCCAAATTAACAACTCTGGAATTCTCGGGTG715460.12345898439084035No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGC695440.12000435538641716No Hit
ATAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA694870.11990599681835916No Hit
AGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCC688780.11885511317016048No Hit
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC682190.1177179500762969RNA PCR Primer, Index 1 (100% over 31bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGACACCGATGTATCTCGTATGC674900.11645999575850247RNA PCR Primer, Index 2 (98% over 50bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGCTGGAATTCTCGGGTGCCAA663300.11445831261907642No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGATGTATCTCGTATGC651250.11237897797855197RNA PCR Primer, Index 2 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATATATCTCGTATGC641140.11063440758720738RNA PCR Primer, Index 2 (98% over 50bp)
AAATGTTACGGTGCCCAAATTAACAACTCTGGAATTCTCGGGTGCCAAGG627670.10831003932099457Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCAGTCACCGATGTATCTCGTATGCC623440.10758011521066939TruSeq Adapter, Index 2 (96% over 26bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCC613070.10579067950757905No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGCC604710.10434808717606167No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAATCACCGATGTATCTCGTATGC591330.10203924921006853RNA PCR Primer, Index 2 (98% over 50bp)
AAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA582730.100555242744632No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)