FastQCFastQC Report
Tue 25 Jul 2023
SRR10212831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143788818
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACA61457804.274171027680331No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCAC36988022.57238500979958No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACAC20720781.4410564248466107No Hit
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCAGATCGGAAGAGCA19383681.3480658836767125No Hit
GCGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACAC15557031.0819360097945863No Hit
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCAGATCGGAAGAGCAC11332890.7881621225928708No Hit
CCTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCAC7099830.4937678811713996No Hit
ATGTAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACACGTCTGA6923440.48150058511504Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA6111580.42503861461605447No Hit
ACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTG4562740.317322310835047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC4392640.3054924618686274No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCA4049570.28163316566104607No Hit
CCTGGAAACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCA3905230.2715948329167015No Hit
ACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCACACGTCT2797900.19458397662049073Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2742950.19076239989677082TruSeq Adapter, Index 6 (100% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC2474320.17208014047378845TruSeq Adapter, Index 6 (100% over 50bp)
CGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACACG2335110.16239858095224066No Hit
CCTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACA2014640.14011103422520657No Hit
AAGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACAC1975740.13740567781842394No Hit
GGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAGATCGGAAGAGCACAC1444010.10042575077013291No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)