FastQCFastQC Report
Tue 25 Jul 2023
SRR10212832.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212832.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187573750
Total Bases9.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACA2041420210.883293637835784No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACAC65062343.4686271399916038No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCAC40306132.148815066074011No Hit
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCAGATCGGAAGAGCA37043731.9748888104012423No Hit
ACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTG31214841.6641369061502476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACAC29732441.5851066580478346No Hit
ATGTAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACACGTCTGA21747411.1594058337054092Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCAGATCGGAAGAGCAC21649171.1541684270853465No Hit
CCTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCAC17941110.9564829833598785No Hit
ACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCACACGTCT9904430.5280285754269987Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCT7023310.37442925782525543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACA6724300.35848832792434976No Hit
CGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACACG6686430.35646938870710854No Hit
AGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACACGTCTGAA4801990.25600543786110797Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCACACGTC4544510.24227857042896458No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA4130790.2202221792761514No Hit
AACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGAA3433780.18306292858142464Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTGGAAACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCA3423230.18250048314329698No Hit
AAGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACAC3320960.17704822769710582No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGTAGATCGGAAGAGCACACG3015240.1607495718350782No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC2961650.15789256225884485No Hit
TCGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACAC2929390.15617270540254166No Hit
GAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACACGT2798150.14917599077696106No Hit
CGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTGA2731080.14560033053665558Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGA2680320.14289419494998634Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCAGATCGGAAGAGCA2574190.13723615377951337No Hit
TGTAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACACGTCTGAA2513460.13399849392572255Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGATGTAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACACGTCT2444580.1303263383069326Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGAAACGGGGTCTAATACCGGATAACACTCAGATCGGAAGAGCACACG2410750.12852278103945783No Hit
ACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACACGTCTGA2343890.12495831639555109Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCA2028770.10815852431376992No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTAAGATCGGAAGAGCACA2008300.10706722022671082No Hit
TTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACA1995790.10640028255552815No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1939010.10337320653876142No Hit
ACCCTGTGGGAGAGTAGGACGCCGCCGAACAGATCGGAAGAGCACACGTC1880640.100261363863547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)