FastQCFastQC Report
Thu 24 Aug 2023
SRR10212833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155225446
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACA27952941.8007962431623485No Hit
GCGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACAC27021651.740800280902398No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCAC21729931.399894834252884No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACAC13839810.8915941526752No Hit
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCAGATCGGAAGAGCA9830010.6332731039471453No Hit
GAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACACGT9268970.5971295453710599No Hit
CGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACACG8189330.5275765160307544No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA7741380.49871848975070754No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTG7012700.4517751554728984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCAGATCGGAAGAGCAC6642730.4279407900686592No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCA5416990.3489756441092783No Hit
TGGAGCTGACTGGTACTAATAGGCCGAGGGCAGATCGGAAGAGCACACGT5104100.32881851085162933No Hit
AACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGAA3735760.24066672676849643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCT3556100.22909259349140476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACACGTCTGA3340740.21521857956201332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTG3151610.20303436589900345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCTG3096680.1994956419709691Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCTG2918910.1880432670813521Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC2646000.17046174246456988No Hit
ACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCACACGTCT2439280.15714433830649133Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATGTAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACACGTCT2408720.15517558893018094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTC2322530.14962301992677154No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC2314710.1491192365457916No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2122960.13676623612342528No Hit
ATGGCTGGTTGGCTACGTTCGGAAAGGAGATCGGAAGAGCACACGTCTGA2019950.13013008189391834Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCT1984570.12785081641833387Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCTGAGACCTGATGCCGAGCCGAGATCGGAAGAGCACACGTCTGAACT1896860.12220032532552685Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1884440.12140019877926458TruSeq Adapter, Index 5 (100% over 49bp)
TAAACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTG1876930.12091638635072757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACACG1876020.12085776194194345No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATC1841550.11863712087514311No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT1816680.11703493510980152No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAGTAGATCGGAAGAGCACACGT1778660.11458559442631591No Hit
AAACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGA1723790.1110507358439157Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACAC1668310.10747657958090197No Hit
GATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCTGA1651900.1064194075499709Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)