FastQCFastQC Report
Tue 25 Jul 2023
SRR10212834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162820587
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACA48112202.9549211734508734No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACAC37983712.3328567167000815No Hit
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCAGATCGGAAGAGCA14999050.921201076372486No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA12636250.7760842920926209No Hit
TGGAGCTGACTGGTACTAATAGGCCGAGGGCAGATCGGAAGAGCACACGT10719680.658373747295236No Hit
AACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGAA10150580.6234211647941056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCAGATCGGAAGAGCAC9814800.6027984655281952No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTG9207190.5654807030022562Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGACGGACGAACCTCTGGTGTGCCAGTAGATCGGAAGAGCACACGT8566730.5261453823403793No Hit
GCGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACAC7195630.44193612936673665No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCT6862950.42150382371487216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC6108850.3751890416658429No Hit
ACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTG5981940.3673945727759844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA5754770.35344240590411335TruSeq Adapter, Index 19 (97% over 40bp)
ATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCTG5362710.3293631412838476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTC4919000.30211167338439826No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCG4023420.2471075724594949TruSeq Adapter, Index 19 (97% over 36bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3764990.23123550095050324No Hit
AGTCTGAGACCTGATGCCGAGCCGAGATCGGAAGAGCACACGTCTGAACT3643210.22375610278324323Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGTAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACACGTCTGA3587300.2203222618279837Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACAC3496040.21471731949965273No Hit
ATGTCTAACCTGGGTCCGTGATCCGGATAGATCGGAAGAGCACACGTCTG3145640.19319669938298406Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC3109750.19099243267069171No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCAC3063160.18813100090346685No Hit
ATGGCTGGTTGGCTACGTTCGGAAAGGAGATCGGAAGAGCACACGTCTGA3024170.18573634057712862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCTG2972080.18253711368820946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAGATCGGAAGAGCACAC2862300.17579472305919153No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGTACAGATCGGAAGAGCACA2833650.17403511756163856No Hit
TCCACGTCGTCGGTGCCGCAGCTAACGCATTAGATCGGAAGAGCACACGT2769840.17011607997703632No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACG2613320.16050304498656548No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACACG2557870.15709745598693856No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT2451710.15057739596529032No Hit
TGGAGCTGACTGGTACTAATAGGCCGAGGAGATCGGAAGAGCACACGTCT2147890.131917593442898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATC2046420.1256855805341127No Hit
ATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTCT2009390.12341129810568734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGA2004210.12309315651834617Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGT1994700.12250907804428933No Hit
ACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCACACGTCT1943290.11935161491587055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCTGA1925240.11824303274376477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCAGATCGGAAGAGCAC1845140.1133235074259989No Hit
AGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACAC1843760.11323875155910106No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGAGATCGGAAGAGCACACGTC1823160.11197355528511882No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGTAGATCGGAAGAGCACACG1786310.10971032796976711No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG1719800.1056254636890604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCT1717180.10546455037654422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACACGT1704030.10465691294922061No Hit
TGGCTGGTTGGCTACGTTCGGAAAGGAGATCGGAAGAGCACACGTCTGAA1701290.104488629561322Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGGGCGTAAAGAGCTCGTAGGCGGCTTGAGATCGGAAGAGCACACGTC1649650.10131704045508692No Hit
CGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTGA1640930.10078148164396435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)