FastQCFastQC Report
Tue 25 Jul 2023
SRR10212835.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212835.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178011502
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCA102837615.777020520842524No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACAC56436753.1703990678085514No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC49457122.7783103588441156No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACA48570232.7284882973460896No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCT19172931.0770613013534374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTG15808720.8880729516006219Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCTG12787800.7183693107650988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGAA12016170.6750221117734291Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTC11351990.6377110395933854No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC10405550.5845436886432204No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA10157430.5706052634733682No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACAC7371110.4140805463233494No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGT6407260.3599351686836506No Hit
GATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCTGA6352710.3568707599579717Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGAGCTGACTGGTACTAATAGGCCGAGGGCAGATCGGAAGAGCACACGT5966510.3351755326461995No Hit
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCAGATCGGAAGAGCAC5780290.3247144108699223No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCAC5692350.31977428065294344No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG5116270.2874123268731253TruSeq Adapter, Index 7 (100% over 49bp)
ATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTCT4838010.27178075268417207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTG4790530.26911350930570765Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACAC4501910.2528999502515292No Hit
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCAGATCGGAAGAGCA4490500.25225898043374745No Hit
GTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCAAGATCGGAAGAGCA4313240.2423011969192867No Hit
AAGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACAC4122410.23158110311321342No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATC3572430.20068534672551663No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCAGATCGGAAGAGCACACG3522420.1978759776994635No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC3406000.1913359508645683No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT3356350.18854680525082027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGCTGACTGGTACTAATAGGCCGAGGAGATCGGAAGAGCACACGTCT3259150.1830864839284374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC3241120.18207362802882254TruSeq Adapter, Index 7 (100% over 50bp)
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGC3042700.17092715727998295No Hit
GCGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACAC2903310.1630967643877304No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACG2901360.162987220904411No Hit
CGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACACGTC2585380.14523668251504332No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACA2573520.14457043343188014No Hit
ATGTCTAACCTGGGTCCGTGATCCGGATAGATCGGAAGAGCACACGTCTG2550140.14325703515495308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACG2544890.14296211039217005No Hit
ATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTGA2433380.1366979084306586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGATCGGAAGAGCACA2394230.13449861234247662No Hit
AAGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCA2294310.1288854919048995No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCA2261700.12705358780692722No Hit
AGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCTG2188780.12295722329223424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGTTGGGAACTAGGTGTTGGCGAGATCGGAAGAGCACACGTCTGAACT2005820.11267923574960904Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACACGTCTGAACTCCAG1990490.11181805544228261Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC1904000.1069593806359771No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCCAGATCGGAAGAGCACAC1838600.10328546073387998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)