FastQCFastQC Report
Wed 12 Jul 2023
SRR10212836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191018264
Total Bases9.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCA81142204.247876527660203No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACA48498922.5389676874039644No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACAC43366912.2703017550196143No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC34170981.788885485840244No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCT14140960.7402936087828753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGAA14067120.7364280098367976Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCTG11426710.5981998663750813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTG10594010.5546071762017479Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA10307320.5395986637173081TruSeq Adapter, Index 13 (97% over 40bp)
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATC10192330.5335788205048287No Hit
TGGAGCTGACTGGTACTAATAGGCCGAGGGCAGATCGGAAGAGCACACGT9154990.4792730186261142No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTC9125600.4777344222958701No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCAC8498380.44489881868050063No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA7797430.4082033747307011No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACAC6892650.36083722339765373No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCG6697160.3506031234793339TruSeq Adapter, Index 13 (97% over 36bp)
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCAGATCGGAAGAGCAC5789210.3030710194288019No Hit
TGGAGCTGACTGGTACTAATAGGCCGAGGAGATCGGAAGAGCACACGTCT5366860.28096056825225885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCAGATCGGAAGAGCA4847780.25378620339676No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4746980.24850922108683804No Hit
ATGTCTAACCTGGGTCCGTGATCCGGATAGATCGGAAGAGCACACGTCTG4639210.24286735220250982Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCTGAGACCTGATGCCGAGCCGAGATCGGAAGAGCACACGTCTGAACT4543390.23785107794718519Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCTGA4447810.23284736793545563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTG4418650.2313208123386568Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTCT4162160.21789330050659447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCTG3653400.1912591981256829Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCTGAGACCTGATGCCGAGCCAGATCGGAAGAGCACACGTCTGAACTC3567480.1867611989186542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACAC3536360.18513203533249575No Hit
GTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCAAGATCGGAAGAGCA3295980.17254789835175133No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGT2934760.1536376647208981No Hit
ATGGCTGGTTGGCTACGTTCGGAAAGGAGATCGGAAGAGCACACGTCTGA2882460.15089970663747632Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACAC2566640.13436620908668714No Hit
GCGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACAC2551080.1335516272936079No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC2498110.1307785940301499No Hit
AACGTTGGGAACTAGGTGTTGGCGAGATCGGAAGAGCACACGTCTGAACT2478680.12976141380910047Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGC2385790.12489852802766545No Hit
ATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTGA2373960.12427921552045934Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGATCGGAAGAGCACA2344320.12272753143647039No Hit
AAGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCT2256940.11815309974757178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACACGTCTGAACTCCAG2086580.10923458083568385Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA2079810.10888016446427343No Hit
CGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACACGTC2031340.10634271076822267No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCAGATCGGAAGAGCACACG2017690.10562811941375408No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACA2017420.10561398463970963No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCA2007180.10507791024632074No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC1976320.10346235792405693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)