FastQCFastQC Report
Wed 12 Jul 2023
SRR10212837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207591209
Total Bases10.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCA68228343.2866680785119375No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC61658422.97018454187046No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACA46999642.2640477034843993No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACAC41477681.9980460733286638No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTG28389391.367562245855989Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCT22484251.083102223273819Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGAA20526480.9887933163874969Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA16878660.8130720024854232TruSeq Adapter, Index 16 (97% over 40bp)
ATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCTG14327020.6901554294623333Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC13266390.6390631888463061No Hit
GCGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACAC12558850.6049798573117804No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA12176020.5865383249441936No Hit
ACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTG11285980.5436636770105231Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCAGATCGGAAGAGCA9518980.45854446562811824No Hit
AGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCTG9424670.4540014023426204Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTC9390990.452378982965507No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCG9252420.4457038448097289TruSeq Adapter, Index 16 (97% over 36bp)
CTGGAAACGGGGTCTAATACCGGATAACACTCTGTAGATCGGAAGAGCAC8471310.408076528905422No Hit
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCAGATCGGAAGAGCAC8332990.40141343364882087No Hit
TGGAGCTGACTGGTACTAATAGGCCGAGGGCAGATCGGAAGAGCACACGT8296860.3996729938597737No Hit
GATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCTGA8148810.39254118896720713Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACAC7243520.3489319241837452No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGT6343350.3055692979754263No Hit
AGTCTGAGACCTGATGCCGAGCCGAGATCGGAAGAGCACACGTCTGAACT6188030.29808728557479525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCT5647930.2720698061929973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACACGTCTGA5052290.24337687633005692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCTGAGACCTGATGCCGAGCCAGATCGGAAGAGCACACGTCTGAACTC4874510.23481292986737215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACACGT4716280.22719073812032184No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCAGATCGGAAGAGCACACG4568690.22008109216224084No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC4542710.21882959407977629No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATC4417680.2128066993434197No Hit
ATGGCTGGTTGGCTACGTTCGGAAAGGAGATCGGAAGAGCACACGTCTGA4178420.20128116311514907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT3896620.1877064071629353Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCAAGATCGGAAGAGCA3819900.18401068226352493No Hit
AACGTTGGGAACTAGGTGTTGGCGAGATCGGAAGAGCACACGTCTGAACT3799740.18303954287389887Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAGCTGACTGGTACTAATAGGCCGAGGAGATCGGAAGAGCACACGTCT3763020.1812706818427942Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACAC3745240.18041419085333232No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC3516260.16938385863921626No Hit
ATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTCT3424360.1649568889017839Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTGA3319120.15988731006427157Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACAC3270970.15756784768279855No Hit
AAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGATCGGAAGAGCACA3237990.1559791484233805No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACA3050050.1469257785381461No Hit
GTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCAGATCGGAAGAGCAC3024190.14568006104728642No Hit
TGGCTGGTTGGCTACGTTCGGAAAGGAGATCGGAAGAGCACACGTCTGAA2819510.1358202986331661Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTATAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG2716060.13083694695376044Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTGA2683520.12926944319689376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGTTGGGAACTAGGTGTTGGCGAAGATCGGAAGAGCACACGTCTGAAC2680740.12913552615804652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGTACCGCCGAAGTCGTGTCATTCGAGATCGGAAGAGCACACGTCTGA2610790.1257659229683469Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA2604560.12546581392085826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACACG2524190.12159426269346503No Hit
CGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACACG2503350.12059036661807773No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT2449300.11798669181602964No Hit
ATGTCTAACCTGGGTCCGTGATCCGGATAGATCGGAAGAGCACACGTCTG2401500.11568408949340432Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACG2362260.11379383603859641No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACG2277850.10972767156050428No Hit
GCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCTGA2188730.10543461886191915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACACGTCTGA2187290.10536525176266015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAAGATCGGAAGAGCA2110040.10164399591699473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)