FastQCFastQC Report
Tue 25 Jul 2023
SRR10212838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146481080
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC33626242.2956029543201075No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTAGATCGGAAGAGCACAC19977801.363848491559456No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCA19245181.3138338412032462No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACA18633671.2720871528254707No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTG13017490.888680640530504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGAA10657360.7275588082774922Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCT9928600.6778076731820929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC9189430.6273458661009326No Hit
ATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCTG6365750.43457830867986497Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAGCTGACTGGTACTAATAGGCCGAGGGCAGATCGGAAGAGCACACGT5518270.3767223726094865No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGT4512770.3080786952144263No Hit
AGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCTG3902540.2664193901355724Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA3886890.26535099276985125TruSeq Adapter, Index 18 (97% over 40bp)
GATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACGTC3791110.25881226435523275No Hit
ACGGGGTCTAATACCGGATAACACTCTGAGATCGGAAGAGCACACGTCTG3613410.24668100480963137Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCTGA3530590.24102703229659422Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCG3343200.22823425387087534TruSeq Adapter, Index 18 (97% over 36bp)
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCAGATCGGAAGAGCA2917560.19917657625134932No Hit
CTGGAAACGGGGTCTAATACCGGATAACACTCAGATCGGAAGAGCACACG2680280.1829778972137562No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC2679570.18292942679013563No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA2507790.1712023150020467No Hit
GCGAGTCAGAAACCGTTGATGTAGGCGAAGGACAGATCGGAAGAGCACAC2496910.170459556961213No Hit
TGGAGCTGACTGGTACTAATAGGCCGAGGAGATCGGAAGAGCACACGTCT2421970.165343537882162Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACAC2393900.16342724944409204No Hit
AGTCTGAGACCTGATGCCGAGCCGAGATCGGAAGAGCACACGTCTGAACT2343160.15996332086027765Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCCTGCTTCGAGATGAGTATTCCCACCAGATCGGAAGAGCACACGTCT2312650.1578804580086384Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACACGTCTGA2167690.14798429940576627Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCAGATCGGAAGAGCAC2151220.14685992211417337No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATCGGAAGAGCACAC2148260.14665784823541717No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT2140470.14612603893963644Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGTTGGGAACTAGGTGTTGGCGAGATCGGAAGAGCACACGTCTGAACT2042670.13944940875640732Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGATCGGAAGAGCACA2013640.13746758284414615No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACA1958260.13368688980174095No Hit
AGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACG1908030.13025777800109067No Hit
ATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTGA1839060.12554932008966618Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT1734010.1183777454398889No Hit
ACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGAAC1694930.11570982409468854Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACG1644660.11227798156594694No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGCTGAAACAGATC1643030.11216670439622646No Hit
AAGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACAC1601930.10936088128241546No Hit
AGTCTGAGACCTGATGCCGAGCCAGATCGGAAGAGCACACGTCTGAACTC1580580.10790335516368393Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCA1476920.10082667331507932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)