FastQCFastQC Report
Tue 25 Jul 2023
SRR10212842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166241490
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT1705623010.259911650214395No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC154690609.305174057330694No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC49301702.965667595977394No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCA31885341.9180133671804795No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGT27573721.6586545272182052No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCGGAGATCGGAAGAGCACACGT21646491.302111163705282No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGGAGATCGGAAGAGCACACGT21127071.270866256071213No Hit
TGGCCTATGGTGTAATTGGCAGCACGACGGAGATCGGAAGAGCACACGTC14905910.8966419875086538No Hit
GGGCCTGTGGCGCAGTCTGGTAGCGCACCTCGAGATCGGAAGAGCACACG14468060.8703037972048975No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACAC14441200.8686880754016342No Hit
GCACTCGTAGCTTAACGGATAGAGCATCTGAGATCGGAAGAGCACACGTC13169130.7921686698067973No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGAGATCGGAAGAGCACACG11959510.7194058474812756No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACAGATCGGAAGAGCACACGTC10649130.6405819630225884No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAGCAGATCGGAAGAGCACACGT10621690.6389313522153826No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTCCAGATCGGAAGAGCACACGT9267200.557454098853421No Hit
GCCCTTTTAGCTCAGTCGGTAGAGCGTCTCCAGATCGGAAGAGCACACGT9118220.5484924371166308No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGGCAGATCGGAAGAGCAC8421080.5065570574469707No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCCAGATCGGAAGAGCACACG8004890.4815217909800977No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA7750420.4662145412676463No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGGCAGATCGGAAGAGCAC6935750.41720932602324484No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGAGATCGGAAGAGCACACGTC6115100.36784439311750633No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGAGATCGGAAGAGCACACGT5893230.3544981460404379No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTC5825150.3504028988190614No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC5733410.3448844208506553No Hit
GCGGCCGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT5463790.3286658462938464No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC5456240.3282116877080445No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCAGATCGGAAGAGCACACGTCT5066980.30479635378629005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTCCAGATCGGAAGAGCA4768620.2868489689306803No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGGAGATCGGAAGAGCACA4360540.2623015469844502No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTTGAGATCGGAAGAG4346770.26147323390809357No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACA4285510.2577882332503155No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAGAGATCGGAA4262610.2564107191291416No Hit
GCGGAAGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT3923400.23600606563379578No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACTAGATCGGAAGAGCACACGT3600750.21659755335446043No Hit
TGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTCCAGATCGGAAGAGCAC3483720.20955779450725567No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC3416810.20553292682831464No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA3362030.2022377205594103TruSeq Adapter, Index 19 (97% over 40bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACG3343070.20109721105122433No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGGAGATCGGAAGAGCACA3167800.19055411498056232No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAGCAGATCGGA3166560.19047952469627166No Hit
GGTAGCGTGTCCGAGCGGCCGAAGGAGCTCGCAGATCGGAAGAGCACACG3083370.18547535876874058No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCG3024520.1819353279376887TruSeq Adapter, Index 19 (97% over 36bp)
GGGGCTATAGCTCAGTTGGTTAGAGCGCATAGATCGGAAGAGCACACGTC3020540.1816959171864978No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTTGAGATCGGAAGA2987140.1796867917870563No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCGGAGATCGGAAGAGCACACGT2760920.1660788771804199No Hit
TCCAGGATGTCGCGGGTTCGAGTCCCGTTACTCACCCCAGATCGGAAGAG2677830.16108072659839612No Hit
TGGTGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAG2633270.1584002886403388No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAGATCGGAAGAGCACA2587790.1556645095036143No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACCGGAGATCGGAAGAGCACACGTC2521480.1516757338977171No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACCTAGATCGGAAGAGCACACG2418140.1454594758504631No Hit
TAGGACGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT2277690.13701092308544635No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAGCAGATCGGAA2268950.1364851818881075No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGTACAGATCGGAAGAGCACA2245680.13508541098855648No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCAGATCGGAAGAGCACACGTCTG2228750.1340670129941689Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGTATGGATAGGCGAAGGACATGCGAAAGGTCCGGCAGATCGGAAGAG2170810.13058172180723357No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTAGATCGGAAGAGCACACGTCT2116730.12732862295688038Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGT2114560.12719808995937174No Hit
TCGGACGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT2078680.12503978399134896No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTCCAGATCGGAAGAGCACA2070440.12454411952154663No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT2061450.12400333996043947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCGGAGATCGGAAGAGCACA2001880.12041999864173498No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGCTAGATCGGAAGAGCA1978110.11899015101464742No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAGATCGGAAGAGCACA1897910.1141658439177849No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCCCAGATCGGAAGAG1843290.11088026220169224No Hit
GGCTCTGTGGAGCAGTTGGTTAGCTCGCCACAGATCGGAAGAGCACACGT1802050.10839953371447766No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGAGATCGGAAGAGCACACGTC1785710.10741662625858323No Hit
GAGGCCGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT1764870.10616302825485985No Hit
AAGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAAGATCGGAAGAGC1747460.10511575660203719No Hit
GGACGATTAGCTCAGCGGGAGAGCACTTCCCAGATCGGAAGAGCACACGT1730220.10407871103657697No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAGATCGGAAGA1723070.10364861383280431No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGAGATCGGAAGAGCACACGT1710140.10287082965870914No Hit
ACGGGGTCTAATACCGGATAACACCGGCCAGATCGGAAGAGCACACGTCT1694590.10193544343232246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTCCGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT1666580.10025054515572496No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)