FastQCFastQC Report
Thu 24 Aug 2023
SRR10212846.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10212846.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168577692
Total Bases8.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC1702701010.100393354537088No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT146023788.6621057785036No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCAC53715253.1863794884556844No Hit
TGGCCTATGGTGTAATTGGCAGCACGACGGAGATCGGAAGAGCACACGTC23902061.4178661314214696No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGGAGATCGGAAGAGCACACGT22621791.3419207329045648No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCCAGATCGGAAGAGCACACG22547311.337502591979964No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGAGATCGGAAGAGCACACG21893911.2987430151790191No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCA18480191.0962417257438783No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCGGAGATCGGAAGAGCACACGT18323161.0869267328680714No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTCCAGATCGGAAGAGCACACGT18250341.0826070628609625No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGT17575811.0425940580560327No Hit
GGGCCTGTGGCGCAGTCTGGTAGCGCACCTCGAGATCGGAAGAGCACACG16628220.9863831805218927No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACAC15829580.9390079916386564No Hit
GCCCTTTTAGCTCAGTCGGTAGAGCGTCTCCAGATCGGAAGAGCACACGT14684150.87106127897397No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCCCAGATCGGAAGAG12955350.7685091571902646No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC12754170.7565751938281371No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA11710960.6946921541671125TruSeq Adapter, Index 18 (97% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCG11059980.6560761313543194TruSeq Adapter, Index 18 (97% over 36bp)
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC10037160.5954026230232171No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTC9736840.5775876917332573No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCAGATCGGAAGAGCACACGTCT8502460.5043644802065507Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGAGATCGGAAGAGCACACGT8256230.4897581585112697No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAGCAGATCGGAAGAGCACACGT8092400.4800397907927224No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACAGATCGGAAGAGCACACGTC7566150.4488227303527207No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCGGAGATCGGAAGAGCACA7426060.4405126153939751No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTTGAGATCGGAAGAG6990380.4146681519402935No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGGCAGATCGGAAGAGCAC6935120.4113901381447315No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGAGATCGGAAGAGCACACGT6763520.4012108553485238No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCGAGATCGGAAGAGCACAC6674120.39590766256308696No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC6580730.3903677836566893No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTTGAGATCGGAAGA6303280.3739094968745924No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAGTAGATCGGAAGAGCACACGT6191650.36728762427237405No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGGCAGATCGGAAGAGCAC6081210.3607363422676353No Hit
AGACCCCGGGACCTTTACTACAGTTTGATATTGGTAGATCGGAAGAGCAC5869720.348190791460118No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCA5850400.3470447323480974No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGAGATCGGAAGAGCACACGTC5721600.3394043382679601No Hit
ATGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTCCAGATCGGAAGAGCA5656270.3355289737861638No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGTACAGATCGGAAGAGCACA5619350.333338885669404No Hit
ACGGGGTCTAATACCGGATAACACCGGCAGATCGGAAGAGCACACGTCTG5523380.32764596160208437Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGACGTGGCTCAGTTGGTAGAGCATCACTAGATCGGAAGAGCACACGT5251930.31154359379887586No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGC4930940.2925025216266456No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACACG4895540.29040259965120413No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAGATCGGAAGAGCACA4806490.28512016880620245No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC4702640.27895980447994273No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTAGATCGGAAGAGC4480150.2657617355444634No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCGCATAGATCGGAAGAGCACACGTC4456600.26436475355232647No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAGATCGGAAGA4293690.25470096007720877No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGT4187030.24837390702916964No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAG4175910.2477142705216299No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACCTAGATCGGAAGAGCACACG4026690.2388625655166758No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT3944040.2339597815824884No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA3925900.23288371986964918No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGATCGGAAGAGCA3666350.21748725804123598No Hit
ACCGAGATCTACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC3545580.21032320219451103TruSeq Adapter, Index 18 (96% over 29bp)
GCCCTCATCGTCTAGTGGCCCAGGACGCCGAGATCGGAAGAGCACACGTC3529710.20938179649535124No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT3483470.20663884756471812Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGTAGCTTAACGGATAGAGCATCTGAGATCGGAAGAGCACACGTC3452870.20482366077238737No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCAGATCGGAAGAGCACA3444540.20432952659003067No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTG3392900.201266250578398Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAG3335900.19788502027895835No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTCGAGATCGGAAGA3327850.1974074956489498No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGGAGATCGGAAGAGCACA3317820.19681251775590805No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGATCGGAAGAGCACACGTCTGAAC3043160.18051973329899426Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGACGTGGCTCAGTTGGTAGAGCATCAGATCGGAAGAGCACACGTCTG3019850.17913698806601291Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTAGATCGGAAGAGCACACG2880020.1708422962630192No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG2750950.16318588582883198Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTCCAGATCGGAAGAGCAC2581220.15311753111437781No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTAGATCGGAAGAGCACACGTCT2533420.1502820432492337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGTATGGATAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCACA2476760.1469209816919311No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGGAGATCGGAAGAGCACA2454730.14561416584111259No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTCCAGATCGGAAGAGCACA2368940.1405251176413069No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGTAGATCGGAAGAGCACACGTCT2366360.14037207248038489Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCTGTAGCTCAATTGGCAGAGCAGCAGATCGGAAGAGCACACGTCTG2303030.13661534765821803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCTGTGGAGCAGTTGGTTAGCTCGCCACAGATCGGAAGAGCACACGT2265610.13439559962655082No Hit
GTTTCTTGCCGGAGGTAGAGCACTGGATAGGAGATCGGAAGAGCACACGT2225950.13204297517609861No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACCGGAGATCGGAAGAGCACACGTC2222650.13184721973771002No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGCTAGATCGGAAGAGCA2188540.12982382034272957No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTCCAGATCGGAAGAGCACA2165060.1284309907386797No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACA2157300.1279706688593174No Hit
AACCGTATGGATAGGCGAAGGACATGCGAAAGGTCAGATCGGAAGAGCAC2136660.12674630757194139No Hit
GGACGATTAGCTCAGCGGGAGAGCACTTCCCAGATCGGAAGAGCACACGT2026400.12020570313656923No Hit
AACGTTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGAA1943990.11531715596153731Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAGCAGATCGGAA1837540.10900256007775927No Hit
ACGGGGTCTAATACCGGATAACACCGGCCAGATCGGAAGAGCACACGTCT1808190.10726152307269696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGGTGGAGCAGCTCGGTAGATCGGAAGAGCACACGTCTGAACTCCA1795550.10651172042383877Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCTTGCCGGAGGTAGAGCACTGGATAGGCAGATCGGAAGAGCACACG1766960.10481576648943562No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCGGAGATCGGAAGAGCACACGT1743450.10342115729049131No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAGATCGGAAGAGCACACGTCTG1732890.10279473988764776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA1703310.10104005932172805Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTAGATCGGAAGAGCA1699920.1008389650986561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)