FastQCFastQC Report
Tue 18 Jul 2023
SRR10251308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10251308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25365678
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG18482567.286444304780656No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG11197474.414417781381597No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA9509163.7488294221822103No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5641762.224170786998085No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT3122291.2309113125223778No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC1833680.7228980829923016No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC1729860.6819687610952091No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1635550.6447885997764381No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1599780.6306868675065574No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1579310.6226169077759325No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1568560.6183788976584816No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1483140.5847034721484676No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1336670.526960091506326No Hit
TCGGCATAGAGGGTGATACCCCCGTATGTGCTGTAGGCACCATCAATAGA886050.34931059205277304No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG858910.33861109488183205No Hit
TGAGCTGACCACAATACTAATAAATCGCTGTAGGCACCATCAATAGATCG746080.2941297291560667No Hit
TTGAAGTTACAAAACTTAATGATAGCAGAAGCTTCTGTAGGCACCATCAA734180.28943835051442346No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG718880.2834065779751679No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA648830.25579052134936037No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA646170.25474186024122836No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA640140.25236463224046285No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA626540.24700305665001343No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT613750.24196081019399518No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA603390.2378765511412705No Hit
TTCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA574040.2263057979368815No Hit
TTGAGCTGACCACAATACTAATAAATCGCTGTAGGCACCATCAATAGATC573780.22620329722706406No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA564190.22242259796879862No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG556240.21928844164938152No Hit
ATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG543400.21422648351839838No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT534040.21053645796497142No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGGCGGCTGTAGGCA519630.2048555532400908No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA469830.18522272497506276No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA443660.1749056342984406No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT410570.16186044780667797No Hit
TCCTCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA407440.16062649695387604No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA400020.1577012843890867No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA399580.1575278216493957No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT374240.14753794477719065No Hit
TCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAGATC356020.1403550104199856No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAAT347180.1368699862861935No Hit
ATGTACACGTAGGGATAACAGGGTAATAACGCGACTGTAGGCACCATCAA346360.13654671481676933No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT339120.13369246428185363No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG338660.13351111687217665No Hit
TTGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC336750.13275813088851793No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA314250.12388787715431851No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA308830.1217511315881247No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA306130.12068670114002078No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT302940.11942909627726095No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC301770.11896784308308259No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGTAGGCACCATC299510.11807687537466965No Hit
GCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA293220.11559714666408681No Hit
TGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT291900.11507675844501376No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATCAATAGA291050.11474165997061068No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATCG287820.11346828576787894No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT268480.10584380989146043No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGTAGGCACCATCAATAG266140.10492130350310369No Hit
TCGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAGAT259690.10237849743263319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)