FastQCFastQC Report
Tue 18 Jul 2023
SRR10251309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10251309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37498632
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG16486464.3965497194670995No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA16167184.3114052800646165No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA15323824.086501075559236No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG11806733.1485761933928686No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT10331182.75508183871881No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG4282991.1421723331133786No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA3961581.0564598729895No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2778680.7410083653185001No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC2713990.7237570693245556No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2424090.6464475824078063No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2156840.575178315838295No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC2011910.5365289059078208No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2007560.5353688635894771No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1743430.4649316273724332No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT1636670.43646125543993175No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1541580.41110299703733194No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1483840.3957051019887872No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1424420.37985919059660633No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1389860.3706428543846613No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC1100090.2933680353992647No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGTAGGCACCATC1045130.2787115007288799No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1020740.2722072634543042No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT754020.20107933537415446No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT716600.1911003046724478No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA651600.17376633899604657No Hit
TCCTCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA633250.16887282714740098No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA624570.1665580760386139No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCA598550.15961915624015297No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC594970.1586644547459758No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA592330.15796042906311888No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA587810.15675505175762144No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC580010.1546749758764533No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA554420.14785072692785167No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA552590.14736270912496222No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCA551210.14699469569983248No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA530020.14134382288932568No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG469000.12507122926511025No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA468630.1249725589989523No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG463610.12363384349594407No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA463570.12362317644014321No Hit
TTAGCACTCTGGACTCTGAATCCAGCGATCCGAGTCTGTAGGCACCATCA463520.12360984262039212No Hit
TTGAAGTTACAAAACTTAATGATAGCAGAAGCTTCTGTAGGCACCATCAA458420.12224979300578219No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG435770.11620957265854391No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC427160.11391348889740831No Hit
CAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT423170.1128494500812723No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA417390.11130806051804769No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT416220.1109960491358725No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT403630.10763859332255107No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA390290.10408113021296352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)